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Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc

Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc

Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc

... (CCAAACCACGTTTACTCACCTCAGCAGCCAATACCG) for KR mutation; RKDmsAF (GCTGCTGAGGTGAGTCGCAAAGGTTTGGTAAAAACG) and RKD-msAR (CGTTTTTACCAAACCTTTGCGACTCACCTCAGCAGC) for RK mutation; and KRtoKKDmsAF(GCTGAGGTGAGTAAAAAGGGTTTGGTAAAAACGACAGCG) ... KRtoKKDmsAF(GCTGAGGTGAGTAAAAAGGGTTTGGTAAAAACGACAGCG) and KRtoKKDmsAR (CGCTGTCGTTTTTACCAAACCCTTTTTACTCACCTCAGC) for KKmutation.SDS/PAGE and western blottingProteins were separated using SDS ⁄ PAGE and ... (GGCCGAATTCACCATTATGAGCACTTTTA) and PCR_Ami- A_ EcoRI_rev (GGCCGAATTCGCTGTGTCCGTTGCTGGTT) for AmiA, and PCR_MdoD_EcoRI_for (GGCCGAATTCACCATTATGGATCGTAGAC) and PCR_Mdo-D_EcoRI rev (GGCCCAATTCGTCAAAACGCTGGGTTTGC)...
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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... artifi-cial substrate to assess the kinase activity and GST alone wasused as a negative control. The top panel shows the kinase assayvisualized by autoradiography and the bottom panel shows the ... Yonezawa M, Maruyama K, Yamaguchi-Shino-zaki K & Shinozaki K (2007) The mitogen-activatedprotein kinase cascade MKK3-MPK6 is an importantpart of the jasmonate signal transduction pathway ... kinase buffer and [c-32P]-ATP. For (A) and (B) the top panel shows the kinaseassay visualized by autoradiography and the bottom panel shows the Coomassie Brilliant Blue-stained SDS ⁄ PAGE. The...
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Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... Forward (nt 1552–1585 PAI-2)SJS260 AACTCACCATAGGAATGCATAATAAATAACAAAG Reverse (nt 1585–1552 PAI-2)SJS261 CTTTGTTAAAGCTTATGCATTCCTATGGTGAGTT Forward (nt 1552–1585 PAI-2)SJS262 AACTCACCATAGGAATGCATAAGCTTTAACAAAG ... 1491–1508 PAI-2)SJS138 TACGAGATCTTAGCTACATTAAATAGGC Reverse (nt 1620–1603 PAI-2)SJS172 GGGATCATGCCCAAGCTTATTTTCCTTACT Forward (nt 1491–1520 PAI-2)SJS173 AGTAAGGAAAATAAGCTTGGGCATGATCCC Reverse ... GACCCCTTCATTGACCTCAACTA Forward (nt 163–185 GAPDH)SJS209 CTTGATTTTGGAGGGATCTC Reverse (nt 318–299 GAPDH)SJS275 TTAGCTACATTAAATAGGCAG Reverse (nt 1620–1601 PAI-2)SJS276 GtaatacgactcactataGGGATCATGCCCATTTAG...
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Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc

Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc

... move towards a b-strand conformation at20–30 °C, and finally towards random coil at 60 °C.Residues 17–24 display a more complex temperaturebehaviour and do not all behave similarly. Two of them ... state interpretation of the CDdata. These values of Tmappear to fall near the minima of the / angle curves.For comparison we also studied the variant peptideAb(12–28)G19G20. At low temperature, ... structural groups. A true random coil has a smaller hydrodynamic radius, RH, than a PII-helix and PII has a smaller RHthan an extended b-strand.Figure 4 shows the calculated hydrodynamic radiusdependence...
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Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Purification and properties docx

Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Purification and properties docx

... Fdh1B(both at 58%) are translated from the chromosomes of M. capsulatus and of the unknown bacterial contaminant of L. major DNA (see above). In these two latter cases, the polypeptides also reveal the ... similar to the one in Eubacteriumacidaminophilum containing tupA are also found in Vibriocholerae, Campylobacter jejuni, Haloferax volcanii and Methanothermobacter thermautotrophicus, and a ... sufficient to allow quantification or the identification of the exact nature of the molybdopterin cofactor. However, the primary sequence analysis of FDH1 clearly indicatesthat all of the amino acid residues...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Discovering Global Patterns in Linguistic Networks through Spectral Analysis: A Case Study of the Consonant Inventories" pdf

... clear from these rulesthat the split into C− and C+has taken placemainly based on whether the consonants have the combined “dental alveolar” feature (negativeclass) or the “dental” and the ... is an equal prevalence of the consonants from C+ and C−in the languages of L−. Therefore, it can be argued that the pres-ence of the consonants from C−in a language can(phonologically) ... because, such a distinctionis prevalent in many other sounds, some of whichare (a) nasals in Tamil (Shanmugam, 1972) and Malayalam (Shanmugam, 1972; Ladefoged and Maddieson, 1996), (b) laterals...
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Báo cáo khoa học: The organotellurium compound ammonium trichloro(dioxoethylene-o,o¢)tellurate reacts with homocysteine to form homocystine and decreases homocysteine levels in hyperhomocysteinemic mice pptx

Báo cáo khoa học: The organotellurium compound ammonium trichloro(dioxoethylene-o,o¢)tellurate reacts with homocysteine to form homocystine and decreases homocysteine levels in hyperhomocysteinemic mice pptx

... a cleavage substrate of caspase-3that is inactive once cleaved, was used as another indi-rect marker for caspase-3-mediated apoptosis. CleavedPARP1 and the active cleaved form of caspase-3 ... penicillin and streptomycin were pur-chased from Gibco Laboratories (Grand Island, NY, USA).Caspase-3 and PARP1 antibodies were purchased from CellSignaling (Danvers, MA, USA). Antibody against a- tubulinwas ... ultrasense and a 15 mW argon ion laser with an excitation wavelength of 488 nm. The internal standard for calibration was a stock of fixed ram sperm nuclei prepared as described ear-lier. For each...
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Báo cáo khoa học: The mouse Muc5b mucin gene is transcriptionally regulated by thyroid transcription factor-1 (TTF-1) and GATA-6 transcription factors pdf

Báo cáo khoa học: The mouse Muc5b mucin gene is transcriptionally regulated by thyroid transcription factor-1 (TTF-1) and GATA-6 transcription factors pdf

... TCACAGCCTTGTTGATACTTTGGGGACT239 Mutated T242 TCACAGCCTTGTTCTTTACTTTGGGGACT254 GATA ()454 ⁄ )449) GCCCATGACCATCTGGAGCATAATConsensus GATA GATA CACTTGATAACAGAAAGTGATAACTCTRegulation ... and 19) IgGs. The DNA–protein complex (GATA) and supershifts(ss GATA-4, ss GATA-6) are indicated by an arrow on both sides of the autoradiograms. (E) In vivo binding of GATA-4, GATA-5 and GATA-6to ... mucin activation by GATA fac-tors and the terminal differentiation of the specializedepithelial cell in which mucin expression is activated.In this work, it appears that GATA-5 and GATA-6are also...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx

Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx

... 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢Vps4 Ter2 F 5¢-TTAAAAGAACCAGATTAGTCAATTGATTAACGTGCT-3¢Vps4 Ter2 R 5¢-AGCACGTTAATCAATTGACTAATCTGGTTCTTTTAA-3¢Vps4 RDE F 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢Vps4 ... 5¢-GAGAATCAGTGTCGACTTCATCTATAAAAATAATAGAAGGTTTATT-3¢Vps4 SalIF 5¢-GCCCATATTCGTCGACGCGCTAACAGGTACCAGAGGAGAAGGAGAGAGCGAAGCAAGTAG-3¢Vps4 Dstr R 5¢-GGGCGGATCCTCTGCTTTTCTTTATC-3¢Vps4 Ter1 F 5¢-GCGCTAATGCAACCGTAGTCAATTGATTAACGTGCT-3¢Vps4 ... 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢Vps4 RDE R 5¢-ATCAATTGACTAGTTACCTGCTTGACCAAAAGCTGCAGTGAACTGTTCTTGCTT-3¢Vps4 RKI F 5¢-GCGCTAATGCAACCGATAGATGTCTCTACGGAGGAC-3¢Vps4 RKI R 5¢-GTCCTCCGTAGAGACATCTATCGGTTGCATTAGCGC-3¢Vps4...
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Báo cáo khoa học: Post-ischemic brain damage: NF-jB dimer heterogeneity as a molecular determinant of neuron vulnerability pdf

Báo cáo khoa học: Post-ischemic brain damage: NF-jB dimer heterogeneity as a molecular determinant of neuron vulnerability pdf

... namely the ‘classic’ pathway and the ‘alternative’ pathway, which result in the release of NF-jB from its inhibitors and in the nuclear localiza-tion of NF-jB [7]. The canonical pathway of NF-jBactivation ... signal transducer and activator of tran-scription 3 and nuclear factor-kappaB (NF-j B) [1].NF-jB is a key regulator of both inflammation and cell death and has been proposed as suitable target ... generation of reactive oxygenspecies, DNA damage and inflammation. In focalischemia, primary neuronal death appears rapidly in the core area and is followed by secondary death in the ischemic penumbra...
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