Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
... reduce catalytic activity of a cold-adapted alkaline phosphatase. Biochim Biophys Acta1774, 679–687.32 Janeway CML, Xu X, Murphy JE, Chaidaroglou A &Kantrowitz ER (1993) Magnesium in the active ... stability of Vibrio alkaline phosphatase, gave a coinci-dental reduction in mobility of a nearby spin-label located at C67, as deter-mined by EPR spectroscopy. This suggests that movement of ... distance of 12 A ˚. We demonstratethat EPR spectra of spin-labeled variants can be usedto extract information on local dynamics of the vari-ous secondary backbone structures and some tertiaryinteractions...
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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... kineticparameters Km, Vmax and kcatwere determined for thepurified AATB3. Values for Km and Vmaxfor bothamino donors (l-aspartate and l-glutamate) and ac-ceptors (a- ketoglutarate and oxaloacetate) ... mML-aspartatewas used as amino donor for a- ketoglutarate, and 30 mML-gluta-mate was used as amino donor for oxaloacetate. The activity of a- ketoglutarate was adjusted to 100.Identification of ... above, and a- ketoglutarate and oxaloacetate were used as amino acceptors. At the end of the reaction, 10 lL of the reaction solution was spottedonto a filter paper and separated by chromatography(n-butyl...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... Purification and characterization of a sialic acid specific lectin fromthe hemolymph of the freshwater crabParatelphusa jacquemontiiMaghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. Jeya SuriyaDepartment ... animal glycoconjugate. They actas an important component of the ligands recognized by thelectins. Recognition can be affected by specific structural variations and modifications of sialic acids and ... Denmark.47. Kamiya, H. & Ogata, K. (1982) Hemagglutinins in the acornbarnacle Balanus (Megabalanus roseus): purification and char-acterization. Nippon Suisan Gokkaishi 48, 1427.48. Kamiya,...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... of AF499 was identified asan archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence()35 to )23, AAANNNTTATATA) ... with anapparent molecular mass of 53 kDa appears as a double band inunboiled samples (lanes A1 and B1).Table 1. N-Terminal sequences of the polypeptides of the purified enzyme. N-Terminal sequen ... rmediate of the catalytic cycle. Hence, Hdr and the A. fulgidus enzyme not only share sequence similarity, butmay also have a similar active site and a similar catalyticfunction.Keywords: Archaeoglobus...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... bp wasproduced by PCR amplification with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAACAAACACAC-3¢, reverse primer: 5¢-AAGATGGTATTGAAGATGATGGTTGA-3¢), purified from agarose ... DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAAGGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGGTCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGTCATGACCTCG-3¢. One strand of each oligonucleotide ... Extraction kit (Qiagen, Valencia, CA, USA) and randomly labeled with32P using a Metaprime kit (Amer-sham Pharmacia Biotech Inc., Piscataway, NJ, USA). ForBAC DNA sequencing, the BAC clone was...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... compo-nents of each reaction mixture, after their chromatographicseparation and purification, were submitted to1HNMR(nuclear magnetic resonance) and FABMS (fast atombombardment mass spectrum) analyses ... Theconcentration of both the assayed compound and itsrespective transformation product was determined by comparison of peak data with those obtained fromauthentic standards chromatographed at different ... S-Adenosyl-L-methionine:flavonoid 4¢-O-methyltransferase crude activity in healthy and inoculated tissues of the carnation cultivar ¢Novada¢.In each row, values followed by a same number of * are not statistically...
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Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx
... Dickinson and Co.) was usedto acquire and analyze data. A minimum of at least 10 000cells was analyzed.Computation of specific constitutive activity (SCA) and relative SCA (RSCA)Given that the transfection ... directed against the extracel-lular domain of the TSH receptor (NCL-TSH-R2, Novo-castra Laboratories Ltd, Newcastle, UK) (data not shown), and by FACS analysis using BA8, a monoclonal antibodydirected ... the cAMP and IP pathways. These data show thatan inactive form of the TSHr which is trapped inside a cellafter transfection is able to gain the membrane surface whencombined with an activated...
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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
... oyster CaM and CaLP. In the presence of calcium, oyster CaLP and CaM appeared as a single band with an apparentmolecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence ... 5¢-untranslatedsequence, an open reading frame consisting of 483 bp, a TGA stop, a 146 bp 3¢-untranslated sequence, and a poly (A) tail of 18 nucleotides. A putative polyadenyla-tion signals (AATAAA) is recognized at ... architectures and outstandingmechanical performance, have received a great deal of attention from many biology and materials scientistsin the past few decades [1]. Shells and pearls are allproducts of...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... from Catharanthus roseusSantosh Kumar, Ajaswrata Dutta, Alok K. Sinha and Jayanti SenNational Centre for Plant Genome Research, JNU Campus, Aruna Asaf Ali Marg, New Delhi, IndiaCatharanthus ... database, i.e.Avicennia (BAB16317), Nicotiana secretory peroxidases (AAD33072), cotton (COTPROXDS) (AAA99868), barley grain (BP1) (AAA32973),Ar. thaliana (ATP 2A) A2 (Q42578) and HRP-C (AAA33377). ... primers GSTPF2 (5¢-GGAATTCCCATGGCTTCCAAAAC) and PGFPR1 (5¢-GGACTAGTATGTAACTTATTAGCT-ACATAT) using Deep VentRDNAPolymerase (NEB). The amplified product contains NcoI and SpeI restriction enzyme...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... 2007)doi:10.1111/j.1742-4658.2007.05728.x A highly active glutathione S-transferase was purified from adult Germancockroaches, Blattella germanica. The purified enzyme appeared as a singleband of 24 kDa by SDS⁄ PAGE, and had a different ... 28,321–329.26 Jirajaroenrat K, Ponjaroenkit S, Krittanai C, Prapant-hadara L -A & Ketterman AJ (2001) Heterologousexpression and characterization of alternatively splicedglutathione S-transferases ... D1 and D21. J Biol Chem 269, 27876–27884.23 Ranson H, Prapanthadara L -A & Hemingway J (1997)Cloning and characterization of two glutathioneS-transferases from a DDT-resistant strain of Anophelesgambiae....
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