Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc
Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc
... structures of the wild-type, single- and double-mutant EhPGKs in aclosed conformation will certainly help to clarify the molecular arrangement in the guanine ring binding siteduring the binding of ... carbon 2 of the guanine ring may interact with the side chain of Glu309, whereas the carbonyl group atposition 6 of the guanine ring may interact with the hydroxyl group of the Tyr239 side chain (Figs ... kinase gene for the correspondingamino acid residues present in the adenine nucleotide-dependent phospho-glycerate kinases and the recombinant proteins were purified. Kinetic anal-ysis of the...
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
... solubleversion of the mutant protein. The Kcat⁄ Kmvalue of the mutant TNSALP was less than one-tenth of the Kcat⁄ Kmvalue of the soluble form of the wild-typeenzyme, indicating that the replacement ... resulting in a Kcat⁄ Kmvalue that was < 10% of that of the wild-type enzyme (Table 1), indicating that the conversion of valine to alanine at position 406 in the crowndomain compromises the catalytic ... 3B),further supporting that the folding and assembly pro-cess and subsequent intracellular trafficking of TNSALP (V406A) are largely normal in the stable cellline.Kinetics of the soluble form of...
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Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc
... An in silico dockinganalysis of glyphosate binding at the GO active siteshowed that glyphosate is bound in the same orienta-tion as inferred for glycine (with the phosphonate moi-ety pointing ... in the binding energy for this smallsubstrate. Because of the introduction of an arginineat position 54, the a2–a3 loop (comprising resi-dues 50–60) assumes a different conformation in the G51S ... (blue) structures shows the dif-ferent conformations of the main chain of the a2–a3 loop, seearrows [49]. For the sake of clarity, only the FAD and the ligandbelonging to the wild-type GO structure...
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Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx
... novel inter-action surfaces for two distinct molecular partners [6],and the conformation of a proline in the linker regionbetween two SH3 domains of the Crp adaptor proteindetermines the autoinhibition ... detected in vitro of CypA on Pro90 in the full-length Gag protein [5] plays a role in the viralrestriction process in vivo remains an open question. In the case of the Itk kinase, the cis ⁄ ... and the FK506-binding proteins(FKBPs), in the protein-folding process, where the con-formational state of a prolyl peptide bond can be arate-limiting step [1]. The importance of ‘binding ver-sus...
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Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot
... to the Golgi for further glycosylation(p2 form with molecular mass of 69 kDa). The p2 form of CPY traverses the Golgi and is packaged into vesi-cles destined for the vacuolar protein sorting ... motif for pro-tein–protein interaction. In this study, Osh6p wasdemonstrated to bind a pool of phosphatidylinositolphosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P,PtdIns(3,4)P2and PtdIns(3,5)P2with ... Asshown in Fig. 7, at 0 min of chase, p1 CPY was the predominant form. The p2 form was seen at the 5 mintime point. At a later time (10 min), mature CPY wasobserved together with some remaining...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... sites within the MGP coding sequence(Fig. 2 ). Analysis of the phase of each of the xMGP intronslocated within the coding region revealed that introns 2 and3 are of phase I while intron 4 is ... is split into two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localizedwithin the 5¢ UTR region of t he xMGP gene (Fig. 1 andTable 2 ). The other introns (2, ... DNA-binding. In order to delineate the cis-regulatory sequences involved in mediating transcriptional activation of the xMGP gene,we engineered several promoter constructs involving 5¢ andinternal...
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Báo cáo khoa học: Molecular basis of toxicity of Clostridium perfringens epsilon toxin ppt
... compro-mises the intestinal barrier of intoxicated animals,allowing the dissemination of toxin via the bloodstreamto the main target organs of the kidneys and brain. The mechanism of e-toxin absorption ... that toxin-inducedoedema of the brain is due to the damaging action of the toxin on vascular endothelial cells [45]. Toxin-induced increase in vascular permeability in the brainwas initially ... without poreformation. The final step of pore formation mightinvolve unfolding of the amphipathic hairpin and itsinsertion into the membrane to form the walls of the pore composed of 14 b-strands.Prevention...
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Báo cáo khoa học: Molecular basis of cytokine signalling – theme and variations docx
... N-terminal extension (clip) binds into the epitope for the type I receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMPligand. Therefore, ... abinding site for the tyrosine kinase Janus kinase 3(JAK3), which transactivates. The driver, IL-4Ra,contains a large cytosolic domain with binding motifs for Janus kinase 1 (JAK1), the intracellular ... Step 1 is the binding of the soluteligand at the high-affinity site and in step 2 the secondchain is recruited in the membrane to form the signal-ling oligomer. The cytosolic parts of the homodimercarry...
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Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf
... the deformability required for the formation of the Early protein 2 C-terminal DNA-binding domain–DNA complexes of var-ious types is based not only on the rigidity of the base sequences in the DNA ... tochanges in the DNA recognition kinetics and in the stability of the complex [22], suggesting that there isan involvement of this loop in HPV-16 E2–DNArecognition.Another interesting aspect of HPV-16 ... representedby the presence of Lys349 in the loop connecting helixa2 and strand b4. This residue may have a role in DNA binding, as a single mutation of Lys349 to alan-ine weakens the DNA binding of the...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... that the failure of these seven mutations toabolish protein phosphorylation was a result of otherprotein kinases masking the PKA-catalysed part.Indeed, protein kinases other than PKA, includingtyrosine ... assess the role of the S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions of the other subunits in a het-erooligomer, the corresponding mutations ... reduced when these three amino acidswere mutated. The data obtained in the present study provide informationabout the structural determinants that are responsible for protein kinase Aphosphorylation...
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