Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
... al. Human Ki-1 ⁄ 57 and pre-mRNA splicing
FEBS Journal 276 (2009) 3770–3783 ª 2009 The Authors Journal compilation ª 2009 FEBS 3783
Functional association of human Ki-1
⁄
57 with pre-mRNA
splicing ... experiments.
Influence of Ki-1
⁄
57 on E1A pre-mRNA splicing
in vivo
The association of Ki-1 ⁄ 57 with splicing proteins
pointed to a possible functional role...
Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx
... presence of transmembrane
segments often interferes with the performance of
integral membrane proteins in two-hybrid assays.
Interestingly, Pex1p clearly interacted with the cytoso-
lic part of Pex15p. ... membrane.
Association of the importomer and the AAA
complex in import mutants
To determine whether the absence of Pex1p, Pex6p or
Pex15p has an influence on the association...
Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx
... 1.647 nm (confidence
interval of )0.5963 to 3.891, R2 of 0.9292, degree of
freedom of 10) and 1.447 nm (confidence interval of
0.9588–1.935, R2 of 0.9788, degree of freedom of 28),
respectively. These ... versions
of GEV format switches were made by replacing
helix 12 of EcR with VP16 activation domain. This
version of GEV supported constitutive expression of
reporter...
Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
... for the rest of
the cell cycle (10 and 20 h), correlating with the
expression of the gene (Fig. 6C,D). The correlation of
promoter occupancy of MLL1, H3K4 trimethylation
and RNAP II with Hox gene ... expression of cyclin-
dependent kinase inhibitors, such as p27 and p18
[36,37]. Knockdown of HCF1 results in cell cycle
arrest at G1. Therefore, both physical and functional
int...
Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx
... transcription of mce-1, lines of transgenic nema-
todes were created. The basic strategy involved the insertion
of several fragments of the 5¢-region of mce-1 into the mul-
tiple cloning site of the ... column. The column
was washed with 10 mL of equilibration buffer, and 20 mL
of equilibration buffer plus 40 mm imidazole. Then, the col-
umn was eluted with 3 mL of equil...
Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
... Functional analysis of disease-causing mutations in human
galactokinase
David J. Timson and Richard J. Reece
School of Biological Sciences, University of Manchester, Manchester, ... committed
step in the catabolism of galactose. The sugar is phosphor-
ylated at position 1 at the expense of ATP. Lack of fully
functional galactokinase is one cause of the inherited disease
ga...
Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... the
optimization of reaction conditions were performed in mic-
rodialyzers (Spectrum Laboratories, Rancho Dominguez,
CA, USA) with a molecular mass cut-off of 25 kDa in an
RM volume of 70 lL with an RM ... equil-
ibrated in column buffer with 0.1% Brij78. Chromatography
was performed at a flow rate of 1 mLÆmin
)1
with washing
steps of six column volumes of column buffer sup...
Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
... Hung HC (2006)
Determinants of the dual cofactor specificity and
substrate cooperativity of the human mitochondrial
NAD(P)+-dependent malic enzyme: functional roles of
glutamine 362. J Biol Chem ... Under these conditions, 1 unit of the enzyme
was defined as the amount of enzyme catalyzing the produc-
tion of 1 lmol of NADH per min. An extinction coefficient
of 6.22 per mm fo...
Báo cáo khoa học: Functional domains of the human epididymal protease inhibitor, eppin docx
... purification of human elastase was based on the method
of [44]. Three volumes of sucrose extraction buffer (0.1 m
sodium phosphate, 0.2 m sucrose, 1 m NaCl, pH 6.0) were
added to 50 mL of human blood ... sequence similarity with the
N-terminal WAP domain of SLPI and only 36%
sequence similarity with the C-terminal antiprotease
WAP domain of SLPI [30]. This observation, com-...
Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... volume of the
side-chain that are likely to disrupt the local fold.
These two residues, together with N34, are located
within 0.15 nm of each other and all are at, or close
to, the surface of the ... Authors Journal compilation ª 2005 FEBS
with the level of biochemical impairment of the puri-
fied protein. The mutation, V94M, associated with the
generalized form of the diseas...
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