Báo cáo khoa học: Solution structure of the bb¢ domains of human protein disulfide isomerase docx

... mecha- nism of transphosphorylation, which implies the A B Fig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-site residues of the 20 conformers of the solu- tion structure ... second- ary structure elements. As in other ribotoxins, these A B C Fig. 2. Representation of the 3D structure of HtA in solution. (A) Superposition...

Ngày tải lên: 18/02/2014, 08:20

... [15]. The solution structure of the MBD of rat (r)MeCP2 has recently been determined [17]. The MBD adopts a wedge- shaped structure, composed of an antiparallel b-sheet on one face of the wedge ... cluster in these two regions. Know- ledge of the structure of a larger portion of MeCP2 including the transcriptional repression domain would resolve these speculat...

Ngày tải lên: 21/02/2014, 00:20

... al. Solution structure of the RICH catalytic domain FEBS Journal 274 (2007) 1600–1609 ª 2007 The Authors Journal compilation ª 2007 FEBS 1609 Solution structure of the catalytic domain of RICH protein from ... 1600–1609 ª 2007 The Authors Journal compilation ª 2007 FEBS 1601 Results Structure of the RICH catalytic domain We determined the structure of the...

Ngày tải lên: 07/03/2014, 10:20

... towards the interior of the protein. The space that in the wild-type molecule is occupied by the large hydrophobic side chain of Fig. 3. Structure ensemble of HPr(I14A). The average structure of the 10 ... for the calculations. Therefore, we recalculated the structure of the w ild-type protein on the b asis of the restraints used previously [15] wi...

Ngày tải lên: 07/03/2014, 16:20

... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the struc- tures of ... that a segment of the human Stch (hStch) protein can bind to the STI1-homologous domain (Fig. 1B) of the Chap1 (hPLIC-1) protein [24]. Therefore, we performed seque...

Ngày tải lên: 07/03/2014, 17:20

... Structure of the bb¢ domains from human PDI FEBS Journal 276 (2009) 1440–1449 ª 2009 The Authors Journal compilation ª 2009 FEBS 1449 Solution structure of the bb¢ domains of human protein disulfide isomerase Alexey ... with the crystal structure of yeast PDI-b¢ (red, Protein Data Bank entry code 2B5E). (C) Superimposition of the solution stru...

Ngày tải lên: 16/03/2014, 04:20

... the prior formation of the Zn 3 NcMT and its titration with Cu(I). The enhanced sensitivity and resolution of the 800 MHz 1 H-NMR spectral data p ermitted the 3 D structure determination of the ... family of proteins when using lower field, less sensitive 1 H-NMR spectral data. The accu- racy of the structure calculated without these constraints is, however, suppo...

Ngày tải lên: 23/03/2014, 13:20

... although the length of loop I is longer in short neurotoxins. TheNMRstructureofNTX-1isclosesttothatofthe a-cobratoxin crystal. The superposition of these two structures shows an RMSD value of 3.00 ... of the X-ray structure can be observed in the NMR structures but the length of the b-strand in loop I in solution is somewhat shorter than that of the crystal one (...

Ngày tải lên: 23/03/2014, 13:20

... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... in the immediate vicinity of residue 28 (Fig. 3B), indicating that the overall structure of Xis_wt is not affected by the substitution. The DSC study revealed that...

Ngày tải lên: 23/03/2014, 15:21

... effect. Description of the toxin structure and potential surface The characteristic NOE connectivities, reported in Fig. 3A, and the analysis of the / and w angle distribution allowed the identification of the ... )1 topology [47] of the triple-stranded antiparallel b-sheet was Fig. 3. Summary of data used in the determination of the secondary structure of c...

Ngày tải lên: 20/02/2014, 11:20