Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... compilation ª 2009 FEBS 3769 Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 ... prevention of the effect of insulin on Foxa2 localization and lead-ing to the abrogation of insulin inhibition of gluconeo-genesis and glucose output in HepG2 cells. AlthoughTNFa-mediated inhibition ... pri-marily reflected by the attenuation of the insulin- medi-ated inhibition of gluconeogenic gene expression. AsEffect of TNFa on insulin action on PEPCK expression A. K. Pandey et al.3758 FEBS...
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Báo cáo khoa học: pyr RNA binding to the Bacillus caldolyticus PyrR attenuation protein – characterization and regulation by uridine and guanosine nucleotides potx
... nutrient-starved cells [26], and conversion of GTP to ppGpp and pppGpp during the stringentresponse to amino acid starvation [25]. A reduction in intracellular GTP without a corresponding decline in uridine ... (ATCase), the enzyme encoded by pyrB, the third cistron of the operon, provided a convenientmeasure of operon expression in such experiments.Inclusion of guanosine in the growth mediumincreased the ... leader of the operon (bindingloop 1 or BL1), between the first cistron of the operon,pyrR, and the second cistron pyrP (BL2), and betweenpyrP and the third cistron pyrB (BL3) (Fig. 1A).All of the...
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Báo cáo khoa học: "A Latent Topic Extracting Method based on Events in a Document and its Application" pot
... distribution, changing the number of topics k in the following, k = 5, k = 10, k = 20, k = 50,k = 100, and k = 200. Next, the number of topicsis fixed based on the result of the first process, and then ... number of extracted sentences is the same as used in TSC3.Precision is an evaluation measure which indicates the ratio of the number of correct sentences to that of the sentences generated by the ... evaluation measure which indicates the degree of how the system output is close to the summarygenerated by a human, taking account of the redun-dancy.Moreover, to examine the characteristics of the proposed...
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Báo cáo khoa học: Tumor necrosis factor-a converting enzyme is processed by proprotein-convertases to its mature form which is degraded upon phorbol ester stimulation pptx
... days of incubation the cells were lyzed and analyzed by Western blotting.Construction of expression vectorsBlunt end cDNAs of either bovine furin or rat PC7were cloned into pIRES1hyg (Clontech), ... degradation, we examinedwhether overexpression of functionally active furin in LoVo cells restores the effect of PMA on the degradation of mature TACE. Therefore, LoVo cells were reconstitutedwith ... primers. The obtained nucleotide sequence contained the expected nuc-leotide exchange in the furin mRNA (not shown) confirm-ing the integrity of the LoVo cell line used in ourexperiments. In conclusion,...
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Báo cáo khoa học: Tumor necrosis factor-a-induced caspase-1 gene expression pptx
... including the in ammatory response, immunity and apoptosis.Some of the responses of TNF-a are mediated by caspase-1, which isinvolved in the production of the pro -in ammatory cytokines interleukin-1b, ... lipopolysaccharide-induced septic shock and are also defective in the production of the active cytokines interleukin-1b,interleukin-18 and interleukin-33 [6–9]. Involvement of caspase-1 in TNF-a-induced ... induced by TNF-a in A549 cells and also by the overexpression of p73. Induction of cas-pase-5 by TNF-a provides further support to the sug-gestion that in human cells caspase-5 serves afunction...
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Báo cáo khoa học: Tumor necrosis factor receptor cross-talk docx
... ligand binding [31]. The intracel-lular part of TNFR1 contains a protein–protein inter- action region called the death domain [32]. Afterligand binding to the TNFR1, silencer of deathdomain ... TNFR2signaling [29]. Trimerization of TNFR2 takes placeupon binding of TNF, which leads to the recruitment of TRAF2 to the intracellular TRAF-binding motif and consequently to indirect recruitment of the TRAF2-associated ... stimulation of either TNFR1 orTNFR2 initially increased NF-jB, only TNFR2induced cell proliferation of murine fibroblasts [78].This can be explained by the signaling kinetics in the activation of...
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Báo cáo khoa học: "Impact of computerized physician order entry on medication prescription errors in the intensive care unit: a controlled cross-sectional trial"
... validated by a clinicalpharmacist. The registration of different classes of MPE wasdone according to the National Coordinating Council forMedication Error Reporting and Prevention guidelines. Anindependent ... panel, consisting of one clinical pharmacist,not involved in the registration part of the study, and two inten-sive care specialists, evaluated independently the severity of MPEs at least one month ... example of intercepted MPEinvolved the wrong prescription of a tenfold overdose of abeta-blocker, where rapid intervention of the clinical pharma-cist intercepted the administration of this...
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Báo cáo khoa học: " Effect of the medical emergency team on long-term mortality following major surgery"
... combination of a potent quinolone combined with clindamycin. This strategyis commonly followed in our facility for infection of unknownorigin and generally corresponds to the guidelines of the ... (29%), and head (18%). Two-thirds of patients had combined injuriesto the thorax (for instance, pneumo-/hemopneumothorax),including intrapulmonary contusions and lesioning, and frac-tures of the ... carbapenems, amikacin, and quinolonesonly, MRSA, sensitive to fosfomycin, rifampicin, linezolid and glycopeptides only, and S. maltophilia, sensitive to ofloxacinonly (Figure 4).Anti-infective therapyAll...
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Tài liệu Báo cáo khoa học: Mechanisms of amyloid fibril formation – focus on domain-swapping doc
... oligomerization and, by extension, amyloidformation will be considered below, including the role of 3D domain-swapping and proline isomerization. The energetics of domain-swappingIntramolecular and intermolecular ... differentconformational preferences of residues in aqueoussolution. Moreover, the additional modulation of screening by changing the local environment and inter- and ⁄ or intramolecular interactions may ... only by the proline in the cis confor-mation.Indirectly, we have confirmed that proline isomeriza-tion is at the root of the slow conformational changecoupled to tetramerization by measuring...
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Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... possible reason might be that the additional interaction between its adenine moiety and the enzymes’s adenine-binding pocket stabilizes the interaction between the enzyme a and b subunits. In contrast, ... in the activation of the enzymeÆcyanocobalamin complex. This result is consistentwith the lack of specific interaction with the adenine ring of ADP in the crystal structure of the reactivase. The ... and lower bands of the reactivase correspond to the aRÆaRbR and (aRbR)2complexes, respectively. The thin band on the front line of nondenaturing PAGE (Fig. 5A, bandiv) contained only bR(Fig....
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