Báo cáo khoa học: Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein potx

... freezing the samples. Assessment of Ab fibril formation on the basis of ThT fluorescence The presence of b-sheet structures and the kinetics of fibril formation were monitored by means of ThT fluorescence spectroscopy. ... lg of synthetic Ab monomer was incu- bated in fibril formation buffer in the presence of 0.2 l gof sonicated Ab fibrils in a total vol...

Ngày tải lên: 06/03/2014, 01:20

... ª 2009 FEBS 2011 Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein Erik Lundberg 1 , Anders Olofsson 2 , Gunilla ... with the thermostability of its tetrameric and monomeric structures. Thermostability and protein structures We have analyzed the structures of hT...

Ngày tải lên: 16/03/2014, 01:20

... control both syn- thesis and activity of nitrogenase in response to o xygen and nitrogen availability, because of the high energy demands of nitrogen fixation and the oxygen sensitivity of nitrogenase [1,2]. ... conditions. Protein synthesis of approximately equivalent amounts of all three proteins was induced from plasmid pRS209, which contains the operon malE-nifLA...

Ngày tải lên: 07/03/2014, 16:20

... after injection. Moreover, the exclusion of the protein in urine and faeces, and uptake by the liver, was relatively low. On the other hand, the least stable human CD69NS84 protein dis- played a rapid ... biochemical stability. Interest- ingly, the stability of the short proteins CD69NV82 and CD69NS84 is much lower using these criteria, both from the point...

Ngày tải lên: 16/03/2014, 04:20

... at the herd level. They perceive the collection of the data in and of itself as the basis for taking relevant action at the farm. They may skip the process of systematic anal- ysis of data and ... understanding with regard to decision levels Based on analysis of the veterinarians' perceptions of how they wished to use the metritis score in their practice...

Ngày tải lên: 25/10/2012, 10:45

... in terms of its ability to enter the cells of the retina, the present study used reti- nal pigment epithelial (RPE) cells as a model to deter- mine the susceptibility and the mediators of HSV-1 entry ... nec- tins induce formation of filopodia and lamellipodia through the respective activation of Cdc42 and Rac small G proteins. J Biol Chem 277, 50749–50755. Suppor...

Ngày tải lên: 18/02/2014, 14:20

... synthase. The putative genes were of two types, either with the two-domain architecture of the RibG protein of Bacil- lus subtilis or the RibD protein of E. coli, respectively [18–20], or the single-domain ... catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1¢ (S) position of the substrate. These results are in agreement with t...

Ngày tải lên: 18/02/2014, 18:20

... cisplatin and the ability of the particular p53 target site to accommodate the cisplatin IACs. The higher the probability of formation of the cisplatin IACs within the p53DBSs due to the occurrence of the ... weights of the p73 isoforms. To verify the specificity of the band shifts for DNA complexes with the proteins studied, we used the band supersh...

Ngày tải lên: 19/02/2014, 05:20

... and retained the binding efficacy. The flexibility of the target molecule complicates the prediction of an effect caused by a modification on the inhibitor and necessitates structural analysis of ... 2004 Structure of the complexes The crystal structures of the six protease–inhibitor com- plexes have been solved and refined down to 1.8–2.3 A ˚ resolution with R cry...

Ngày tải lên: 19/02/2014, 16:20

... (Fig. 3Dc). These results, together with the results of RT-PCR and western blot (Fig. 1), implies that the expression of sPLA 2 -IIF in RA is rather lower than that of other sPLA 2 s and does not ... sPLA 2 to evaluate the expression and localization of six sPLA 2 s (IIA, IID, IIE, IIF, V and X) in human joints affected by RA. We further examined the possible contrib...

Ngày tải lên: 19/02/2014, 16:20