Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx
Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx
... Journal compilation ª 2007 FEBS 5415 The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization Caroline Heath1, ... catalyzes the decarboxylation of the branched-chain 2-oxoacids and pyruvate [14]. In the current paper, we report the clon-ing and expression of the E2 and E3 genes of the sameoperon from T. acidophilum, ... lmo1Æmin )1 Æmg )1 (E 2) [17].Relative molecular mass of the E2 core and the assembled complex Analytical centrifugation and DLS were used to deter-mine the Mr of both the E2 core and the assembled complex. E2...
- 10
- 338
- 0
Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx
... [12], and thus the role of the corresponding part of the gene is alsounknown. These g ene f usions togeth er with single-copygenes of APXs and CCPs are ideal for investigating the evolution of ... occur, i.e. the CCP line and the KatG fusion line, as is the c ase for Neuro spora crassa[47].Evolution by the birth -and- death process in class I of the superfamily of bacterial, fungal, and plant ... supported the role of the catalytic triad by affecting the typical reactivity. The level of decrease in the peroxidase activity (in contrast withcatalase activity) correlated with the ability o f the...
- 13
- 512
- 0
Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... treatmentabolished the internalization of the GFP-fused Tat proteinbut did not alter the uptake of the Tat CPP. Thesebiochemical evidences con®rmed a pathway for the e ntry of the Tat peptide unrelated to the ... peptide (100 nM ) and increasing doses of unlabeled Tat peptide (u p to100 lM ) only led to a slight reduction of the intracellularsignal (data not shown). Whether there is saturation of intracellular ... [21]. Theseconsiderations indirectly reinforce the argument that the basic domain should be exposed at the surface of the Tat-containing recombinant proteins and call other reasonsto explain the...
- 8
- 485
- 0
Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
... acyl-CoA synthetase proteins (mSA,mMACS1, mKS, and mKS 2), mouse acetyl-CoA synthetase 1 (mAceCS 1), and O-MACSproteins from rat (r), mouse (m) and human(h). O-MACS belongs to the subtree of the MACS ... (shaded). (C) Genomicorganization of the mouse o-macs and otherMACS family genes. They are linked in tan-dem to the 7F1 region of the mouse chromo-some 7. The o-macs gene is composed of 14exons ... dorso-medial part of the OP and OE, whereas the NeuroD and NCAM transcripts are foundin the entire OP and OE areas. The o-macstranscripts are found in all cell layers of the OPandOEincontrasttotheNCAM...
- 10
- 393
- 0
Báo cáo khoa học: "GRAMMATICAL AN ALYSIS BY COMPUTER OF THE LANCASTER OSLO/BERGEN (LOB) CORPUS OF BRITISH ENGLISH TEXTS." potx
... by the frequency of A. Subsequently, it was changed to the frequency of A followed by B divided by the product of the frequency of A and the frequency of B (Marshall, 1983: l~w~ff )) . Error ... in the light of the enormous variety and complexity of ordinary sentences in the corpus, and partly to create a databank of manually parsed samples of the LOB corpus, for the purposes of ... for the initial upper case form (when not acting as the standard beginning -of- sentence marker) and the other for the initial lower case form. The probability of tags being selected from the...
- 6
- 409
- 0
Báo cáo khoa học: Broad antibiotic resistance profile of the subclass B3 metallo-b-lactamase GOB-1, a di-zinc enzyme potx
... s )1 ; Q116H, 0.0068 s )1 and Q116N 0.011 s )1 .In the cases of the di-zinc species (i.e. the wild-type and Q116H), these rather similar apparent kivaluesmight correspond to the loss of the ... correspond to the mass of the N-termi-nal peptide (1299 kDa) plus 298 Da, a value that in turncorresponds to the mass of the last three amino acids of the signal peptide, LNA. Another of the unidentifiedpeptides, ... ne-Poulenc(Paris, France). Chloramphenicol, cefoxitin (De260= )6 600 m )1 Æcm )1 ), cephalothin (De273= )6 300 m )1 Æcm )1 ) and EDTA were purchased from Sigma (St Louis, MO,USA) and nitrocefin (De482=...
- 12
- 406
- 0
Báo cáo khoa học: Cellulose crystallinity – a key predictor of the enzymatic hydrolysis rate pptx
... acetatebuffer (50 mM,pH5)at20gÆL )1 Avicel with the addition of b-gluco-sidase (15 kUÆL )1 ) and cellulases (24 mLÆL )1 , 3.4 gÆL )1 totalprotein)]. The results shown are the average of duplicates.Fig. ... differedin the analytical methods employed (X-ray diffractionversus solid state13C-NMR), the nature of the sub-strate used (complex lignocellulosics versus pure cellu-lose) and the source of the ... diffraction patterns of untreated Avicel and partiallyconverted cellulose in the range 10–40° (2h). x-axis: Bragg angle(2h). The reflection of face (02 1) of the crystal (centered around21 ) is visible...
- 12
- 554
- 0
Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... to the purine-rich strand of the CT element from the human c-myc promoter,opposite to hnRNP K that binds to the pyrimidine-rich strand. These interactions determine the formation of an open complex ... 6) and, furthermore, the inhibi-tion was proportional to the amount of H-89 used(Fig. S 5). Because most protein kinase C (PKC) iso-types may be inhibited by H-89, we analysed the effect of ... (pGST-CNBPWT and pGST-CNBPS158A ). Regardless of the evaluated CNBPform or the nature of the analysed probe, band-shiftintensity increased along with the protein increasingamount. Nevertheless,...
- 13
- 500
- 0
Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc
... University of Wu¨rzburg, Germany;2Department of Anatomy, University of Go¨ttingen, Germany;3Division of Cellular Biochemistry, the Netherlands Cancer Institute, Amsterdam, the Netherlands;4JRG ... multiple sections is seen on the left for each panel to visualize the morphology of the cells. The middle row showsstaining of Smad3 and on the right an overlay of Smad3 and Hoechst staining is seen.Ó ... with10% (v/v) horse serum, 5% (v/v) fetal bovine serum and antibiotics2(penicillin, 100 UÆmL )1 and streptomycin,100 lgÆmL )1 ). Transient transfection of PC12 cells wasperformed using LipofectAMINETM(Life...
- 12
- 539
- 0
Báo cáo khoa học: ERS1 encodes a functional homologue of the human lysosomal cystine transporter pptx
... (SEY621 0) and ers1D cells (XGY5 1) expressing either the human CTNS cDNA or the ERS1 gene under the control of the ERS1 promoter, were streaked on YPAD plates in the presence orabsence of 50 lgÆmL )1 hygB ... todetermine the precise mechanism of suppression of ers1D by MEH1, and the role of Meh1 and Gtr1 inregulating vacuolar function. The results we have pre-sented validate the utility of yeast as ... remove the sixth and seventh membrane spanning domains. (B) The parental wild type (SEY621 0) and ers1D (XGY5 1) strains expres-sing the indicated wild type or mutant alleles of human CTNS under the...
- 15
- 378
- 0
Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018chuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roTranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ