Báo cáo khoa học: Dual effect of echinomycin on hypoxia-inducible factor-1 activity under normoxic and hypoxic conditions docx
Báo cáo khoa học: Dual effect of echinomycin on hypoxia-inducible factor-1 activity under normoxic and hypoxic conditions docx
... has a dual effect on HIF-1 activity under normoxic and hypoxic conditions, demonstrating thatthis molecule cannot be used in cancer treatment. Inthe context of cancer treatment, the use of this ... basal and PMA-stimulatedc-myc transcriptional activity (Fig. 2C). AP-1 activity was also low in unstimulated HepG2 cells. PMAenhanced this activity and, under normoxic and hypoxic conditions, echinomycin ... BNIP3 and aldolase overexpression induced by 16 h of incubation under hypoxic conditions. Again, a slight increase inHIF-1 activity was observed in the presence of 2 nm echinomycin under normoxic...
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Báo cáo khoa học: The effect of heme on the conformational stability of micro-myoglobin doc
... native-like conformations.ConclusionsThe comparison of apo- and holo-lMb by MD simu-lations reveals a dramatic effect of the heme cofactor,which is seen to stabilize native Mb-like folded confor-mations ... affectsprotein conformation and dynamics; and (c) to probethe transitions of each individual helix during the ini-tial step of thermal unfolding.Results and DiscussionApo-lMbSnapshots of the MD ... pocket beforemaking contact with the cofactor [13]. For compari-son, MD simulations were also performed on ‘heme-excised’ apo-Mb, under the same conditions and initial assumptions as for apo-lMb....
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Báo cáo khoa học: The effect of pH on the initial rate kinetics of the dimeric biliverdin-IXa reductase from the cyanobacterium Synechocystis PCC6803 pptx
... first few sec-onds of the reaction under conditions when at least90% of the activity was retained; however, these areclearly not ideal conditions. With NADH as cofactor,the pK on the ‘less ... the effect of pH on the kinetic parameters of a two-substrateenzyme, one substrate is held at 10 · Km(91% saturat-ing) and the variation of initial rate with the concen-tration of the second ... the NADPH concentration constant at nonsat-urating (10 lm) and saturating (1 mm) levels of NADPH and varying the biliverdin concentration inthe range 0.5–10 lm. A concentration of 1 mm wasused...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
... experiment, conclusions regardingthe effects of the additives on the chaperone activity of aB-crystallin were drawn based on the measured percentageprotection when both the additive and chaperone werepresent ... Inaddition, for each assay we used concentrations of aB-crystallin that only partially inhibited aggregation of the target protein in order to enable the effects of the compounds on the chaperone activity ... (amorphous) aggregation systemsReduction-induced aggregation of a-lactalbuminUpon addition of 1,4-dithiothreitol (DTT), aggregation and precipitation of a-lactalbumin commenced after25 min and reached...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
... different effects on the activity of RTA. Whereas, one had no effect on the activity of the enzyme (R258A), others reduced activity by one(R213A, R258D, R48A) or by two (R213D) orders of N122AY80N122AY80Fig. ... expression levels and, as such, these mutantscould not be purified. To assess the effect of substitutionsmade at each of the arginyl residues on catalytic activity of RTA, the ability of each of ... addition of 100 lLof2· Kirby buffer [25] and 80 lLofH2O, and rRNA was obtained by precipitation after two phenol–chloroform extractions. Four micrograms of rRNA weretreatedwith20lL of acetic-aniline...
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Tài liệu Báo cáo khoa học: "The Effect of Pitch Accenting on Pronoun Referent Resolution" ppt
... attentional salience. One determiner of whether attentional or propositional effects are dominant is the type of information provided by the accented constituent. Because nonpronominals contribute ... shifts. When intonation and centering collide My synthesis of the claims in PH90 and GJW89 pro- duces an attentional interpretation of pitch accents, modeled by operations on Cf, and derived for ... focus of attention. Intonational theories would be similarly hard pressed, but on grounds of information quality and efficient use of limited resources. Given the serial and ephemeral nature of...
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Báo cáo khoa học: Dual modulation of prothrombin activation by the cyclopentapeptide plactin pptx
... plactin dually modulates prothrombinactivation, depending on the conditions of catalysis byXa. Under conditions where membrane-associated Xaformation is restricted, plactin enhances the formation of ... (B) and 0.5 nM in the other panels. Inset shows the effect of plactin D on factor Xa activity in each con-dition. The Xa concentration was 0.5 nM for all incubations and the Va concentration ... these conditions (Fig. 6B). Therefore, it was likely thatplactin–prothrombin binding altered the conformation of prothrombin and resulted in dual modulation of prothrombin activation, depending on...
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Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
... ligand under alkaline conditions and in the presence of GdmHCl is different. Under alkaline conditions release of the axial ligand is postulated to arise from thedeprotonation of an ionizable ... amounts of guanidinium hydrochloride (GdmHCl) the proteinunfolds resulting in the perturbation of a number of electronic absorption bands and the appearance in thespectrum of new ones. One of these, ... interactions (Fig. 4D). Effect of the M100K mutation on the stability of the native stateThe effect on protein stability upon introduction of aLys ligand to the heme-iron has been assessed for theM100K...
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Báo cáo khoa học: Protective effect of dietary curcumin and capsaicin on induced oxidation of low-density lipoprotein, iron-induced hepatotoxicity and carrageenan-induced inflammation in experimental rats ppt
... combination of curcumin and capsaicin. Thus, the protective effect of the combination of dietary curcumin and capsaicin on LDL oxidation both in vivo and in vitro was greaterthan that of the individual ... and conjugated dienes [51]. In experimental animals, ironoverload can be effected by intraperitoneal injection of iron salts [51].Effects of dietary curcumin, capsaicin and theircombination on ... Dietarycurcumin and dietary capsaicin significantly inhibitedthe oxidation of LDL, as indicated by TBARS valueswhich were 71 and 62% of control rats. Extent of iron-induced oxidation of LDL was considerably...
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Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt
... theregion of DNA strand breaks, and consequent forma-tion of MRN foci in cells exposed to IR, led to thehypothesis that the MRN complex functions in thesensing of DNA strand breakage and in ... theimportance of DNA strand breaks in ATM activationin vitro, suggested the possibility that the status of ATM or cofactors might determine the effect of DNAstrand ends on ATM activity. The situation ... presence of Nbs1 and its phosphorylation by ATM are requiredfor stimulation of ATM activity by the MRN complex.In contrast to the lack of a requirement of DNAstrand breaks for ATM activation in...
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