Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTC ACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢). For PCR, the reaction mix consisted of 1 · Taq buffer contain- ing 1.5 ... dsRNA; or (b) small interference (si )RNA. The longer dsRNA may generate a large population of siRNA (with 21–23 nucleotides), and the use of longer dsRNA may be advantageous...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: Combined use of selective inhibitors and fluorogenic substrates to study the specificity of somatic wild-type angiotensin-converting enzyme docx
... Mca-Ala is processed by the N-domain and C-domain varies according to the ACE species. For rat and human enzymes, estimation of IC 50 values for the binding of the inhibitor to the N-domain and ... Mca-Ala substrate Table 1. Kinetic parameters for the hydrolysis of the Mca-Ala substrate by rat, mouse and human somatic ACE. Kinetic parameters k cat and K m wer...
Ngày tải lên: 16/03/2014, 13:20
Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
... medicin), the Cancer Founda- tion, the European Union FP7 grant EURO-PADnet, and the Torsten and Ragnar So ¨ derberg Foundation. Rani Faryal was a recipient of a Postdoctoral Fellow- ship from the ... linked to the development and main- tenance of hematological malignancies [67]. Moreover, as a result of chromosomal translocation, a chimera, consisting of...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... methylation analysis of milk oligosaccharides. PMAA, partially methylated alditol acetate. Molar ratios are relative to 1,5-di-O-acetyl-2,3,4,6-tetra-O-methylgalactitol. –, Not detected. PMAA Linkage Molar ... was delivered by a Harvard syringe pump (Harvard Apparatus, Holliston, MA, USA) at a flow rate of 10 lLÆmin )1 . Alternatively, 0.5–1 lL sample solution was placed in a capil...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf
... of these sequences are derived from the Mcp [11], and the Fab-7 [12] regions. Both contain a boundary and at least one PRE, and are able to medi- ate long-distance regulatory interactions via ... both iab-5 and iab-6 are active, and iab-7 and iab-8 are silenced, but only iab-6 drives the expression of Abd-B. Similarly, although three different Abd-B cis-regulatory...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf
... crassa, Candida tropicalis, Dictyostelium discoideum, Medicago varia (alfalfa), Arabidopsis t haliana, Oryza sativa (rice), Caenorhabditis elegans, Drosophila m elanogaster, Xenopus laevis, a ... polypeptides to the beads, and suboptimal binding in the absence of cotranslation of the A and C subunits. The smallest N-terminal fragmen t of B5 6a tha t interacted with GS...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Ligand binding promotes prion protein aggregation – role of the octapeptide repeats potx
... molecular mass of 2000 kDa was used as a marker in the gradient. Statistical analysis A two-way ANOVA program was used to determine the P-value between various groups. P > 0.05 is considered to be ... identical conditions, all the immuno- reactivity remained on the top of the gradient. Fig. 2. Characterization of the heparin enhanced aggregation of rPrP. (A) Com...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... endo-b-1, 4-glucanase and b-glucosidase, have been detected in the gut of P. hilaris larvae and adults [8]. To clarify further the cellulase activity in P. hilaris,we purified, characterized and obtained the ... PCR and sequencing A QuickPrepÒ Micro mRNA Purification Kit (Amersham Bioscience) was used for isolation of mRNA from P. hilaris larval midguts. First-strand cDN...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: "PCFGs, Topic Models, Adaptor Grammars and Learning Topical Collocations and the Structure of Proper Names" ppt
... all of the proper names (i.e., phrases of category NNP and NNPS) in the Penn WSJ treebank and used them as the training cor- pora for the adaptor grammar just described. The adaptor grammar inference ... and use the adaptor gram- mar machinery to learn these collocations. Inspired by the grammar presented in Elsner et al. (2009), our adaptor grammar is as follows,...
Ngày tải lên: 23/03/2014, 16:20
Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx
... factors nuclear factor for activated T cells (NFAT), nuclear factor kappa-light chain enhancer of activated B cells (NFjB) and activator protein- 1 [4]. A number of studies have examined the Keywords development; ... Ca 2+ , and defective activation of extracellular signal-regulated kinase ⁄ mitogen-activated protein kinase (ERK ⁄ MAPK), with resultant reduction in the acti...
Ngày tải lên: 28/03/2014, 22:21