... (PKA)-mediated phosphorylation of CREB at Ser133 [19]. Ser133 phos-phorylation of CREB, in turn, promotes recruitment of the coactivator paralogs CREB-binding protein and p300 via a kinase-inducible domain in CREB, ... werenormalized to b-actin or porin (for mitochondrial protein).Chromatin immunoprecipitationTreated cells were crosslinked using 1% formaldehyde for20 min and terminating with 0.1 m glycine. Cell lysateswere ... withcontinuous stirring. The temperature was then increasedslowly up to boiling point, and maintained for 10 min fordecoction. The solution was sterile filtered, concentrated and lyophilized into...