Báo cáo khoa học: Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions ppt
Báo cáo khoa học: Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions ppt
... FEBS Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions Qiurong Li1,2, Qiang Zhang1,2, Chenyang Wang1, Ning Li1and Jieshou Li11 Institute of ... role of lipid rafts in EPEC invasion of epithelial cells, we used confocal fluores-cence microscopy to observe the entry of the bacteria into epithelial cells, and confirmed that penetration of EPEC ... October2008)doi:10.1111/j.1742-4658.2008.06731.x Enteropathogenic Escherichia coli (EPEC) has been shown to disrupt thebarrier function of host intestinal epithelial tissues through entering tight junctions. However, the...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
... effect of transamination is a reduction of the degre e of 15N-labelling in the amino a cid of interest. Inthecaseof[15N]Gly, much of the label appeared to havebeen trapped in an a mide group of ... cleavage. This was controlled by use of anompT–E. coli host strain [BL21(DE3)recA], limitation of thetimeculturesweretreatedat42°C during i nduction of synthesis of T7 RNAP to 30 min, followed by ... directedoverproduction of the protein under control of the heat-inducible k pLpromoter. Up to 25 mg of pure T7RNAP could be obtained within a few days from 5 g of cells. This is sufficient for many hundreds of...
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Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx
... phos-photransferase system (PTS) in Escherichia coli is one of the best studied biochemical networks from theo-retical and experimental points of view, and hasKeywords Escherichia coli; model verification; ... modeling of thecentral carbon metabolism of Escherichia coli. Biotech-nol Bioeng 79, 53–73.8 Mahadevan R, Edwards JS & Doyle FJ (2002)Dynamic flux balance analysis of diauxic growth in Escherichia ... signaltransduction models in Escherichia coli. Bioinformatics24, 2044–2050.14 Kremling A, Bettenbrock K & Gilles ED (2007) Analy-sis of global control of Escherichia coli carbohydrateuptake....
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Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt
... synchronization of large numbers of storesacross many cells [4,19]. Gastric smooth muscle cells and associated interstitial cells of Cajal (ICCs) form asyncytium interconnected by gap junctions. ... activity of many smooth mus-cles [1,11–13] (Fig. 1). Slow waves cause entry of Ca2+ through opening of L-type Ca2+channels and contrac-tions of the smooth muscle. Cyclical release of Ca2+from ... by distances of meters. Thissynchronization of clock pendulums occurred through coupling between the pendulums by transmission of minute vibrations through the wall. An example of cou-pled...
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Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx
... mem-brane and the cells are tightly packed, which is true of neuroepithelial cells, the voltage fluctuation of the store membrane is synchronized between the cells by the AC currents through the series ... between cells in theneuroepithelium. If neuroepithelial cells were capable of generating actionpotentials synchronized by gap junctions (direct current electrical coupling),the influx of Ca2+ through ... betweenneuroepithelial cells and newborn RGCs, which lackgap junctions (Fig. 1D). The electrical circuits of thecurrent loop are presented in Doc. S2 of [5].The fluctuation in the membrane potential of...
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Tài liệu Báo cáo khoa học: Perturbation of membranes by the amyloid b-peptide – a molecular dynamics study pptx
... theperturbation of lipids, which is a result of both hydro-phobic mismatch of the C-terminal region of Abwithin the lipid bilayer and the favorable interaction of the N-terminal segment of Ab with ... disrupt the integrity of thelipid membrane [11,12], another parameter of interestis the local thickness of the bilayer. We previouslyreported the capacity of water to penetrate into thebilayer ... integrity of the plasma membrane,Ab promotes the increased leakage of ions, particularlycalcium, into the cell [10]. The disruption of calciumhomeostasis, and thus the promotion of neuronalexcitotoxicity,...
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Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... transcription of p53 and this is critical for anantiviral defence of the host. In addition, T cells witha lack of RGS2 impair antiviral immunity [37]. In con-clusion, after activation of TLR3 by ... theinvolvement of different MAP kinases was determined.Use of PD98059, an inhibitor of Erk, or SB203580, aninhibitor of p38, had no effect on RGS1 or RGS2 reg-ulation after 0.5 and 6 h of stimulation ... regulation of different proteins is enforced by RGS2 mRNA after12 h of stimulation. The upregulation of both RGSmRNA was found only after activation of the TLR3signalling pathway. This upregulation of...
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Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-primase complex during meiosis in Coprinus cinereus pptx
... analyses of p140 and p48from meiotic cells The expression profile of each subunit of DNA polymerasea-primase has been shown in mammalian somatic cells [13]and yeast [33,34]. The transcripts of both ... but also during the leptotene/zygotene stages of meiotic prophase I. Insituimmuno-staining of cells atmeiotic prophase I revealed a sub population of p48 thatdoes not colocalize with p140 in ... [11,12]. When quiescent cells beginto proliferate, mRNA levels of all subunits of pol a areelevated [13], as are consequent translation rates andenzyme activities [14].Upon entry into the leptotene...
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Báo cáo khoa học: Effect of flanking bases on quadruplex stability and Watson–Crick duplex competition pptx
... flanklengths of 0–12 bases in KCl. We observed enhance-ment of fluorescence intensity on increasing the con-centration of the complementary strand, indicative of agreater extent of quadruplex ... 1).To analyze the effect of flanking bases on quadruplexFig. 1. Schematic representation of the 21-mer G-rich sequencelocated )87 bp upstream of the transcription start site of the c-kitgene. The ... physiological pH of 6.0. The pres-ence of intramolecular stem–loop structures in theC-rich complementary strand leads to the existence of competition and thus hinders opening of the secondarystructures...
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Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt
... LillehaugDepartment of Molecular Biology, University of Bergen, NorwayHaemerythrin proteins comprise a family of O2-carrry-ing proteins mainly found in a few phyla of marine invertebrates. Members of this ... Fifteen of these spots were differentially expressed. One of the polypeptides migrated with an apparent molecularmass of 14.4 kDa and a pI value of 5.8 (Fig. 1,spot 15) and was only found in cells ... differentbacteria of 174 sequenced bacterial genomes.Owing to the importance of copper in the biology of M. capsulatus, it is of great interest to study proteinexpression in cells cultured at...
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