Báo cáo khoa học: Regulation of DNp63a by tumor necrosis factor-a in epithelial homeostasis docx

... DNp63a by TNF-a was attenuated by inhibition of NF-jB, sug- gesting that activation of NF-jB may be involved in the regulation of the degradation of DNp63a. Interest- ingly, knockdown of DNp63a ... Ij-Ba, inhibitor of kappa B; JNK, c-jun N-terminal kinase; NF-jB, nuclear factor-jB; si, small interfering; TA, transactivating; TNF-a, tumor necrosis factor-a; 7AAD,...

Ngày tải lên: 16/03/2014, 06:20

... activation of protein kinase C (PKC) [9]. Links between retinoids and PKC were con- vincingly demonstrated [10], and even pointed to direct binding between PKCs and RA receptors and control of transcriptional ... 2009) doi:10.1111/j.1742-4658.2009.06992.x One of the emerging approaches for the treatment of Alzheimer’s disease aims at reducing toxic levels of Ab-species through the m...

Ngày tải lên: 16/03/2014, 01:20

... aliquots were incubated in the presence of increasing concentrations of calmodu- lin, followed by the immediate determination of PMCA activity and the amount of acetylated tubulin remaining in the membrane ... acetylated tubulin. Tubulin-free PMVs were then incubated with purified brain tubulin containing two different proportions (differing by a factor of four) of the acet...

Ngày tải lên: 23/03/2014, 07:20

... inhibitors, binding revers- ibly into the active site of the enzyme. Cystatins, including human cystatin C, are general inhibitors of cysteine proteases. For cathepsin B, their K i value is in nanomolar ... nm, depicting a strong interaction between 2A2 mAb and cathepsin B. Determination of the 2A2 mAb binding site on cathepsin B The binding site of 2A2 mAb on cathepsin B was determ...

Ngày tải lên: 18/02/2014, 11:20

... dCTP(dUTP)-binding conformer and the dTTP-binding conformer of dCTP deaminase. We were not able to identify structural changes in the main chain of the subunit, or in the interaction of subunits within ... of dCTP binding by dTTP. However, the presence of dTTP in the assay incubation also increased the apparent cooperativity of dCTP bind- ing, which indicates that the mech...

Ngày tải lên: 18/02/2014, 16:20

... modification of the actin–HMM interface, which results in the inhibition of HMM cross- linking to the N-terminal part of a ctin. This change of the actin–HMM ionic interface would be in contrast ... and T m–Tn binding to actin induces a shortening of filament length and an increase in myosin sliding velocity, suggesting a strong synergy between these two actin binding components...

Ngày tải lên: 19/02/2014, 16:20

... was inhibited by MG-132 [20], suggesting indirectly an involvement of NF-jBin ArgII regulation. Our study is thus the first direct demonstration of the involvement of IRF-3 in ArgII regulation in ... iodide (PI) staining of apoptotic cells was quantified in VSV- infected Jurkat T cells in the absence or presence of spermine. As shown in Fig. 7, the presence of spermin...

Ngày tải lên: 07/03/2014, 21:20

... understanding of the regulation of calpain B. According to homolo- gous modeling, the amino acid residues phosphory- lated by PKA and the ERK isoforms are situated in sensitive regions of the molecule ... Myosin phosphatase interacts with and dephosphorylates the retinoblastoma protein in THP-1 leukemic cells: its inhibition is involved in the attenuation of daunorubicin-ind...

Ngày tải lên: 16/03/2014, 02:20

... the site of ATP binding and may exploit common side chain interactions; or (b) the conforma- tions of residues involved in RNA binding may be directly or indirectly coupled to the binding of ATP and ... (2001) Investigation of invariant serine ⁄ threonine residues in mevalonate kinase. Tests of the functional significance of a proposed substrate binding motif and a site impli-...

Ngày tải lên: 16/03/2014, 06:20

... certain condi- tions, by the Jun N-terminal kinase, the P38c MAP kinase, the cyclin-dependent kinases 1 and 2 and the PDZ-binding kinase, resulting in changes in distribu- tion and stability of ... short). Since DLG1 is known to be altered in cancers of epithelial origin and is the target of oncogenic HPV, we were first interested in investigating the initiation of DLG1 tra...

Ngày tải lên: 22/03/2014, 16:20