Báo cáo khoa học: Weak oligomerization of low-molecular-weight protein tyrosine phosphatase is conserved from mammals to bacteria pot

... for useful discussions. This work was partially supported by funds from the Spanish Ministry of Education (BIO2007-63458 to MP). J.B. is a recipient of a pre- doctoral fellowship from the Spanish Ministerio ... Authors Journal compilation ª 2009 FEBS 4357 Weak oligomerization of low-molecular-weight protein tyrosine phosphatase is conserved from mammals to...

Ngày tải lên: 16/03/2014, 02:20

... D 2 dopamine receptor is a member of the D 2 -like family of dopamine receptors (which comprises D 2 ,D 3 and D 4 receptors). These receptors are GPCRs that couple to G proteins of the G i/o family. ... receptors. One of the major characteristics of the baculovirus expression system is that the insect cells tend to overexpress exogenous proteins [36–38]. Overexpression of...

Ngày tải lên: 21/02/2014, 00:20

... molecular mechanism by which volvatoxin A1 inhibits the cytotoxicity of volvatoxin A2. Volvatoxin A1 by itself was found to be nontoxic, and furthermore, it inhibited the hemolytic and cytotoxic activit- ies ... molecules of volvatoxin A2, each of which contains one amphi- pathic a-helix. Taken together, the results of this study reveal a novel mechanism by which volvatoxin A1 regul...

Ngày tải lên: 19/02/2014, 06:20

... of Biochemistry, Lund University, Sweden During biosynthesis of chlorophyll, Mg 2+ is inserted into protoporphyrin IX by magnesium chelatase. This enzyme consists of three different subunits of ... it is well-known that Mg 2+ is required to perform the ATP hydrolysis. In addition, kinetic analysis had shown binding of Mg 2+ to this subunit [21]. An integrin I domain, sugges...

Ngày tải lên: 19/02/2014, 12:20

... indicate the absence of stereochemical distortions. An assessment of the local variability of backbone and heavy atoms positions is provided by the histogram of the rmsd (Fig. 4, bottom). As expected, ... from C. durissus terrificus [2]; MYX_CROVV, myotoxin a from C. viridis viridis [15]; MYXC_CROVH, peptide C from C. v. helleri [16]; MYX1_CROVC and MYX2_ CROVC, myotoxin I and...

Ngày tải lên: 20/02/2014, 11:20

... to be defined. We hypothesize that a variety of tissues may express basal levels of a TF/factor VII- independent procoagulant. Indeed, constitutive expression of TF in some cells and tissues is ... glomerulonephritis [4,5], septic shock [6,7], and bacterial and viral infections [8]. For example, tissue factor (TF) [9], the transmembrane receptor of factor VII, is the major procoa...

Ngày tải lên: 20/02/2014, 11:20

... Quantitation and comparison of proteins in GM and WI-38 cells. From the data in Fig. 2 and others, the concentrations of Mcm proteins, ORC2 and PCNA in total protein and chromatin-bound protein were compared ... albeit the total proteins and histone were present at comparable levels. The higher level of Mcm expression was detected not only in total cellular proteins but also in ch...

Ngày tải lên: 20/02/2014, 23:20

... induces rotation of Tyr370 ⁄ His375, movement of the loop region (N167–V177), and flip-flop of Tyr170, resulting in the transition from open to closed forms. From the comparison of the three-dimensional ... b-lactam hydrolysis [16–20]. In Hyb-24DNY, Asn266-O d is 5.0 A ˚ away from Ser112- O c , and this distance is slightly larger than the distance between Ser70-O c and Glu1...

Ngày tải lên: 07/03/2014, 00:20

... response of pyrene–tropomyosin fluorescence to S1 binding led to the idea that this probe measures entry into the most active state of actin, but is insensitive to transitions to states of intermediate ... activity of an individual state. Only state A O supports myosin activ- ity, so it is necessary to go stepwise from the blocked to the closed to the open states. T...

Ngày tải lên: 07/03/2014, 09:20

... assumption of weak enzyme bind- ing. r is a measure of the stimulus strength. The analysis of nonsequential phosphorylation mech- anisms is generally more complicated, due to the large number of phosphorylation ... mechanism. In these cases, a much higher ratio of kinase activity to phosphatase concentration is needed to achieve complete phosphory- lation of the ta...

Ngày tải lên: 07/03/2014, 10:20