Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf
... level of Fig. 1. NGF induces expression of the microRNAs miR-221 and 222 in PC12 cells. (A) NGF- induced differentiation of PC12 cells. (B) Schematic representation of the genomic structure of miR-221 ... Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells Kazuya Terasawa 1 , Atsuhiko Ichimura 1 , Fumiaki Sato...
Ngày tải lên: 16/03/2014, 01:20
... indu ce expression of genes involved in cell cycle progression include Elk1, c-fos, c-Jun and c-myc [9,15]. The duration of ERK activation (transient or sustained) determines the repertoire of ... cell su rface. In addition it cau ses a decreased EGF bin ding affinity and tyrosine kinase activity of the r eceptor. Also shown are the stimulators and inhibitors(depictedinred)...
Ngày tải lên: 19/02/2014, 16:20
... blocked inactivation of the AMPK by insulin (Fig. 7), suggesting a dominance of the fatty acid-driven pathway for activation of AMPK over at least some aspects of insulin signalling. This dominance of ... the activation of AMPK by fatty acids and insulin and adrenergic signalling processes Fig. 5 shows studies focused on the dominant a-2 AMPK isoform. Insulin decreased...
Ngày tải lên: 07/03/2014, 15:20
Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU bind- ing homolog of enoyl-CoA hydratase’). The RNA- binding activity of the human protein ... portion of both fusion pro- teins was removed by proteolysis and the resulting pro- teins AUHp40 and AUHp30 were again purified by chromatography. Thus, four different pure fractions...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... comparative immunoblotting data, it was of interest to re-examine the fate of cals equestrin by studying the entire complement of key Ca 2+ -binding elements in dystrophin- deficient skeletal muscle ... for silver staining, hot Coomassie s taining, ÔStains-AllÕ labelling or immunoblotting. T he following running conditions were used: 60 m in at 1 00 V , 6 0 m in at 500 V, 60 min...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf
... channeling Prior investigations of channeling may be divided into two categories: in vitro and in vivo. The former include experiments with cell extracts and purified enzymes. These are inherently unsatisfying. ... studies of channeling of aminoacyl-tRNA for protein synthesis. The cells were electroporated to facilitate entry of 3 H-labeled amino- acyl-tRNA and 14 C-labeled...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... study of the role of recombination in mtDNA inheritance in S. cerevisiae showed that budding cells were enriched in linear monomers of mtDNA. It was proposed that in addition to a role in initiating ... mobility binding assays with increasing concentrations of control Ydc2 and Ydc2-ND35 (A) or Ydc2-ND35/CD15 (B), as indicated, visualized by phosphorimaging. (C) Binding...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: "Semantic Classification of Noun Phrases Using Web Counts and Learning Algorithms" ppt
... invention” and the joining phrase was by ; one of the queries would be: “invention by student” OR “invention by a student” OR “invention by the student” This returns the sum of the number of pages ... conditions, and again using all of the joining terms produced the best results. The SVM algorithm produced the best accuracy of all, achieving 50.1% accuracy using t...
Ngày tải lên: 20/02/2014, 12:20
Tài liệu Báo cáo khoa học: Regulatory modes of rod outer segment membrane guanylate cyclase differ in catalytic efficiency and Ca2+-sensitivity ppt
... Analysis of data was performed with ORIGIN 6.1 and SIGMA PLOT 4.2 software. A direct plot of activity vs. [GTP] gave in all cases a sigmoidal curve indicating cooperative substrate binding. In order ... the amount of antigen and was used to quantify the amounts of GCAPs. An example is shown in Fig. 1A,B, where the amount of GCAP standards showed a linear increase in chem...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig. 2). PCR reactions performed in ... FEBS Results Expression and purification of E. coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E. coli Pta, t...
Ngày tải lên: 06/03/2014, 11:20