Báo cáo khoa học: Regulation of secretases by all-trans-retinoic acid pot

... et al. Regulation of secretases by retinoic acid FEBS Journal 276 (2009) 2645–2655 ª 2009 The Authors Journal compilation ª 2009 FEBS 2655 Regulation of secretases by all-trans-retinoic acid Anna ... 42levels is mainly achieved by modulation of secretases, namely by the induction of a-secretase activity, by inhibition of b -secretases and ⁄ or c -secretases, or by a combination of both.This study ... the regulation of all secretases mediated by all-trans-retinoic acid (ATRA), and supports the hypothesis that ATRA iscapable of regulating them in an antiamyloidogenic sense at the levels of transcription,...

Báo cáo khoa học: Regulation of DNp63a by tumor necrosis factor-a in epithelial homeostasis docx

... inthe regulation of epithelial cell death. The degradation of DNp63a by TNF-a was delayed when cells were treated with nuclear factor-jB inhib-itors, whereas the induction of apoptosis by TNF-a ... destabilizesDNp63a by both proteasomal and caspase-dependentdegradation pathways. The degradation of DNp63a by TNF-a was attenuated by inhibition of NF-jB, sug-gesting that activation of NF-jB may ... DiRenzo J(2003) Positive and negative regulation of deltaN-p63promoter activity by p53 and deltaN-p63-alpha contrib-utes to differential regulation of p53 target genes. Onco-gene 22, 7607–7616.14...

Tài liệu Báo cáo khoa học: Upregulation of DR5 by proteasome inhibitors potently sensitizes glioma cells to TRAIL-induced apoptosis doc

... cleavage of procaspase-8 andprocaspase-10, and subsequent downstream activa-tion of the extrinsic and intrinsic pathways of apoptosis[4].Despite the considerable interest raised by the poten-tial ... cells.PIs potently induce expression of DR5in glioma cellsTo elucidate the underlying molecular mechanisms of the observed synergistic effects of PIs, we focused onthe transcriptional activation of ... activa-tion of DR5 after MG132 treatment was not affected by the knockdown (Fig. 5A). Furthermore, theknockdown of CHOP did not result in a signiﬁcantattenuation of apoptosis induced by TRAIL...

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... decapeptidesoverlapping the amino acid sequence of mature cathep-sin B (Fig. S1) were synthesized on the spots of cellu-lose membrane. After incubation of the membranewith 2A2 mAb, followed by the detection ... thepresence of various concentrations of 2A2 mAb. Thebinding of 2A2 mAb was followed by native gel elec-trophoresis. Increasing the concentration of 2A2 mAbat a ﬁxed molar ratio of cathepsin ... deter-mined using the SPOTs System and its associated software(spotsalot) according to the manufacturer’s instructions.Thirty-six overlapping decapeptide amino acid sequences Regulation of cathepsin...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... with a stochiometry of 1 : 1 of dTTP bound per subunit of dCTPdeaminase.Mutational analysis of amino acid residuesinvolved in dTTP regulation of dCTP deaminaseThe design of the mutant enzymes ... analyse the roles of His121 and Val122in catalysis and regulation of dCTP deaminase.Removal of the imidazole ring in H121A was anticipa-ted to relieve or reduce inhibition by dTTP by prevent-ing ... DenmarkSynthesis of dTMP by thymidylate synthase proceeds by the reductive methylation of dUMP, which isobtained via one of two parallel pathways. One path-way, considered to be a minor supplier of dTTP [1–3],involves...

Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... Inhibition of the ATPase activity was then explained by a slowingdown of the formation of active complex in solution. On theother hand, the acceleration of the sliding velocity (and thelower number of ... properties of an additional naturalcomponent of the thin ﬁlament framework, nebulin.Nebulin spans the entire length of thin ﬁlaments and iscapable of modulating the r ate of formation of the a ... gel. Densitometric analysis of the scanned gels w as performed usingMETAMORPH6.1software.ResultsLocalization of T800 within the I-band region of skeletal titinSome of us have previously demonstrated...

Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

... in the regulation of apop-tosis [21,22]. Thus, the possibility that the antiviraleffect of spermine is mediated by induction of apop-tosis was analyzed. For this purpose, the effect of spermine ... view of regulation of geneexpression by double-stranded RNA-mediated cell sig-naling. J Biol Chem 30, 30.35 Shah N, Thomas T, Shirahata A, Sigal LH & ThomasTJ (1999) Activation of nuclear ... Moreover,expression of a constitutively active form of IRF-3leads to induction of spermine, which possesses pro-apoptotic and antiviral activities. These results thusillustrate a potential new mechanism by...

Báo cáo khoa học: Regulation of calpain B from Drosophila melanogaster by phosphorylation pot

... understanding of the regulation of calpain B. According to homolo-gous modeling, the amino acid residues phosphory-lated by PKA and the ERK isoforms are situated insensitive regions of the molecule ... lowstoichiometry of phosphorylation in the case of PKA,and by the existence of multiple phosphorylation sitesin all cases. To assess the contribution of a well estab-lished site to the regulation of calpain ... wasstarted by the addition of CaCl2, to give the free concentra-tions of 50, 100, 350 and 2000 lm. The reaction was termi-nated by adding 15 lL of the reaction mixture to 5 lLof4 · concentrated...

Báo cáo khoa học: Regulation of luteinizing hormone receptor mRNA expression by mevalonate kinase – role of the catalytic center in mRNA recognition potx

... chains of Glu193and Ser146 help to stabilize the transition state of thec-phosphate group of ATP by magnesium ion. Lys13is also found to be within 3–4 A˚ of the C5 hydroxylgroup of mevalonic acid. ... studies have shown that binding of MVK to LHR mRNA suppresses the translation of mRNA in vitro [14]. As the mutation of any of thefour amino acids at the active site of MVK decreasesits binding ... ligand-induced downregulation of LHR is paralleled by aspeciﬁc, transient disappearance of its mRNAtranscripts [7,10]. Further studies on the mechanism of rapid degradation of LHR mRNA led to...

Báo cáo khoa học: Regulation of translational efﬁciency by different splice variants of the Disc large 1 oncosuppressor 5¢-UTR potx

... total amount of mRNA but also by the propor-tions of the different 5¢-UTRs within these messages,allowing the ﬁne tuning of DLG1 expression accordingto the physiological requirements of the cell.Materials ... analysisThe statistical signiﬁcance of the data from the luciferaseassays was obtained by analysis of variance (ANOVA), fol-lowed by multiple comparisons performed by the Duncantest. A P value < ... Amenitsch H &Banks L (2000) Differential regulation of human papil-lomavirus E6 by protein kinase A: conditional degrada-tion of human discs large protein by oncogenic E6.Oncogene 19, 5884–5891.15...

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