Báo cáo khoa học: Electron-transfer subunits of the NiFe hydrogenases in Thiocapsa roseopersicina BBS pptx

... reduced the in vivo activities of the hydrogenases, although they were fully active in vitro. The absence of HupC resulted in a decrease in HupSL activity in the membrane, but removal of the Isp1 ... the same time, the in vitro activity was twice as high as that of the strain harbouring HupC (Table 5). A comparison of the hupSL mRNA levels of the...

Ngày tải lên: 16/03/2014, 03:20

... [115] reported the finding of two potential binding sites for HIF-1 in the first intron of the PK-M gene. On the other hand, Yamada and Noguchi [116] reported that there is no HIF-1 binding sequence ... [70]. In melanoma cells, eleva- tion of the cellular Ca 2+ concentration leads to detachment of PFK from the cytoskeleton and thus diminishes the provision of local A...

Ngày tải lên: 14/03/2014, 23:20

... preventing binding of oxygen. We analyzed the azide inhibition and determined the inhibition constants for sodium azide, in order to see the effect of the mutation (L559A) on the binding properties ... stability of the trinuclear site is decreased, the binding of azide to the trinuclear site may be decreased. The depletion of the T2 copper and chloride ion may...

Ngày tải lên: 16/03/2014, 00:20

... [5]. Furthermore, a chromosomal point mutatio n in srfA-B, changing a D to A in the His-motif (italic) of the corresponding Asp domain of the surfactin synthetase was found to abolish surfactin production ... bound in the binding poc ket o f a nother e nzyme molecule within the active homomultimer. This could explain the distance in sequence homology with NRPSs C-domai...

Ngày tải lên: 17/03/2014, 11:20

... chymotrypsin numbering) is involved in the formation of a salt bridge with the N-terminal Ile of the mature enzyme for completing the formation of Fig. 2. Distribution of charged amino acids in Panulirus ... at the junction of the two barrels. Another conserved characteristic of lobster trypsins is the presence of calcium-binding sites (Fig. 3C). The calcium-...

Ngày tải lên: 23/03/2014, 03:20

... extreme modulations of enzyme activities. In particular, in the combinations involving the decrease of glyoxalase II activity the effect is equival- ent to the modulation of the other parameters ... HPLC analysis of the glyoxalase pathway metabolites in Leishmania infantum promastigotes. (A) Analysis of 2-oxoalde- hydes, showing the presence of MG as 2-methylqu...

Ngày tải lên: 23/03/2014, 13:20

... cleavage of the linker [22]. Although these results provided valuable suggestions for the role of the P-gp linker region, the molecular details, such as the involvement of the linker region in substrate recognition, ... exclude the effects of interacting proteins but can also be used to perform kinetic analy- ses of the native and linker-cleaved P-gp. In the pr...

Ngày tải lên: 29/03/2014, 23:20

... behalf In this case, the binding should take place in two steps, the flagging of the need for a forward binding and the moving of the pointer from the antecedent, once detected, to the flag. ... concerns fragments of it. At any point of the process this structure contains the current hypothesis about the analysis of the parsed segment of the input...

Ngày tải lên: 24/03/2014, 05:21

... their amino acids, with the a subunits being the most conserved and the b subunits the least conserved proteins (Table 2). The similarity scores of nonorthologous subunits is in the range of 55–64%. ... with the e-type subunits. A hemocyanin-based timescale for the evolution of the Chelicerata The orthologous hemocyanin subunits of E. californicum and N...

Ngày tải lên: 08/03/2014, 08:20

... excluded all residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range 10–200. ApeSOD ... focus on the coordination of the metal cofactor in the active site as well as the changes it experiences in response to different metal cofactors. Finally, by c...

Ngày tải lên: 14/02/2014, 22:20