Báo cáo khoa học: Effects on protease inhibition by modifying of helicase residues in hepatitis C virus nonstructural protein 3 pot

... (5¢-TCCCGTGTGT GCAGACCATCTTGAAT -3 ), H528A forward primer (5¢-TCCCGTGTGTCAAGACGCTCTTGAAT -3 ) or H 52 8S forward primer (5¢-TCCCGTGTGTCAAGACTCTCTTG AAT -3 ) and reverse primer (5¢-AGTCCCGGGGTGTT CATGTATGCTC -3 ). ... this is not considered to be of great importance and could be a result of differences in the active enzyme concen- tration. The effect of changing residues in the h...

Ngày tải lên: 16/03/2014, 06:20

... discourse; information on the position and on the whole sentence can be extracted from each occurrence of CFRAME. In accumulating discourse informa- tion, a score of 1.0 is awarded for each ... discourse information, and give the results of an experiment on completing incomplete parses in technical documents. 2 Discourse information for completing incomplete parses In...

Ngày tải lên: 08/03/2014, 07:20

... forward, CCTATGAGCACCTGACCACA reverse, AGGCCACTGACTAGGCTGAA Egr1 pre-mRNA forward, GAGCAGGTCCAGGAACATTG reverse, GGGATAACTCGTCTCCACCA Ndrg1 forward, ACCTGCTACAACCCCCTCTT reverse, TGCCAATGACACTCTTGAGC Idi1 ... forward, CCTTGCCCTACAGCTGAGTC reverse, CTTGTCTTCTGTGCCTGTGC Ifrd1 forward, GTTTGAATTGGCCAGAGGAA reverse, TCTGTTGGAAAATCCCGTTC Cxcl10 forward, CCCACGTGTTGAGATCATTG reverse, GAGGAACAGCAGAG...

Ngày tải lên: 07/03/2014, 03:20

... thermal convection from surface after concrete placing was adopted. Table 4 demonstrates the applied section, method of concrete curing, and curing periods. According to the table applied curing ... delayed concrete and the rest of section was placed by normal concrete. The method of curing concrete is demonstrated in 3. 3 and shown in Figure 6. Figure 6. Shape and curin...

Ngày tải lên: 07/03/2014, 03:20

... effected by incu- bation with cytosolic fraction). Table 2. Reduction in glycoconjugate bound Neu5,9Ac 2 and oligo-O-acetylated Neu5Ac in cancer mucosa at various stages of colorectal carcinoma. Sialic ... oligo-O-acetylation with mono-O- acetylation remaining constant [7 ,30 ]. In this study, utilizing matched colonic samples (resection margin and cancer tissue obtained from the sa...

Ngày tải lên: 07/03/2014, 14:20

... cloning into plasmid pKP1500. The forward p rimer 5¢-ATGACTATTTCTTCTGCACAT CCAGAGACAGAAC -3 con tains the initiation codon, while the reverse primer 5¢-CTTTCTGCAGACTCA TTCGCTGATATATATTC -3 linked ... c onstructed by a recombination o f the N-terminal fragment containing consensus region I of isomaltase and maltase, respectively. The recombination had no effect on either the substrate...

Ngày tải lên: 07/03/2014, 16:20

... first is a selection of data from CoNLL- 2004 and contains 8 936 sentences. The second dataset is part of the Lancaster Treebank corpus and contains 14 73 sentences. Each sentence con- tains hand-labeled ... Proceedings of the COLING/ACL 2006 Main Conference Poster Sessions, pages 120–127, Sydney, July 2006. c 2006 Association for Computational Linguistics Techniques to incorporate t...

Ngày tải lên: 08/03/2014, 02:21

... 5¢-ACTGGCGGCCGCTCGAG -3 with one of the sense primers AR2P()870), AR2P( )34 3) or AR2P()72) (5¢-GGTACCTTCCCCCTCCTACTGAATGT -3 , 5¢-GGTACCCCTCCTCCTCAGCTCCAAAT -3 and 5¢-GGTACCTCGTGGGGGCGGGGAGA -3 , ... [c- 32 P]dATP (sense 5¢- GTGCGATGCGCGTCACGGCGA -3 ; antisense 5¢-TC GCCGTGACGCGCATCGCAC -3 ). Labeled probes were then incubated with 5 mg of nuclear extract protein in the presence or...

Ngày tải lên: 22/03/2014, 21:21

... N-myristoylated proteins include protein kinases, phosphatases, guanine nucleotide-binding proteins, and Ca 2+ -binding proteins, many of which participate in signal transduction pathways [1,4–7]. Protein ... position 3 for N-myristoylation (Fig. 5B). In the case of Myr– AGG1-3X6S7K, [ 14 C] myristic acid incorporation was detected in all constructs, with the exception of t...

Ngày tải lên: 29/03/2014, 21:20

... concentration; FRET, fluorescence resonance energy transfer; [I], inhibitor concentration; K ðappÞ i , apparent inhibition constant; K i , competitive inhibition constant; K is , inhibition constant; MAPKKide, ... 2. Inhibition of the lethal factor (LF) protease by neomycin B: ionic strength (I.S.) effects on the apparent inhibition constant. The apparent inhibition const...

Ngày tải lên: 19/02/2014, 06:20