Báo cáo khoa học: Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions ppt

... FEBS Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions Qiurong Li 1,2 , Qiang Zhang 1,2 , Chenyang Wang 1 , Ning Li 1 and Jieshou Li 1 1 Institute of ... role of lipid rafts in EPEC invasion of epithelial cells, we used confocal fluores- cence microscopy to observe the entry of the bacteria into epithelial...

Ngày tải lên: 16/03/2014, 04:20

... effect of transamination is a reduction of the degre e of 15 N-labelling in the amino a cid of interest. In thecaseof[ 15 N]Gly, much of the label appeared to have been trapped in an a mide group of ... cleavage. This was controlled by use of an ompT – E. coli host strain [BL21(DE3)recA], limitation of thetimeculturesweretreatedat42°C during i nduction of synthesis of...

Ngày tải lên: 16/03/2014, 18:20

... phos- photransferase system (PTS) in Escherichia coli is one of the best studied biochemical networks from theo- retical and experimental points of view, and has Keywords Escherichia coli; model verification; ... modeling of the central carbon metabolism of Escherichia coli. Biotech- nol Bioeng 79, 53–73. 8 Mahadevan R, Edwards JS & Doyle FJ (2002) Dynamic flux balance ana...

Ngày tải lên: 18/02/2014, 13:20

... synchronization of large numbers of stores across many cells [4,19]. Gastric smooth muscle cells and associated interstitial cells of Cajal (ICCs) form a syncytium interconnected by gap junctions. ... activity of many smooth mus- cles [1,11–13] (Fig. 1). Slow waves cause entry of Ca 2+ through opening of L-type Ca 2+ channels and contrac- tions of the smooth muscle. Cyc...

Ngày tải lên: 16/02/2014, 09:20

... mem- brane and the cells are tightly packed, which is true of neuroepithelial cells, the voltage fluctuation of the store membrane is synchronized between the cells by the AC currents through the series ... between cells in the neuroepithelium. If neuroepithelial cells were capable of generating action potentials synchronized by gap junctions (direct current electrical coup...

Ngày tải lên: 16/02/2014, 09:20

... the perturbation of lipids, which is a result of both hydro- phobic mismatch of the C-terminal region of Ab within the lipid bilayer and the favorable interaction of the N-terminal segment of Ab with ... disrupt the integrity of the lipid membrane [11,12], another parameter of interest is the local thickness of the bilayer. We previously reported the capacity of water to p...

Ngày tải lên: 18/02/2014, 08:20

... transcription of p53 and this is critical for an antiviral defence of the host. In addition, T cells with a lack of RGS2 impair antiviral immunity [37]. In con- clusion, after activation of TLR3 by ... the involvement of different MAP kinases was determined. Use of PD98059, an inhibitor of Erk, or SB203580, an inhibitor of p38, had no effect on RGS1 or RGS2 reg- ulation aft...

Ngày tải lên: 18/02/2014, 13:20

... analyses of p140 and p48 from meiotic cells The expression profile of each subunit of DNA polymerase a-primase has been shown in mammalian somatic cells [13] and yeast [33,34]. The transcripts of both ... but also during the leptotene/zygotene stages of meiotic prophase I. Insituimmuno-staining of cells at meiotic prophase I revealed a sub population of p48 that does not co...

Ngày tải lên: 20/02/2014, 11:20

... flank lengths of 0–12 bases in KCl. We observed enhance- ment of fluorescence intensity on increasing the con- centration of the complementary strand, indicative of a greater extent of quadruplex ... 1). To analyze the effect of flanking bases on quadruplex Fig. 1. Schematic representation of the 21-mer G-rich sequence located )87 bp upstream of the transcription start site of...

Ngày tải lên: 07/03/2014, 02:20

... Lillehaug Department of Molecular Biology, University of Bergen, Norway Haemerythrin proteins comprise a family of O 2 -carrry- ing proteins mainly found in a few phyla of marine invertebrates. Members of this ... Fifteen of these spots were differentially expressed. One of the polypeptides migrated with an apparent molecular mass of 14.4 kDa and a pI value of  5.8 (Fig. 1,...

Ngày tải lên: 07/03/2014, 17:20