Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf
Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf
... level of Fig. 1. NGF induces expression of themicroRNAs miR-221 and 222 in PC12 cells. (A) NGF- induced differentiation of PC12 cells. (B) Schematic representation of thegenomic structure of miR-221 ... Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells Kazuya Terasawa1, Atsuhiko Ichimura1, Fumiaki Sato2, Kazuharu Shimizu2 and Gozoh Tsujimoto11 ... can induce their expression. Further, our studyshowed that the sustained activation of ERK1 ⁄ 2by NGF, but not the transient activation of ERK,could effectively induce miR221 and 222 in PC12 cells. ...
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Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx
... indu ce expression of genes involved in cell cycle progression include Elk1, c-fos,c-Jun and c-myc [9,15]. The duration of ERK activation (transient or sustained) determines the repertoire of ... cell su rface. In addition it cau ses a decreased EGF bin ding affinity and tyrosinekinase activity of the r eceptor. Also shown are the stimulators and inhibitors(depictedinred)usedinthisstudy.EGFwasusedtoactivatethe ... EGF and PMA (Eur. J. Biochem. 271) 3909NRK cells with EGF, also in the presence of the PKCinhibitor bisindolylmaleimide I, and determined the ERK-PP concentration after 6 and after 60 min. In...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... blockedinactivation of the AMPK by insulin (Fig. 7), suggesting adominance of the fatty acid-driven pathway for activation of AMPK over at least some aspects of insulin signalling.This dominance of ... the activation of AMPK by fatty acids and insulin and adrenergic signalling processesFig. 5 shows studies focused on the dominant a-2 AMPKisoform. Insulin decreased a-2 AMPK activity by 55% in the ... characteristic of the fasting state. The inclusion of fatty acids resulted in increased activities of the a-1 or the a-2 isoforms of AMP-activated protein kinase (AMPK), increased phosphoryla-tion of acetyl-CoA...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... usingcrotonyl-CoA as a substrate [15]. The gene encodingthis bifunctional protein was named AUH (‘AU bind-ing homolog of enoyl-CoA hydratase’). The RNA-binding activity of the human protein ... portion of both fusion pro-teins was removed by proteolysis and the resulting pro-teins AUHp40 and AUHp30 were again purified by chromatography. Thus, four different pure fractions of AUH could ... reaction of leucine catabolism at the protein and DNA levels and developed a novel assay forenzyme analysis in a diagnostic setting. The humanAUH protein was first recognized by its ability to bindA...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... comparative immunoblotting data, it was of interestto re-examine the fate of cals equestrin by studying the entirecomplement of key Ca2+-binding elements in dystrophin-deficient skeletal muscle ... forsilver staining, hot Coomassie s taining, ÔStains-AllÕ labellingor immunoblotting. T he following running conditions wereused: 60 m in at 1 00 V , 6 0 m in at 500 V, 60 min a t 1000 V, and a final ... ffect of thedeficiency in dystrophin o n the expression of Ca2+-bindingproteins. The relatively unique combination o f the pI value and molecular mass of individual 2D spots can be useful in the...
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Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf
... channelingPrior investigations of channeling may be divided intotwo categories: in vitro and in vivo. The former includeexperiments with cell extracts and purified enzymes.These are inherently unsatisfying. ... studies of channeling of aminoacyl-tRNA for protein synthesis. The cells wereelectroporated to facilitate entry of 3H-labeled amino-acyl-tRNA and 14C-labeled free amino acids. The quan-tities of ... [14C]aminoacyl-tRNA (made in the pathwayfrom14C-labeled amino acids), [3H]aminoacyl-tRNA,14C- and 3H-labeled protein were measured. The3Hinprotein was insignificant, showing that the aminoacyl-tRNA...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... study of the role of recombination in mtDNA inheritance in S. cerevisiae showed that budding cells were enriched in linear monomers of mtDNA. It wasproposed that in addition to a role in initiating ... mobility binding assays with increasing concentrations of control Ydc2 and Ydc2-ND35 (A) or Ydc2-ND35/CD15 (B), asindicated, visualized by phosphorimaging. (C) Binding reactions withincreasing concentrations ... mitochondrial diseases and implicated in aging (reviewed in [39]). Understanding the molecularmechanisms and pathways of mtDNA inheritance in fissionyeast, alongside budding yeast and other eukaryotic...
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Tài liệu Báo cáo khoa học: "Semantic Classification of Noun Phrases Using Web Counts and Learning Algorithms" ppt
... invention” and the joining phrase was by ; one of the queries would be: “invention by student” OR “invention by a student” OR “invention by the student” This returns the sum of the number of pages ... conditions, and again using all of the joining terms produced the best results. The SVM algorithm produced the best accuracy of all, achieving 50.1% accuracy using the com-bined set of joining ... modifier-noun compounds. 2.1 Joining Terms Choosing a set of joining terms in a principled manner in the hope of capturing the semantic rela-tion between constituents in the noun phrase is dif-ficult,...
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Tài liệu Báo cáo khoa học: Regulatory modes of rod outer segment membrane guanylate cyclase differ in catalytic efficiency and Ca2+-sensitivity ppt
... Analysis of data wasperformed withORIGIN6.1 and SIGMA PLOT4.2 software. Adirect plot of activity vs. [GTP] gave in all cases a sigmoidalcurve indicating cooperative substrate binding. In order ... the amount of antigen and was used toquantify the amounts of GCAPs. An example is shown in Fig. 1A,B, where the amount of GCAP standards showed alinear increase in chemiluminescence intensity. ... data of a sigmoidal dependence in a linearLineweaver–Burk plot [41], we determined a Hill coefficientn from a fit of the direct plot. Values of Vmax and Kmwerethen determined from a plot of...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... containingthe last 326 amino acids in the case of Pta-F1, the last 352amino acids in the case of Pta-F2, and the last 470 aminoacids in the case of Pta-F3 (Fig. 2).PCR reactions performed in ... FEBSResultsExpression and purification of E. coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E. coli Pta, three conserved domains can be detected[Conserved ... islocated in the C-terminal domain, the E. coli PtaN-terminal domain is involved in stabilization of thehexameric native structure, in expression of the max-imum catalytic activity, and in allosteric...
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Từ khóa: chuyên đề điện xoay chiều theo dạngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Chuong 2 nhận dạng rui roTranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam