Báo cáo khoa học: Weak oligomerization of low-molecular-weight protein tyrosine phosphatase is conserved from mammals to bacteria pot

... foruseful discussions. This work was partially supportedby funds from the Spanish Ministry of Education(BIO2007-63458 to MP). J.B. is a recipient of a pre-doctoral fellowship from the Spanish Ministerio ... Authors Journal compilation ª 2009 FEBS 4357 Weak oligomerization of low-molecular-weight protein tyrosine phosphatase is conserved from mammals to bacteria Jascha Blobel1, Pau Bernado´1, ... interaction of bacteria witheukaryotic hosts. As such, the comparison with BPTP is expected to genuinely display the effects of evolution inlmwPTPs of two phylogenetically distant organisms.The...

Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved ﬂuorescence resonance energy transfer pdf

... D2dopamine receptor is a member of the D2-likefamily of dopamine receptors (which comprises D2,D3and D4receptors). These receptors are GPCRs thatcouple to G proteins of the Gi/ofamily. ... receptors. One of themajor characteristics of the baculovirus expression system is that the insect cells tend to overexpress exogenous proteins[36–38]. Overexpression of receptors can be a factor ... receptorsdescribed suggests that the phenomenon of oligomerization may be general to the whole GPCR family, rather thanbeing restricted to some subgroups of receptors. Hence, oligomerization of...

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

... molecular mechanism by which volvatoxin A1 inhibits thecytotoxicity of volvatoxin A2. Volvatoxin A1 by itself was found to benontoxic, and furthermore, it inhibited the hemolytic and cytotoxic activit-ies ... molecules of volvatoxin A2, each of which contains one amphi-pathic a-helix. Taken together, the results of this study reveal a novelmechanism by which volvatoxin A1 regulates the cytotoxicity of ... Aneffect of the cardiotoxic protein volvatoxin A on thefunction and structure of heart muscle cells. J Mol CellCardiol 8, 501–519.3 Lin JY & Shi GY (1976) Toxicity of Volvatoxin Aisolated from...

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... of Biochemistry, Lund University,SwedenDuring biosynthesis of chlorophyll, Mg2+ is inserted intoprotoporphyrin IX by magnesium chelatase. This enzymeconsists of three diﬀerent subunits of ... it is well-known that Mg2+ is required to performthe ATP hydrolysis. In addition, kinetic analysis had shownbinding of Mg2+ to this subunit [21]. An integrin I domain,suggested to exist ... AAA+proteins represent one type of molecular chaperones and their function is to control thefate of proteins or DNA. This is done by facilitating protein folding and unfolding, assembly and disassembly...

Tài liệu Báo cáo khoa học: Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terriﬁcus venom docx

... indicate the absence of stereochemical distortions.An assessment of the local variability of backbone andheavy atoms positions is provided by the histogram of thermsd (Fig. 4, bottom). As expected, ... from C. durissus terriﬁcus [2];MYX_CROVV, myotoxin a from C. viridis viridis [15]; MYXC_CROVH, peptide C from C. v. helleri [16]; MYX1_CROVC and MYX2_CROVC, myotoxin I and II from C. viridis ... 2). This last spectralfeature, generally unusual for globular proteins, is sharedby a number of other proteins, including toxins such ascobratoxin, erabutoxin b, myotoxins and a-neurotoxins[34,35].The...

Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

... to be deﬁned. We hypothesize that avariety of tissues may express basal levels of a TF/factor VII-independent procoagulant. Indeed, constitutive expression of TF in some cells and tissues is ... glomerulonephritis [4,5], septic shock [6,7],and bacterial and viral infections [8]. For example, tissuefactor (TF) [9], the transmembrane receptor of factor VII, is the major procoagulant of the classical ... weeks of age were purchased from Jackson Laboratories (Bar Harbor, ME), and werehoused in the animal facility at the Toronto HospitalResearch Institute, University of Toronto.CellsPeritoneal...

Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... Quantitation and comparison of proteins in GM and WI-38cells. From the data in Fig. 2 and others, the concentrations of Mcmproteins, ORC2 and PCNA in total protein and chromatin-bound protein were compared ... albeitthe total proteins and histone were present at comparablelevels. The higher level of Mcm expression was detected notonly in total cellular proteins but also in chromatin-boundproteins. ... Mcm proteins bound to chromatin probablyfunction in DNA replication, but the role of the proteins nottightly bound to chromatin remains to be determined.Consistent with the results at the protein...

Báo cáo khoa học: Molecular design of a nylon-6 byproduct-degrading enzyme from a carboxylesterase with a b-lactamase fold ppt

... inducesrotation of Tyr370 ⁄ His375, movement of the loop region (N167–V177),and ﬂip-ﬂop of Tyr170, resulting in the transition from open to closedforms. From the comparison of the three-dimensional ... b-lactam hydrolysis [16–20]. InHyb-24DNY, Asn266-Od is 5.0 A˚away from Ser112-Oc, and this distance is slightly larger than the distancebetween Ser70-Ocand Glu166-Oe2 of b-lactamase.However, ... [13]. The amount of the Hyb-24 mutant protein included in the cell extract was quantiﬁed by densito-metric analysis of protein bands separated by SDS ⁄PAGE using nih image analysis software (http://rsb.info.nih.gov/nih-image/)...

Báo cáo khoa học: Equilibrium distribution of skeletal actin–tropomyosin– troponin states, determined by pyrene–tropomyosin ﬂuorescence potx

... response of pyrene–tropomyosin ﬂuorescence to S1binding led to the idea that this probe measures entryinto the most active state of actin, but is insensitive to transitions to states of intermediate ... activity of anindividual state. Only state AOsupports myosin activ-ity, so it is necessary to go stepwise from the blocked to the closed to the open states. The model shown inFig. 2C is from ... & Eisenberg E (1981)Mechanism of action of troponin-tropomyosin:inhibition of actomyosin ATPase activity withoutinhibition of myosin binding to actin. J Biol Chem256, 575–578.36 Tobacman...

Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... assumption of weak enzyme bind-ing. r is a measure of the stimulus strength.The analysis of nonsequential phosphorylation mech-anisms is generally more complicated, due to the largenumber of phosphorylation ... mechanism. In these cases,a much higher ratio of kinase activity to phosphatase concentration is needed to achieve complete phosphory-lation of the target protein. Because the number of inter-mediate ... values, canequally well give rise to graded responses.We have been able to deﬁne the general condi-tions for bistability and hysteresis arising solely from multisite phosphorylation, which has...

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