Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... chain of bound hAsn in AsnO points towards the centre of the enzyme complex. The different substrate-binding mode results from conformational control of the enzyme on the side chain rotamer of the bound ... conformational control of the bound sub- strate, which enforces a gauche(–) conformer for v 1 instead of the trans conformers observed for the asparagin...

Ngày tải lên: 16/03/2014, 02:20

... In the case of con A on the other hand, binding of GlcNAc to the )1 subsite does not affect affinity [30]. This was attributed to a strained conformation of the disaccharide in the com- plex of ... also observed for GlcNAc binding in the +3 subsite [30], while for the lectin:ligand complexes of PAL this is neutral. In conclusion, the combination of stru...

Ngày tải lên: 23/03/2014, 11:20

... amine groups of K43 and K44 of monomer B, and the side chains of R421 and D12 of monomer A. These residues point towards the inte- rior of the peptide binding groove, facing opposite sides of the ... lacking the conserved glutamine that is the usual hallmark of DYNLL1 binding sequences, yet binds to DYNLL1 at the same binding site and in similar fashion. A hierar...

Ngày tải lên: 29/03/2014, 09:20

... by positioning of AZT instead of dT in its binding site (Fig. 4). In the complex of N64D and the feedback inhib- itor dTTP, the LID region closes down on the inhib- itor in the same way as in the wildtype ... were transformed into KY895 and then the strains were examined for growth, +, in the presence of different concentrations of thymidine in the me...

Ngày tải lên: 30/03/2014, 20:20

... structure of the N-domain part and the capacity of the sugar-binding site are critical in determining the specificity of the bacterial sugar-binding proteins. Evaluation of the binding affinities ... parts of the sugar-binding sites of TvuCMBP and related proteins As the sugar-binding site is formed by N- and C-domains, residues involved in sugar binding are grou...

Ngày tải lên: 19/02/2014, 00:20

... aptamers The data presented here reveal the importance of the N7 atom of purines in the organization of the Co 2 binding site in selected aptamers and their involvement in the metal ion coordination ... sites remain available for interactions with RNA ligands, including the N7 atom of purines. Therefore, there is the possible involvement of the N7 atom of...

Ngày tải lên: 16/03/2014, 06:20

... chain takes place at the second glycosidic linkage from the MeGlcA side group towards the reducing end of the xylan chain. The elucidation of the three-dimensional structure of the E. chrysanthemi ... original Slovakian government works 2107 interesting that the side chain conformations of amino acids interacting with MeGlcA 2 Xyl 3 did not change as a result of bind...

Ngày tải lên: 22/03/2014, 16:20

... sequential binding of coenzyme and substrate to the enzyme in its open con- formation followed by the closure of the interdomain cleft. In FDH, the structural ordering of the C-ter- minal helix and the ... in HGDH; Fig. 1] and is therefore referred as the nucleotide-binding domain. The strictly conserved Asp176 is the key residue in the recognition and bind...

Ngày tải lên: 23/03/2014, 13:20

... doi:10.1046/j.1432-1033.2002.02837.x In addition, the protrusion of the U towards the minor groove side of the hairpin stem may also lead to steric hindrance in the approach of UDG to DNA. On the other hand, U 15 in the U4-hairpin, ... U4-hairpin, the best substrate of the four, is located in an environment wherein both the backbone and the base conformat...

Ngày tải lên: 31/03/2014, 09:20

... advantage from the shrinkage of the interface extension. The increase in the number of strong, weak and total ion pairs at the interface of psychrophilic enzymes is important in maintaining the quaternary ... This trend increased when thermo ⁄ hyper- thermophilic enzymes were included in the working dataset. Our findings underline the role of this kind of electr...

Ngày tải lên: 30/03/2014, 03:20