Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electron paramagnetic resonance pptx

... substrate protein [21–23]. The 19S caps are involved in recognition of the polyubiquitinated protein substrates, unfolding of the condemned protein [24], removing ubiquitin chains for recycling [25,26] ... Authors Journal compilation ª 2009 FEBS MINIREVIEW New roles of flavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of...

Ngày tải lên: 16/03/2014, 02:20

... Journal compilation ª 2009 FEBS 4303 MINIREVIEW New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electron paramagnetic resonance Erik Schleicher 1 , Robert ... are interacting only very weakly with the unpaired electron spin, e.g. protons from the protein backbone within the cofactor binding pocket, protons o...

Ngày tải lên: 16/03/2014, 02:20

... MINIREVIEW New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin Federico Forneris 1 , Elena Battaglioli 2 , Andrea Mattevi 1 and Claudia Binda 1 1 ... is in agreement with the domain organiza- tion in the first line. The third line shows a summary of the identified linear motifs (after filtering processing taking in account the acc...

Ngày tải lên: 16/03/2014, 02:20

... of the Fe(II) spin state and no replacement of any of the two axial ligands of the iron, His18 or RNO, by an amino acid side-chain of the antibody protein. Binding site topology of antibody 3A3 Figure ... the binding of MP8 occurred through interactions of its carboxylate substituents with amino acids of the antibody, and that the protein provided a partial steric hindranc...

Ngày tải lên: 19/02/2014, 12:20

... potential of the haem, rate constants o f CO, O 2 and CN b inding to haem, and CD spectra in the Soret region [15–18], suggesting that this residue is critical for maintaining the electronic states of ... ncubated for 10 min prior to spectroscopic measurements. Cyanide binding The association rate of cyanide to haem was observed by monitoring changes at 417 nm in spectra of the...

Ngày tải lên: 19/02/2014, 16:20

... counterparts, in agreement with the higher mass of BChE subunits [37]. This indicates a predominant in uence of the catalytic domain on oligomerization. Role of the C-terminal cysteine Mutation of the ... presence of this cys- teine retains the enzyme intracellularly. In case of Aa, the cellular activity of AChE was also decreased by approximately 50% but secretion was no...

Ngày tải lên: 07/03/2014, 03:20

... also be involved. In multicellular organisms, GATA-binding factors play critical roles in development, including cell- fate specification, regulation of differentiation, and control of cell proliferation ... whereas the level of the c-T1 transcript remained unchanged. Ninety-eight percent of the cells were undergoing mitosis ⁄ cytokinesis at this time (as determined by count...

Ngày tải lên: 07/03/2014, 04:20

... oxida- tion of firefly luciferin with molecular oxygen in the presence of ATP and Mg 2+ , resulting in luminescence [1–3]. The stereoselectivity of this bioluminescent reac- tion was investigated in detail ... of ketoprofen and flurbiprofen were 9.5 min and 13.2 min, respectively. Each assay was performed eight times. The kinetic parameters for racemic ketoprofen were determined...

Ngày tải lên: 07/03/2014, 10:20

... and C-terminal domain, including the highly conserved GSH-binding site (G-site) and the more divergent cosubstrate-binding domain or hydrophobic binding site (H-site) [7,18]. The G-site includes ... arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH-binding or cysteine-binding proteins. The H-site is entirely helical, with a variable number of...

Ngày tải lên: 15/03/2014, 09:20

... phase of the L99F mutant was independent of the O 2 concentration. b Measurement of the rate of O 2 binding was not feasible because of low heme binding affinity and instability of the protein. A C B D Fig. ... stable Fe(II)–O 2 complex. CO binding kinetics We next examined the kinetics of CO binding for the mutant proteins of Ec DosH using laser flash photo- lysis (Fig. 6 and T...

Ngày tải lên: 16/03/2014, 13:20