Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx

... al. Human Ki-1 ⁄ 57 and pre-mRNA splicing FEBS Journal 276 (2009) 3770–3783 ª 2009 The Authors Journal compilation ª 2009 FEBS 3783 Functional association of human Ki-1 ⁄ 57 with pre-mRNA splicing ... experiments. Influence of Ki-1 ⁄ 57 on E1A pre-mRNA splicing in vivo The association of Ki-1 ⁄ 57 with splicing proteins pointed to a possible functional role...

Ngày tải lên: 16/03/2014, 02:20

... presence of transmembrane segments often interferes with the performance of integral membrane proteins in two-hybrid assays. Interestingly, Pex1p clearly interacted with the cytoso- lic part of Pex15p. ... membrane. Association of the importomer and the AAA complex in import mutants To determine whether the absence of Pex1p, Pex6p or Pex15p has an influence on the association...

Ngày tải lên: 07/03/2014, 12:20

... 1.647 nm (confidence interval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28), respectively. These ... versions of GEV format switches were made by replacing helix 12 of EcR with VP16 activation domain. This version of GEV supported constitutive expression of reporter...

Ngày tải lên: 16/03/2014, 12:20

... for the rest of the cell cycle (10 and 20 h), correlating with the expression of the gene (Fig. 6C,D). The correlation of promoter occupancy of MLL1, H3K4 trimethylation and RNAP II with Hox gene ... expression of cyclin- dependent kinase inhibitors, such as p27 and p18 [36,37]. Knockdown of HCF1 results in cell cycle arrest at G1. Therefore, both physical and functional int...

Ngày tải lên: 23/03/2014, 06:20

... transcription of mce-1, lines of transgenic nema- todes were created. The basic strategy involved the insertion of several fragments of the 5¢-region of mce-1 into the mul- tiple cloning site of the ... column. The column was washed with 10 mL of equilibration buffer, and 20 mL of equilibration buffer plus 40 mm imidazole. Then, the col- umn was eluted with 3 mL of equil...

Ngày tải lên: 23/03/2014, 13:20

... Functional analysis of disease-causing mutations in human galactokinase David J. Timson and Richard J. Reece School of Biological Sciences, University of Manchester, Manchester, ... committed step in the catabolism of galactose. The sugar is phosphor- ylated at position 1 at the expense of ATP. Lack of fully functional galactokinase is one cause of the inherited disease ga...

Ngày tải lên: 08/03/2014, 02:20

... the optimization of reaction conditions were performed in mic- rodialyzers (Spectrum Laboratories, Rancho Dominguez, CA, USA) with a molecular mass cut-off of 25 kDa in an RM volume of 70 lL with an RM ... equil- ibrated in column buffer with 0.1% Brij78. Chromatography was performed at a flow rate of 1 mLÆmin )1 with washing steps of six column volumes of column buffer sup...

Ngày tải lên: 16/03/2014, 10:20

... Hung HC (2006) Determinants of the dual cofactor specificity and substrate cooperativity of the human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of glutamine 362. J Biol Chem ... Under these conditions, 1 unit of the enzyme was defined as the amount of enzyme catalyzing the produc- tion of 1 lmol of NADH per min. An extinction coefficient of 6.22 per mm fo...

Ngày tải lên: 23/03/2014, 06:20

... purification of human elastase was based on the method of [44]. Three volumes of sucrose extraction buffer (0.1 m sodium phosphate, 0.2 m sucrose, 1 m NaCl, pH 6.0) were added to 50 mL of human blood ... sequence similarity with the N-terminal WAP domain of SLPI and only 36% sequence similarity with the C-terminal antiprotease WAP domain of SLPI [30]. This observation, com-...

Ngày tải lên: 23/03/2014, 07:20

... volume of the side-chain that are likely to disrupt the local fold. These two residues, together with N34, are located within 0.15 nm of each other and all are at, or close to, the surface of the ... Authors Journal compilation ª 2005 FEBS with the level of biochemical impairment of the puri- fied protein. The mutation, V94M, associated with the generalized form of the diseas...

Ngày tải lên: 23/03/2014, 11:20