Báo cáo khoa học: Low U1 snRNP dependence at the NF1 exon 29 donor splice site doc

... and mutated exon 29 to generate fragments that contain the exon along with the intronic flanking sequence, using the following oligonucleo- tides: NF29-F, 5¢-ttcattcatatgaccatttgaatatacaatggt-3¢; ... interaction between U1 snRNP and 5¢SS FEBS Journal 276 (2009) 2060–2073 ª 2009 The Authors Journal compilation ª 2009 FEBS 2073 Low U1 snRNP dependence at the NF1...

Ngày tải lên: 16/03/2014, 01:20

... This indi- cates that, with guanine as a substrate, the K m value is approximately four-fold higher, and the V max value is approximately 310-fold lower compared to HPRT. Therefore, the catalytic ... be responsible for the low activity observed by acting as a weak base. The data obtained in the present study indicate that PRTFDC1 does not have a direct catalytic role in the nu...

Ngày tải lên: 15/02/2014, 01:20

... auto-oxidation rates of Ec DOS PAS, and catalytic activities of full-length enzymes. We found that the mutations at Asp40 markedly altered t he redox potential and auto-oxidation rate. Fur- thermore, ... using the Quikchange Site- Directed Mutagenesis Kit TM (Stratagene) with the following oligonucleotides: Asp40Ala: 5¢-TGTTAATTAACGAAAATGC TGAAGTGATGTTTT TC-3¢ (forward); 3¢-GAAAAACAT...

Ngày tải lên: 19/02/2014, 16:20

... S28053-1, showed that the acetate leaving group at the C1 position is the main reactive site in the drug: a reaction scheme common to GSH and guanines is presented. At the cellular level, the presence ... acetate, pH 8.0, and analyzing the sample at the various time-points specified in the figure legend. For the alkylation reactions, the various GSH derivatives (10...

Ngày tải lên: 20/02/2014, 11:20

... anionic site, E2020 can either bind at the active site or at the peripheral anionic site. The observed dependence of the inhibitory potency of a series of N-benzylpiperidine benzis- oxazoles on the ... that E2020 interacts with the active -site and the peripheral ani- onicsiteinAChE,butinthecaseofBChE,asthegorgeis larger, E2020 cannot simultaneously interact at both...

Ngày tải lên: 30/03/2014, 20:20

... the concentration of the reduced cosubstrate NADPH. For isomerization of fructose 6-phosphate, phosphoglucoisom- erase was added. Mathematical modelling, parameter identification and simulation A mathematical ... interdependencies rather than simple steady-state concentrations. We present a method based on dynamic modelling that allows for the quantification and visualization of cellular...

Ngày tải lên: 14/02/2014, 22:20

... paper focuses on the exploration of term dependence in the application of sentence retrieval. The adjacent terms ap- pearing in query are assumed to be related with each other. These assumed ... define the styles of term dependence in query. In this paper, the assumption of term dependence starts by consid- ering the possible syntactic relationships of terms. For that...

Ngày tải lên: 08/03/2014, 02:21

... of the mPTP [45]. Thereby oxidation of SH-groups of the adenine nucleotide translocator stimulates pore open- ing. We hypothesize that elimination of ROS may reduce the permeabilization of the ... control conditions, that is, incubation of mitochondria in Ca 2+ - free and air saturated medium in the presence of 5 mm glutamate and 5 mm malate, oxidative phosphorylation was well coupl...

Ngày tải lên: 16/03/2014, 22:20

... glosses. The results are presented in the plot of Figure 1. We compute three variants separately, by considering the use of the in-domain data: only for the translation model (TM), only for the language ... Impact of the size of in-domain data on MT system performance for the test set. to avoid the possible effect of over-fitting we focus on the behavior on the test set....

Ngày tải lên: 23/03/2014, 18:20

... from the dataset, and cross validation was performed over the residue, including the shorter strings in the training data (i.e. TM) on each iteration. In N-fold stratified cross validation, the dataset is ... simply outputs these for manual manipulation by the user in fashioning the final translation. A key assumption surrounding the bulk of past TR research has been that the...

Ngày tải lên: 23/03/2014, 19:20