Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf

... following results: a-1 antitrypsin, transthyretin and a-1- acid glycoprotein showed strong interactions with preprohepcidin. We further confirmed and examined the a-1 antitrypsin binding in vitro (glutathione S-transferase, ... MALDI-TOF) and in vivo (ELISA, cross-linking assay). Our results demonstrated that the serine protease inhibitor a-1 antitrypsin binds pre...

Ngày tải lên: 16/03/2014, 01:20

... and pen input, includ- ing both pointing and handwriting (Figure 1). Our application task also differs, focusing on search and browsing of a large database of movies-on- demand and supporting ... focus and select items. The pen can be used both for selecting items (pointing at them) and for handwritten input. The graphical user interface has three main screens. The main...

Ngày tải lên: 20/02/2014, 12:20

... solid line), in the presence of 100 l M neurotoxin ( • , dotted line), in the presence of 200 l M neurotoxin ( , dotted line) and in the presence of neurotoxin and 50 l M dopamine (h, dashed line). Modulation ... chromatography [18]. The eluates were pooled and the amount of protein was determined by the bicinchoninic acid assay [38] using bovine serum albumin as a s...

Ngày tải lên: 14/02/2014, 19:20

... a protein belonging to the GTP- binding elongation factor family, which promotes the GTP- dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and the capture ... sequence, increasing the number of basic residues of lysine, arginine and histidine, could occur. However it seemed probable that the eEF1A isoforms could act as a controlling ev...

Ngày tải lên: 21/02/2014, 00:20

... c-tubulins are also candidates for adaptive compensation. Amino acid changes adjacent to the T3 loop and within the T5 loop may in uence the binding affinity and hydrolysis of GTP and ⁄ or the egress ... involved in contacts with the bound GTP [37]. Residues involved in longitudinal contacts at the ‘plus’ and ‘minus’ ends are indicated by ‘+’ and ‘)’, respectively, w...

Ngày tải lên: 07/03/2014, 04:20

... four kinetic phases in the reduction of the hemes by menaquinol. According to the quinone type used, the two hemes of the cytochrome are probably involved in the transfer of electrons from the ... use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH 2 at the Q P site [27]. Study of the reduction and oxidation of the cytoch...

Ngày tải lên: 07/03/2014, 15:20

... expressing the PCho-containing glycoforms and that OS elongation pro- tects the organism from killing mediated by naturally acquired antibody and complement. In several H. in uenzae strains, the ... observed corresponding to glycoforms containing only one PEtn group or containing one PEtn group and a phosphate group. In order to obtain sequence and branching information, t...

Ngày tải lên: 08/03/2014, 02:21

... on the generation of annotation and metadata databases in order to in- crease the usability of these components of the AMI Corpus. In the following sections we describe the problem, present the ... metadata using the Open Archives network will contribute to increase the visibility of the AMI Corpus. 1 Introduction The AMI Meeting Corpus (Carletta and al., 2006) i...

Ngày tải lên: 08/03/2014, 02:21

... tail and the transmembrane region were deleted from the rat isozyme, and an insulin signal sequence and a Protein A-IgG binding domain were fused to the resulting N terminus of the sequence. The ... of the plasmid pGIR201protA (a gift from H. Kitagawa, Kobe Pharmaceutical University) [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG...

Ngày tải lên: 08/03/2014, 10:20

... of inosine is the intracellular pool of adenine nucleotides, (b) the decrease in ATP and the increase in inosine, promoted by H 2 O 2 , cannot be explained solely by a change in the level of the ... resuspended in fresh medium (without H 2 O 2 ) and incubated further for 120 min. At the times indicated, the adenylic charge, ATP and inosine were determined as descri...

Ngày tải lên: 17/03/2014, 10:20