Báo cáo khoa học: Post-translational modifications in the active site region of methyl-coenzyme M reductase from methanogenic and methanotrophic archaea potx

... modifications; in the case of the Methanopyrus kandleri enzyme, it was not. Attempts to obtain diffracting crystals of MCR II from Methano- thermobacter marburgensis and of the MCR from the other ... methano- trophic archaea of the ANME-1 cluster. The numbering is that for the a-subunit of MCR I from Methanothermobacter marburgensis. The amino acids, whic...

Ngày tải lên: 16/03/2014, 05:20

... was incubated with liposomes con- taining atRE (A), liposomes containing atRE in the presence of 1m M bipyridine (B) and liposomes containing atRE, in the presence of 1 m M bipyridine and 6 mM ... However, immunohistochemistry detected RPE65c in the inner retina, including Mu ¨ ller cells, but not in the RPE. Furthermore, double-immuno- staining of dissociated ret...

Ngày tải lên: 14/02/2014, 14:20

... similar to the outer membrane proteins Mmm1 and Mmm2 [37]. The DAS predictor suggests Ymr110c would have a single transmembrane segment, from residues 134–152, which would anchor it in the mitochondrial ... mitochondrial outer mem- brane in yeast is integral in the membrane and most of the integral outer membrane proteins behave as if they have a-helical transmembrane...

Ngày tải lên: 19/02/2014, 07:20

... Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps. Residues present in the flexible region of the C-terminal extension of each of the eight ... located in the N-terminal domain, the comparable overall trypto- phan exposure in the mutants compared with the wild-type protein indicates that th...

Ngày tải lên: 07/03/2014, 04:20

... subsites in the polysaccharide binding site of the TSbH domain of HylP2. The positions of ligands are indicated at the substrate binding groove. (B) The view from the top shows the subsites of ascorbic ... concentrations in the range 0.2–8 mm [15], which are within the range of the IC 50 of ascorbic acid against HylP2. For the first time, the present...

Ngày tải lên: 23/03/2014, 04:21

... protein modifications often involves multiple gene products and sets of metabolites, making these events costly for a cell in terms of substrate and energy requirements. On the other hand, modifications ... A -site to the P -site and of the previously loaded tRNA from the P -site to the E(xit) -site. These steps are well conserved between organisms and the e...

Ngày tải lên: 28/03/2014, 22:20

... (FL-AA); Sigma, St Louis, MO] containing luciferin and luciferase. The reaction of the luciferin ⁄ luciferase mixture, energy donor (ATP) and oxygen results in the emission of light. The intensity of the ... with modifications. Isolated mito- chondria were added to the electrode chamber containing incubation medium (25 mm Tris ⁄ HCl, 250 mm sucrose, 8.5 mm KH 2 PO 4 ,...

Ngày tải lên: 18/02/2014, 16:20

... helices of the c ring, respectively. The C-terminal helices show a clear handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as ... that form two covalently linked hairpins in the membrane [10]. Importantly, the ion-binding site is not conserved in hairpin one. If one assumes the same nu...

Ngày tải lên: 18/02/2014, 17:20

... Follistatin, Chordin and Noggin [12]. These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibit- ing these signaling molecules from binding to their receptors ... by morpholino injections into embryos result in mild dorsalizations, and of these three BMP proteins, BMP4 seems to be the most effective [2]. Members of the zebrafi...

Ngày tải lên: 19/02/2014, 00:20

... and the one following/ the targeted word and the two preceding/ the targeted word and the two fol- lowing), and the first and last letters of the word. 3.4 Use of machine learning model The ... categories. M ethod C * there is telephone and the books on the desk. Eb: The word in the beginning of the part which should be replaced. Ee...

Ngày tải lên: 20/02/2014, 16:20