Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx

... functional domains, to deter- mine the pattern of covalent attachment of substrates and intermediates to active sites during catalysis. Using the natural substrates, the acyltransferase and acylcarrier ... (AT1) appears as two fragments corresponding to alternative sites of proteolysis; one fragment has a molecular mass of 32582 Da and the second has a molecular...

Ngày tải lên: 16/03/2014, 03:20

... that the EcR ⁄ Locusta migratoria RXR (EcR:LmRXR) heterodi- mers are activated at lower concentrations of ligand when compared with the concentrations of ligand required for the activation of ... response genes and regulate their transcription. Most of the nuclear hormone receptors, including EcR, are modular and function as ligand-controlled transcription factors, a chara...

Ngày tải lên: 07/03/2014, 12:20

... drafting the manuscript. MG participated in analysis and interpretation of the data and in drafting the manuscript. PS, JS and MV contributed to the conception and design of the study and manuscript ... revision. JK was involved in the design and statistical analysis of the study. MS conceived and coordinated the study and was involved in the interpretation of the...

Ngày tải lên: 25/10/2012, 10:39

... usually lack a TATA-box and employ multiple transcriptional start sites [10,14]. Mutation of either a CDE or a CHR in a promoter leads to the activation of tran- scription in quiescent cells. A ... regulation. The CDE alone was not tested [41]. The CDE mutation that was assayed would also alter a putative CDE site with the standard dis- tance of four nucleotides to...

Ngày tải lên: 16/02/2014, 09:20

... oligomeri- zation affinity of CA and the stability balance of the HIV capsid may be a result, in part, of the preserva- tion of intersubunit electrostatic repulsions. Conformational rearrangements of CA and ... equilib- rium constant of the association step for each single mutant. It was found that removal of the side chain interactions of any one of almost half...

Ngày tải lên: 18/02/2014, 06:20

... the assembly-competent mutants E18 7A and N18 3A: the conformations of the two assembly-incompetent mutants (Y16 9A and L21 1A) in the absence of CAI are the same as the structures of any mutant when complexed to the ... CA assembly. Variants of CAI peptide Armed with this structural information on CAI, Zhang et al. [32] used a structure-based rational design appro...

Ngày tải lên: 18/02/2014, 06:20

... pro-peptides appear to be diver- gent within the TGFb ⁄ BMP family, and appear to modulate the function of the mature part by non- covalent association after proteolytic cleavage by pro-hormone convertases ... induce p38 and Smad phosphorylation. The data presented here suggest that the pro-domain of BMP-2 can alter the signalling properties of the growth factor by...

Ngày tải lên: 18/02/2014, 06:20

... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization of ACMSD from Pseudomonas fluorescens reveals a penta- coordinate mononuclear metallocofactor. J Am ... nonoxidative decarboxylation catalysed by a- amino-b-carboxymuconate-e-semialdehyde decarbox- ylase. J Am Chem Soc 129, 9278–9279. 20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashi...

Ngày tải lên: 18/02/2014, 06:20

... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC AATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAG AGCCTTACAAGAAGGGTCCAAAGA-3¢). The PCR product was purified, treated with T4 exonuclease to create vector-compatible overhangs and annealed ... degradation of (GlcNAc) 3 (r) and (GlcNAc) 4 ( )byLlChi1 8A, illustrated by the production of (GlcNAc) 2 during the initial linear phase of the degradation r...

Ngày tải lên: 18/02/2014, 08:20

... mem- branes, was analyzed by CD. As already seen with TFE, at increasing concentrations of SDS J1_tmic undergoes a significant conformational change towards an a- helical structure, reaching a maximum of ... dependent on pH, sug- gesting that the conformational change is driven by polar, rather than hydrophobic interactions. The partial folding of the cytoplasmic domain...

Ngày tải lên: 18/02/2014, 16:20