Báo cáo khoa học: Regulation of calpain B from Drosophila melanogaster by phosphorylation pot
Báo cáo khoa học: Regulation of calpain B from Drosophila melanogaster by phosphorylation pot
... 193,
886–887.
Phosphorylation of < /b> calpain < /b> B L. Kova
´
cs et al.
4972 FEBS Journal 276 (2009) 4959–4972 ª 2009 The Authors Journal compilation ª 2009 FEBS
Regulation < /b> of < /b> calpain < /b> B from Drosophila melanogaster by
phosphorylation
La
´
szlo
´
Kova
´
cs
1,
*, ... was determined by the
Bradford assay [16].
Protein phosphorylation
In vitro phosp...
Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
... synthesis described above are very similar from
a regulatory point of < /b> view. dCMP deaminase is activa-
ted by dCTP and inhibited by dTTP and both nucleo-
tides act on the enzyme by binding to an ... also
investigated by equilibrium binding. This revealed a
hyperbolic binding curve (Fig. 4D) with a stochiometry
of < /b> 1 : 1 of < /b> dTTP bound per subunit of < /b> dCTP
de...
Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx
... interaction between 2A2 mAb and
cathepsin B.
Determination of < /b> the 2A2 mAb binding site on
cathepsin B
The binding site of < /b> 2A2 mAb on cathepsin B was
determined by SPOT analysis (SPOTs System; ... by a two-step mechanism in which
the N-terminus of < /b> the inhibitor first binds to the
enzyme, displacing the occluding loop, followed by the
binding of < /b> another tw...
Báo cáo khoa học: Modulation of amyloid-b aggregation and toxicity by inosose stereoisomers pot
... the pool of < /b> soluble, potentially
toxic Ab species. To investigate the disassembly of
< /b> fibers, three separate preparations of < /b> preformed Ab40
and Ab42 fibers were incubated in the presence of
< /b> varying ... inhibition of < /b> Ab olig-
omerization and fiber formation. This, however, does
not preclude any interaction with Ab. scyllo-Inosose is
able to bind to Ab, as illustr...
Báo cáo khoa học: Regulation of luteinizing hormone receptor mRNA expression by mevalonate kinase – role of the catalytic center in mRNA recognition potx
... repre-
sentative of < /b> three independent experiments. (B) The protein bands
were quantified by densitometric scanning followed by analysis
using
NIH IMAGE 1.61 software (mean value ± SE).
Active site of < /b> LRBP involved ... for binding,
RNA mobility shift analysis was performed using
[
32
P]-labeled LBS with all the double mutants in the
absence and presence of < /b> a 10-fold mol...
Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
... length of < /b> IFN -b mRNA was analysed by Northern blot, using a
32
P-labelled antisense IFN -b riboprobe. As a control, the
membrane was hybridized with a GAPDH antisense riboprobe. (C) Total RNA of < /b> cells ... generated by in vitro transcription with T3 or
Sp6 polymerases. RNA was quantified by absorbance at
260 nm and its integrity was verified by agarose gel
electrophoresi...
Báo cáo khoa học: Characterization of thermostable aminoacylase from hyperthermophilic archaeon Pyrococcus horikoshii pot
... (PDB ID: 1XMB). The nickel ions binding to BsbAH are shown by a pink sphere.
(B) Speculated zinc-binding domain of < /b> PhoACY. Zinc ion is shown by a pink sphere. The residues related to zinc binding ... suggesting
AB
Fig. 5. Molecular model presentation of < /b> PhoACY and BsbAH. (A) Superimposition of < /b> PhoACY (blue) on BsbAH (PDB ID: 1YSJ) (yellow). The
model of < /b> PhoACY...
Báo cáo khoa học: Implication of calpain in neuronal apoptosis A possible regulation of Alzheimer’s disease doc
... cleavage of < /b> Bid by calpain < /b> and
formation of < /b> truncated Bid (tBid) leading to
(2a) direct release of < /b> cytochrome c and ⁄ or
( 2b) permeabilization of < /b> mitochondrial outer
membrane, translocation of < /b> calpain < /b> ... illustrating the regulation < /b> of
< /b> Bcl-2 family proteins, cytochrome c release
and apoptosis by calpains. (1) Cleavage of
< /...
Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... dissociation of < /b> Bax and Bad from 14-3-3 is pro-
moted by JNK via phosphorylation of < /b> 14-3-3 at
Ser184; this reduces the affinity of < /b> 14-3-3 for Bax and
Bad and translocation of < /b> Bax and Bad to mitochon-
dria ... Bcl-xL, Bcl-w, Mcl-1 and A1), pro-apoptotic
proteins (Bax, Bak and Bok) and BH3-only proteins
(Bad, Bid, Bik, Blk, Hrk, BNIP3 and BimL). Bcl-2
family pro...
Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc
... DNA-binding activity is
increased, but the amount of < /b> HSF2 protein simultaneously decreases, at least in part, because of < /b> enhanced ubiquitination by the E3 ligase
APC ⁄ C followed by degradation by ... capacity of < /b> HSF1 is
repressed through a negative-feedback loop via binding of < /b> HSPs.
The DNA-binding activity of < /b> HSF1 is inhibited by acetylation of <...