Báo cáo khoa học: Dual effect of echinomycin on hypoxia-inducible factor-1 activity under normoxic and hypoxic conditions docx

... has a dual effect on HIF-1 activity under normoxic and hypoxic conditions, demonstrating that this molecule cannot be used in cancer treatment. In the context of cancer treatment, the use of this ... basal and PMA-stimulated c-myc transcriptional activity (Fig. 2C). AP-1 activity was also low in unstimulated HepG2 cells. PMA enhanced this activity and, under n...

Ngày tải lên: 16/03/2014, 05:20

... native-like conformations. Conclusions The comparison of apo- and holo-lMb by MD simu- lations reveals a dramatic effect of the heme cofactor, which is seen to stabilize native Mb-like folded confor- mations ... affects protein conformation and dynamics; and (c) to probe the transitions of each individual helix during the ini- tial step of thermal unfolding. Results and Disc...

Ngày tải lên: 16/03/2014, 06:20

... first few sec- onds of the reaction under conditions when at least 90% of the activity was retained; however, these are clearly not ideal conditions. With NADH as cofactor, the pK on the ‘less ... the effect of pH on the kinetic parameters of a two-substrate enzyme, one substrate is held at 10 · K m (91% saturat- ing) and the variation of initial rate with the concen-...

Ngày tải lên: 23/03/2014, 05:22

... experiment, conclusions regarding the effects of the additives on the chaperone activity of aB-crystallin were drawn based on the measured percentage protection when both the additive and chaperone were present ... In addition, for each assay we used concentrations of aB-crystallin that only partially inhibited aggregation of the target protein in order to enable the effects...

Ngày tải lên: 18/02/2014, 16:20

... different effects on the activity of RTA. Whereas, one had no effect on the activity of the enzyme (R258A), others reduced activity by one (R213A, R258D, R48A) or by two (R213D) orders of N122A Y80 N122A Y80 Fig. ... expression levels and, as such, these mutants could not be purified. To assess the effect of substitutions made at each of the arginyl residues on catal...

Ngày tải lên: 19/02/2014, 12:20

... attentional salience. One determiner of whether attentional or propositional effects are dominant is the type of information provided by the accented constituent. Because nonpronominals contribute ... shifts. When intonation and centering collide My synthesis of the claims in PH90 and GJW89 pro- duces an attentional interpretation of pitch accents, modeled by operations o...

Ngày tải lên: 20/02/2014, 22:20

... plactin dually modulates prothrombin activation, depending on the conditions of catalysis by Xa. Under conditions where membrane-associated Xa formation is restricted, plactin enhances the formation of ... (B) and 0.5 nM in the other panels. Inset shows the effect of plactin D on factor Xa activity in each con- dition. The Xa concentration was 0.5 n M for all incubations...

Ngày tải lên: 07/03/2014, 00:20

... ligand under alkaline conditions and in the presence of GdmHCl is different. Under alkaline conditions release of the axial ligand is postulated to arise from the deprotonation of an ionizable ... amounts of guanidinium hydrochloride (GdmHCl) the protein unfolds resulting in the perturbation of a number of electronic absorption bands and the appearance in the spectrum...

Ngày tải lên: 07/03/2014, 17:20

... combination of curcumin and capsaicin. Thus, the protective effect of the combination of dietary curcumin and capsaicin on LDL oxidation both in vivo and in vitro was greater than that of the individual ... and conjugated dienes [51]. In experimental animals, iron overload can be effected by intraperitoneal injection of iron salts [51]. Effects of dietary curcumin, cap...

Ngày tải lên: 08/03/2014, 08:20

... the region of DNA strand breaks, and consequent forma- tion of MRN foci in cells exposed to IR, led to the hypothesis that the MRN complex functions in the sensing of DNA strand breakage and in ... the importance of DNA strand breaks in ATM activation in vitro, suggested the possibility that the status of ATM or cofactors might determine the effect of DNA strand ends on AT...

Ngày tải lên: 16/03/2014, 13:20