Báo cáo khoa học: Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica doc

... (2007) 4922–4940 ª 2007 The Authors Journal compilation ª 2007 FEBS Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica Emma Saavedra 1 , Alvaro Marı ´n-Herna ´ ndez 1 , Rusely ... experi- mentally determined. In the kinetic model described in the present work, the in uence of using K eq in the rate equations was eliminated by introducing the...

Ngày tải lên: 16/03/2014, 05:20

... on unknown histories. 1 Introduction One of the challenges in statistical language mod- eling are words that appear in the recognition task at hand, but not in the training set, so called out- of-vocabulary ... classes to handling OOV words in language modeling for English. Previous work on morphological classes in English has not been able to show noticeable improvements in per...

Ngày tải lên: 30/03/2014, 21:20

... forward reaction. In this regard, it is well documented that Glc6P is a potent inhibitor of hexokinase-I, hexo- kinase-II and hexokinase-III [37]. In consequence, the increased hexokinase activity ... original intracellular concentration. Determination of glycolytic flux in liver and tumor cells Cells (15 mg proteinÆmL )1 ) were incubated in 3 mL Krebs– Ringer medium with orbital stirr...

Ngày tải lên: 30/03/2014, 11:20

... site of serotonin cannot be inferred from these results. Although serotonin can bind to the active site binding locus (W82), binding to PAS cannot be ruled out. If serotonin binds only to W82, ... from tyramine binding to PAS. Such qualitatively opposite effects can be tenta- tively interpreted in terms of allosteric inhibition ⁄ acti- vation: the binding of tyramine to PAS induces a co...

Ngày tải lên: 18/02/2014, 17:20

... 3¢ splice site can play a role in determining the binding regis- ter of reaction substrates. Biochemistry 44, 1067–1077. P. P. Dotson II et al. Kinetics of the trans excision-splicing reaction FEBS ... reaction is 4.1Æmin )1 (Fig. 2), with a typical end point of 70–80%. Over the period of 15–60 min, this end point does not change, indicating that either an internal equilibrium Table 1. K...

Ngày tải lên: 18/02/2014, 18:20

... self-standing structure protruding from the interface between the DNA binding and the active site subdomains, we man- aged to model the entire domain without it. Another significant finding concerns ... 51–55. Supporting information The following supporting information is available: Fig. S1. The in uence of (A) divalent cations, (B) pH and (C) temperature on Rep245 polymerase activity. This su...

Ngày tải lên: 18/02/2014, 18:20

... were preincubated in the pres- ence (s) and absence (d) of 0.75 l M ChlH in syringe 1. Syringe 2 contained only MgD. (A) MgDME evolution was followed by integ- rating the peaks at 12.3 min on the ... of this reaction intermediate, resulting in a reduction in the lag phase of product accumulation, and an increase in initial rate of product evolution. Furthermore, the presence of ChlH...

Ngày tải lên: 20/02/2014, 02:21

... minor, implying rotations of domains of just a few degrees (e.g. 7° interdomain rotations upon tRNA binding to the Tt-GluRS and local limited rearrangements in the active site) [5]. Interestingly, ... provide a rationale for the discriminating behaviour of Mt-GluRS in E. coli. Studies on Tt-GluRS indicated that discriminating and nondiscriminating GluRS can be distinguished on the basis...

Ngày tải lên: 07/03/2014, 03:20

... designed against methionine gamma- lyase-containing pathogens, has efficacy in vitro and in vivo against Trichomonas vaginalis. Antimicrob Agents Chemother 45 , 1743–1745. 27 Inoue H, Inagaki K, ... but were minor contaminants in the recombinant protein preparations. To examine whether irrelevant proteins can also serve as scavengers of carbonothionic difluoride produced from TFM by K...

Ngày tải lên: 07/03/2014, 05:20

... Canters G (2000) Kinetic and paramagnetic NMR investigations of the inhibition of Streptomyces antibioticus tyrosinase. J Mol Catal 8B, 27–35. 38 Salvato B & Beltramini M (1990) Hemocyanins: ... in l:g 2 -g 2 coordination at type 3 copper centers [5,6]. Type 3 copper centers cannot only reversibly bind oxygen, as in hemocyanins, but can also activate and metabolize oxygen in enz...

Ngày tải lên: 07/03/2014, 05:20