Báo cáo khoa học: Ligand binding promotes prion protein aggregation – role of the octapeptide repeats potx

... features of insertion mutant prion proteins FEBS Journal 275 (2008) 556 4–5 575 ª 2008 The Authors Journal compilation ª 2008 FEBS 5575 Ligand binding promotes prion protein aggregation – role of the octapeptide ... number of inserts [12]. We therefore investigated whether heparin, a GAG, promotes the aggregation of rPrPs, and whether the degree of...

Ngày tải lên: 16/03/2014, 04:20

... pH-dependent binding of IgG and albu- min to shFcRn, implies that antibodies were generated that recognized either of the ligand- binding sites located in the a2 domain. Thus, the antigenic proper- ties of ... measurements. Subsequently, immunization of goats with either shFcRn or the easily obtained HC gave rise to specific antibodies that inhibited the binding of b...

Ngày tải lên: 16/03/2014, 06:20

... mice [28]. The instability of DA at the neutral pH of the cytosol causes the forma- tion of toxic aminochromes, and these species are not formed when DA is protonated at the acidic pH of the storage ... counter. Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake...

Ngày tải lên: 14/02/2014, 21:20

... have therapeutic value as inhibitors of SET1 family complexes. Binding of the MLL1 Win motif to the central argi- nine -binding pocket of WDR5 raises questions about the proposed role of WDR5 in binding ... include the MLL1 CXXC domain [22], a portion of the MLL1 TAD bound to the KIX domain of the cAMP response element -binding (CREB) binding protein (CBP...

Ngày tải lên: 16/02/2014, 14:20

... remains no clear con- sensus on the role of GIF in the pathogenesis of AD, and therefore we will briefly review some of the current thoughts on the role of GIF in AD. There are numer- ous pathological ... potential role of GIF in neurofibrillary changes associated with AD. Potential role of metal -binding/ exchange properties of GIF in AD One of the primary pat...

Ngày tải lên: 16/02/2014, 15:20

... with the PIR–BPS of Grb14 and of the Grb14 SH2 domain have allowed us to propose a model for the Grb14–IR interaction. The PIR–BPS binds as a pseudosubstrate inhibitor in the substrate- binding ... inhibits the tyrosine kinase activity of the IR [7]. On the basis of the crystal structure of the tyrosine kinase domain of the IR in complex with the PIR ⁄ BP...

Ngày tải lên: 18/02/2014, 18:20

... described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The ... bonded to the e-amino group of the Lys9 side-chain, which simultaneously interacts with the main phosphate of the substrate [32]. The closer positioning of...

Ngày tải lên: 19/02/2014, 12:20

... inter- leukin-1b. The present work reviewed the role of neuroinflammatory mediators in the pathophysiology of ischemic brain damage and their poten- tial exploitation as drug targets for the treatment of cerebral ... has also emphasized the role of MMPs and their endoge- nous inhibitors (tissue inhibitor of matrix metallopro- teinases) in the regulation of neuronal...

Ngày tải lên: 07/03/2014, 03:20

... and in the range of the CV of the NADPH determination. Discussion Obvious tendencies found by characterization of the wild-type In the exponential phase of the ammonium cultures all of the PPP ... [26]. Overproduction of 6PGDH resulted in an increase of synthesis of pyr- idine nucleotide cofactors, as the total pool of these increased. Whether the synthesis...

Ngày tải lên: 07/03/2014, 17:20

... that the clearance and further metabo- lism of the allergen was altered as a result of the inflammation in the lungs of sensitized animals. Up to now there are few data available on the fate of ... probes to proteins is that the metabolism of 75 Se-labelled proteins can readily be followed through identification of newly synthesized selenoproteins, as the major endogen...

Ngày tải lên: 07/03/2014, 21:20