Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt

... primers(forward, 5¢-TAATACGACTCACTATAGGTACTATGTATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTCACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢). ForPCR, the reaction mix consisted of 1 · Taq buffer contain-ing 1.5 ... dsRNA; or (b) small interference (si )RNA. The longer dsRNA may generate a large population of siRNA (with 21–23 nucleotides), and the use of longer dsRNA may be advantageous over siRNA. Inthis study, ... neuropeptides from the shrimp Metapenaeus ensis have been cloned, the functions of most of theseneuropeptides remained putative. In this article, we describe the use of recombinant protein and an RNA interference...

Báo cáo khoa học: Combined use of selective inhibitors and ﬂuorogenic substrates to study the speciﬁcity of somatic wild-type angiotensin-converting enzyme docx

... Mca-Ala is processed by the N-domain and C-domain varies according to the ACE species. For rat and human enzymes, estimation of IC50values for the binding of the inhibitor to the N-domain and ... Mca-Ala substrateTable 1. Kinetic parameters for the hydrolysis of the Mca-Ala substrate by rat, mouse and human somatic ACE. Kinetic parameters kcat and Kmwere obtained by inhibiting the activity ... Potency and selectivity of RXP407 and RXPA380 toward the N-domain and C-domain of human, mouse and rat somaticACE. Ki(app)values were determined from the simulations that bestreproduced the inhibition...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... medicin), the Cancer Founda-tion, the European Union FP7 grant EURO-PADnet, and the Torsten and Ragnar So¨derberg Foundation.Rani Faryal was a recipient of a Postdoctoral Fellow-ship from the ... linked to the development and main-tenance of hematological malignancies [67]. Moreover,as a result of chromosomal translocation, a chimera,consisting of the dimerizing TEL protein and SYK,was ... activation and plasma membrane localization of the fusion constructare dependent on phosphatidylinositol 3-kinase signal-ing, and that ITK–SYK phosphorylates the adapterproteins SLP-76 and...

Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

... methylation analysis of milk oligosaccharides. PMAA, partially methylatedalditol acetate. Molar ratios are relative to 1,5-di-O-acetyl-2,3,4,6-tetra-O-methylgalactitol. –, Not detected.PMAA LinkageMolar ... wasdelivered by a Harvard syringe pump (Harvard Apparatus,Holliston, MA, USA) at a ﬂow rate of 10 lLÆmin)1.Alternatively, 0.5–1 lL sample solution was placed in a capillary needle for the nanospray experiment.NMR ... serve ascell differentiation and tumour antigens [5]. Milk oligosac-charides are considered to play a part in the inhibition of bacterial adhesion to epithelial surfaces, as they are able to mimic...

Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf

... of these sequences are derived from the Mcp [11], and the Fab-7 [12] regions. Both contain a boundary and at least one PRE, and are able to medi-ate long-distance regulatory interactions via ... bothiab-5 and iab-6 are active, and iab-7 and iab-8 aresilenced, but only iab-6 drives the expression of Abd-B.Similarly, although three different Abd-B cis-regulatorydomains are active in A7 , the ... pushed aside. The organization and functioning of the Abd-B gene suggest that the looping model is alsoapplicable to the Abd-B regulatory unit. In A6 , forexample, enhancers in iab-6 have to reach...

Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf

... crassa,Candida tropicalis, Dictyostelium discoideum, Medicagovaria (alfalfa), Arabidopsis t haliana, Oryza sativa (rice),Caenorhabditis elegans, Drosophila m elanogaster, Xenopuslaevis, a ... polypeptides to the beads, and suboptimal binding in the absence of cotranslation of the A and C subunits. The smallest N-terminal fragmen t of B5 6a tha t interacted withGST -A encompasses r esidues ... phosphatase; PP 2A; subunitinteractions; phosphorylation. Protein phosphatase 2A (PP 2A) is an abundant cellularserine/threonine-speci®c phosphatase that regulates a sig-ni®cant array of cellular...

Báo cáo khoa học: Ligand binding promotes prion protein aggregation – role of the octapeptide repeats potx

... molecular mass of 2000 kDa was used as a marker in the gradient.Statistical analysis A two-way ANOVA program was used to determine the P-value between various groups. P > 0.05 is considered to be ... identical conditions, all the immuno-reactivity remained on the top of the gradient.Fig. 2. Characterization of the heparin enhanced aggregation of rPrP. (A) Comparison of the aggregation of rPrP ... variousamounts of NaCl ⁄ Pi and added to individual ELISAwells, which had been pre-coated with an anti-PrPmAb, 11G5, to capture the rPrP. An avidin-conju-gated enzyme was then added to the...

Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... endo-b-1,4-glucanase and b-glucosidase, have been detected in the gut of P. hilaris larvae and adults [8]. To clarify further the cellulase activity in P. hilaris,wepuriﬁed, characterized and obtained the ... PCR and sequencing A QuickPrepÒ Micro mRNA Puriﬁcation Kit (AmershamBioscience) was used for isolation of mRNA from P. hilarislarval midguts. First-strand cDNA synthesis from the isolated mRNA ... Horiba, Kyoto, Japan).Enzyme assay A carboxymethylcellulase (CMCase) assay was performedby measuring the amount of reducing sugars after incuba-tion of 100 lL 1% (w/v) CMC (standard molecular...

Báo cáo khoa học: "PCFGs, Topic Models, Adaptor Grammars and Learning Topical Collocations and the Structure of Proper Names" ppt

... all of the proper names (i.e.,phrases of category NNP and NNPS) in the PennWSJ treebank and used them as the training cor-pora for the adaptor grammar just described. The adaptor grammar inference ... and use the adaptor gram-mar machinery to learn these collocations.Inspired by the grammar presented in Elsneret al. (2009), our adaptor grammar is as follows,where adapted nonterminals are ... tend to have the same topic.5 Adaptor GrammarsNon-parametric Bayesian inference, where the in-ference task involves learning not just the values of a ﬁnite vector of parameters but which parame-ters...

Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

... factors nuclear factor for activatedT cells (NFAT), nuclear factor kappa-light chainenhancer of activated B cells (NFjB) and activator protein- 1 [4]. A number of studies have examined the Keywordsdevelopment; ... Ca2+, and defective activation of extracellular signal-regulatedkinase ⁄ mitogen-activated protein kinase (ERK ⁄MAPK), with resultant reduction in the activation of the transcription factors ... Depiction of the signaling pathway used by the TCR and modulated by Itk that results in the development of i NKT ab and cd T cells. Note that in the case of the TCR, these pathways seem to beshared...

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