Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc

... respectively, and pcitrine-N1. phPGK1–7aa–cerulean and phPGK1–5aa–cerulean were constructed using the primers 5¢-CCGGA ATTCC AATGT CGCTT TCTAA CAAGC T-3¢ and 5¢-GGCGG ATCCA TAATA TTGCT GAGAG CATCC A- 3¢, and ... coexpressed as shown in the FLIM images (Fig. 3A, C) and in Table 2. On the other hand, the lifetimes of PGK–7aa–cerulean and their intracellu- lar distribu...

Ngày tải lên: 16/02/2014, 09:20

... Orientale, Alessandria, Italy; 3 Dipartimento di Biochimica e Biologia Molecolare, Universita ` di Parma, Italy This paper reports the isolation and characterization of the regulatory moiety of the ... respectively. Kinetic constants The catalytic activity of PHR was evaluated both in the presence and in the absence of PHI. K m and k cat were determined from Ha...

Ngày tải lên: 21/02/2014, 00:20

... one shown in A) . (C) GST fusion proteins containing the C termini of a, b,andcENaC were phosphorylated by fraction 76. Autoradiogram and Coomassie blue staining are shown. Fig. 2. Binding of kinases ... was performed using polyclonal antibodies against the a and a subunits of CK2. The catalytic subunit of the enzyme could indeed be detected in fractions 7...

Ngày tải lên: 21/02/2014, 03:20

... substantia nigra and corpus striatum of patients with PD are unknown, a recent analysis indicated that the average concentration of NMSal in the substantia nigra, caudate nucleus and putamen of individuals ... l-3,4- dihydroxyphenylalanine (l-DOPA), which is the rate- limiting step in synthesis of the catecholamines DA, norepinephrine and epinephrine. TH consists...

Ngày tải lên: 07/03/2014, 05:20

... a preference (at least partial) for binding to the N-terminal b-domain [4,7]. These metal–thiolate cluster structures are unusual for metal -binding proteins and are responsible for the typical ... domains (a b-domain for the N-terminal cluster and an a- domain for the C-terminal cluster). In the absence of metals, apo-thionein (apo-T) is predominantly...

Ngày tải lên: 16/02/2014, 15:20

... and functional properties of RNase and participates in degradation of IL-1b mRNA Danuta Mizgalska 1 , Paulina We˛ grzyn 1 , Krzysztof Murzyn 2 , Aneta Kasza 1 , Aleksander Koj 1 , Jacek Jura 3 , Barbara ... pro- moter and cDNA for luciferase with or without AREs from IL-1b cDNA (p-luc )ARE and p-luc), the region flanking the AREs in IL-1b cDNA was amplified using forward...

Ngày tải lên: 18/02/2014, 14:20

... data (different from the training data) 1 . Reranking Candidate 1 Candidate 2 Candidate 3 Candidate 4 : Case element : Verb Candidate Candidate Figure 2: Selection of possible parses for reranking Many ... higher than that of the test data, probably because the syntactic constraint in this model affects parsing too much. Therefore the variance of the reranking model (Equat...

Ngày tải lên: 20/02/2014, 12:20

... synthesis and release of pro- in ammatory mediators [17]. It is now widely accepted that the latter are responsible for disruption of the capillary basal lamina, opening of the BBB and in l- tration ... Italy Therapeutic strategies aimed at reducing brain dam- age after ischemic stroke have been a major focus of academic and industrial research for the past 30...

Ngày tải lên: 07/03/2014, 03:20

... between NAT and OAB (Fig. 7C), indicating that NATA and NAT bind at the same or overlapping sites where two mole- cules of OAB also bind, and that binding of OAB at the substrate- binding site is ... substrate- binding site. Mutually exclusive binding of OAB, NAT and NATA indicates that the binding sites of these inhibi- tors are the same or overlapping....

Ngày tải lên: 07/03/2014, 05:20

... Introduction While parsers are been used for other purposes, the primary motivation for syntactic parsing is as an aid to semantic interpretation, in pursuit of broader goals of natural language understanding. ... is in turn es- sential to semantic interpretation of the kind prac- ticed in the semantic parsing (Gildea and Jurafsky, 2002) and QA (Pasca and Harabag...

Ngày tải lên: 08/03/2014, 04:22