Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf

... conclusion that lactate produc- tion in strain FI9078 was mediated by the enzyme encoded by the ldhB gene. As expected, the enzyme pro- duced by strain MG1363 was encoded by the ldh gene. LDHB (ldhB gene ... 50% of the maximum rate; LDH, L -lactate dehydrogenase encoded by the ldh gene; LDHB, L -lactate dehydrogenase encoded by the ldhB ge...

Ngày tải lên: 16/03/2014, 05:20

... plasmid combinations were spotted either onto medium lacking histidine and leucine to select for the plasmids, or onto medium lacking histi- dine, leucine and uracil to monitor protein interactions. The ... acyltransferase was determined in an are1 deletion background. The enzyme activity in the ste20D strain and the cla4D strain was indistinguishable from that in t...

Ngày tải lên: 18/02/2014, 13:20

... RI binding. The affinity of porcine ribonuclease inhibitor (pRI) for the 1C zymogen was determined by using a competitive binding assay with fluorescein-labeled G88R RNase A [36]. The resulting ... v), and dithiothreitol (0.005 m) [48]}, and incubating the resulting mixture at 37 °C for 15 min. Activation was stopped by dilution (1 : > 1 0) into 0.10 m Mes ⁄ NaOH buffer, p...

Ngày tải lên: 07/03/2014, 11:20

... eval- uated by the clinical pharmacist, comprising 1,286 in the C-U and 1,224 in the PB-U. In the C-U, 44 MPEs occurred versus 331 in the PB-U (3.4% versus 27.0%, P < 0.00 1). Overall, the ICIS ... inci- dence of minor MPEs decreased from 18.3% in the PB-U to 0.7% in the C-U, since completeness and legibility of the order was mandatory in the CPO...

Ngày tải lên: 25/10/2012, 10:39

... nursing handover (red, up-pointing arrows), the beginning and end of the daily medical shift (green, down-pointing arrows), and periods of routine nursing observations (pink, shorter, up-pointing ... hours' (18:00–08:0 0) when wards are not staffed by parent unit doc- tors. In addition, there was a trend toward increased frequency of activation of the MET service duri...

Ngày tải lên: 25/10/2012, 10:45

... 1. Solution : The number contains the end digit is digit 1 The number contains the end two digits are digit 1 The number contains the end three digits are digit 1 The number contains the end four ... Z, T) = 0 =”, Str4 : Lbl 1 : Input « Xo = », A : Input « Yo = », B : Input « Zo = », C : Input « To = », D : 1 → N : String  Equ(Str1, Y 1) : String  Equ(Str2, Y 2) : St...

Ngày tải lên: 21/10/2013, 16:11

... °C) for 4 weeks, and then transferred to a growth chamber with a tempera- ture regime of 22 °C in the day (16 °h) and 16 °C at night (8 h). The light intensity was 50 lmolÆm )2 Æs )1 , and the ... are reversibly interconnected. The rate of sucrose export to sink organs (r Suc fi Sinks ) was calculated as the difference between the spline-interpo- lated rates...

Ngày tải lên: 14/02/2014, 22:20

... killing. In addition to actin, other host cell proteins have been shown to bind to the virulence factor YpkA, including OTUB1 [13]. In order to investigate the role of OTUB1 in Yersinia invasion of HEK293T ... susceptibility to Yersinia invasion Yersinia virulence factors are injected into target host cell molecules to manipulate signalling pathways dur- ing invasion in...

Ngày tải lên: 16/02/2014, 15:20

... mechanism include, but are not limited to, acetylation (lysine), phosphoryla- tion (serine, threonine), methylation (lysine, arginine), sumoylation (lysine), and ubiquitination (lysine). Other epigenetic ... possible to distin- guish the respective cases? In the first case, the cell lines were specifically treated to put them into mitosis; this was not so in the second and...

Ngày tải lên: 18/02/2014, 08:20

... explain the suppression of I Ks by KCNE4. Next, we examined the ability of KCNE proteins to traffic to the plasma membrane in the presence of KCNQ1. Total protein, non-biotinylated and biotiny- lated ... Health, Bethesda, MD, USA). The percentage of protein at the membrane was calculated by dividing the biotinylated fraction value by the total protein fraction val...

Ngày tải lên: 18/02/2014, 17:20