Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death potx

... FEBS Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death Paola Matarrese 1 , Elisabetta Straface 1 , Giuseppe Palumbo 2 , ... proteins Bax and Bak, caspase activation and cell death [2,3]. In addition, the physi- ological platelet agonist thrombin also induces Bid, Bax...

Ngày tải lên: 16/03/2014, 04:20

... up after 4 8–7 2 h. Revertant charac- terization was completed by sequencing their chitosanase genes. Chitosanase purification and assay Chitosanase and protein assays were performed as described ... other reagents and enzyme substrates were of analytical grade and are commer- cially available. Culture media components were obtained from Difco (Mississauga, Canada). Acknowledgement...

Ngày tải lên: 16/03/2014, 04:20

... 693 TTTTTCAGTGCAGAA-3¢; aP2 forward, 5¢-AAAGACA GCTCCTCCTCGAAGGTT-3¢; and aP2 reverse, 5¢-TGA CCAAATCCCCATTTACGC-3¢. Standard curves were gen- erated with 10-fold serial dilutions ranging from 1 ⁄ 10 to 1 ... cellular apoptosis [36]. In human lung carcinoma cells, rosiglitazone suppresses cellular proliferation via a PPARc-dependent Akt-1 signaling pathway, as well as by a PPARc-ind...

Ngày tải lên: 16/02/2014, 09:20

... -ATC ATC TCC ATC GAC TAC TCC CTG-3¢, the antisense primer 5¢-AAG AAT TCT AGA TTA ATG GTG ATG ATG GTG ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG GGG GGT-3¢ (XbaI sites underlined; His-tag in italic) ... phospholipids and that this is fol- lowed by an interaction between the apolar acyl chains of the phospholipids and side chains of particular amino acids, thus accounting for the increased c...

Ngày tải lên: 18/02/2014, 11:20

... percentage of cells that displayed a FL2-H fluorescence value higher than 2 · 10 1 was auto- matically determined and used to calculate the percentage of mitochondrial membrane potential disruption induced by ... lysates was then analyzed according to the manufacturer’s instructions. Absorbance was read on a Dynatech MR5000 microplate reader (Dyna- tech, Chantilly, VA, USA) at 54...

Ngày tải lên: 19/02/2014, 06:20

... for experimental work, BAPTA and its analogues may also find important therapeutic applications in diseases [1 0–1 3]. In particularly, BAPTA can attenuate neurotransmitter release in central mammalian synapses ... doi:10.1111/j.1432-1033.2004.04259.x D-BAPTA AM, a BAPTA derivative lacking one acetic acid group was prepared by chemical synthesis (CEA Saclay, France). BAPTA and BAPTA...

Ngày tải lên: 19/02/2014, 16:20

... from mitochondria, and apoptotic cell death. Radiation also induced transcrip- tional upregulation of Fas, caspase-8 activation, Bax and Bak activation, and phosphorylation and downregulation ... to amplify mitochondrial apoptotic cell death in human cervical cancer cells. We show that mitochondrial cell death in response to radiation is induced by activation...

Ngày tải lên: 07/03/2014, 05:20

... receptor- associated death domain and of the TNF-related apoptosis-inducing ligand, leading to the activation of death- receptor signaling and caspase-mediated apopto- sis [59]. Activated Akt phosphorylates ... p27 Kip1 [57]. Akt mediation of GPCR -induced survival and anti-apoptotic pathways A key role of Akt is to facilitate cell survival and to prevent apoptotic ce...

Ngày tải lên: 16/03/2014, 05:20

... discovery that a naturally occurring mutation at the equivalent position in aA-crystallin, R116C, caused congenital cataract in humans [12]. In aA-crystallin and aB-crystallin, R116 and R120, respectively, ... the cell by linking adjacent myofibrils to each other, to the cell membrane, and to the nuclear envelope [13]. R116C aA-crystallin causes cataract in the lens (where...

Ngày tải lên: 16/03/2014, 18:20

... NPs are dealt in a satisfactory way both from a syntactical and from a semantical point of view. Other complex linguistic phenomena (as anaphora, quantification and ellipsis) requires a more ... of a mapping rule. A mapping rule is a list of plausible gram- matical relations that can correspond to the seman- tic relation. In a mapping rule each grammatical relatio...

Ngày tải lên: 18/03/2014, 02:20