Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx

... Journal compilation ª 2007 FEBS 5415 The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization Caroline Heath 1 , ... catalyzes the decarboxylation of the branched-chain 2-oxoacids and pyruvate [14]. In the current paper, we report the clon- ing and expressio...

Ngày tải lên: 16/03/2014, 05:20

... [12], and thus the role of the corresponding part of the gene is also unknown. These g ene f usions togeth er with single-copy genes of APXs and CCPs are ideal for investigating the evolution of ... occur, i.e. the CCP line and the KatG fusion line, as is the c ase for Neuro spora crassa [47]. Evolution by the birth -and- death process in class I of the super...

Ngày tải lên: 19/02/2014, 16:20

... treatment abolished the internalization of the GFP-fused Tat protein but did not alter the uptake of the Tat CPP. These biochemical evidences con®rmed a pathway for the e ntry of the Tat peptide unrelated to the ... peptide (100 n M ) and increasing doses of unlabeled Tat peptide (u p to 100 l M ) only led to a slight reduction of the intracellular signal (...

Ngày tải lên: 21/02/2014, 03:20

... acyl-CoA synthetase proteins (mSA, mMACS1, mKS, and mKS 2), mouse acetyl- CoA synthetase 1 (mAceCS 1), and O-MACS proteins from rat (r), mouse (m) and human (h). O-MACS belongs to the subtree of the MACS ... (shaded). (C) Genomic organization of the mouse o-macs and other MACS family genes. They are linked in tan- dem to the 7F1 region of the mouse chromo- some 7....

Ngày tải lên: 08/03/2014, 02:20

... by the frequency of A. Subsequently, it was changed to the frequency of A followed by B divided by the product of the frequency of A and the frequency of B (Marshall, 1983: l~w~ff )) . Error ... in the light of the enormous variety and complexity of ordinary sentences in the corpus, and partly to create a databank of manually parsed samples of...

Ngày tải lên: 08/03/2014, 18:20

... s )1 ; Q116H, 0.0068 s )1 and Q116N 0.011 s )1 . In the cases of the di-zinc species (i.e. the wild-type and Q116H), these rather similar apparent k i values might correspond to the loss of the ... correspond to the mass of the N-termi- nal peptide (1299 kDa) plus 298 Da, a value that in turn corresponds to the mass of the last three amino acids of...

Ngày tải lên: 14/03/2014, 23:20

... acetate buffer (50 m M,pH5)at20gÆL )1 Avicel with the addition of b-gluco- sidase (15 kUÆL )1 ) and cellulases (24 mLÆL )1 , 3.4 gÆL )1 total protein)]. The results shown are the average of duplicates. Fig. ... differed in the analytical methods employed (X-ray diffraction versus solid state 13 C-NMR), the nature of the sub- strate used (complex lignoce...

Ngày tải lên: 15/03/2014, 10:20

... to the purine-rich strand of the CT element from the human c-myc promoter, opposite to hnRNP K that binds to the pyrimidine- rich strand. These interactions determine the formation of an open complex ... 6) and, furthermore, the inhibi- tion was proportional to the amount of H-89 used (Fig. S 5). Because most protein kinase C (PKC) iso- types may be inhibited by H-...

Ngày tải lên: 16/03/2014, 12:20

... University of Wu ¨ rzburg, Germany; 2 Department of Anatomy, University of Go ¨ ttingen, Germany; 3 Division of Cellular Biochemistry, the Netherlands Cancer Institute, Amsterdam, the Netherlands; 4 JRG ... multiple sections is seen on the left for each panel to visualize the morphology of the cells. The middle row shows staining of Smad3 and on the right an overla...

Ngày tải lên: 16/03/2014, 16:20

... (SEY621 0) and ers1D cells (XGY5 1) expressing either the human CTNS cDNA or the ERS1 gene under the control of the ERS1 promoter, were streaked on YPAD plates in the presence or absence of 50 lgÆmL )1 hygB ... to determine the precise mechanism of suppression of ers1D by MEH1, and the role of Meh1 and Gtr1 in regulating vacuolar function. The results...

Ngày tải lên: 16/03/2014, 18:20