Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... compilation ª 2009 FEBS UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated Amrita Brahma*, Nupur Banerjee* and Debasish Bhattacharyya Structural ... in exploring the regulatory behavior of this enzyme. To avoid misinterpretation of the results, the composition and stoic...

Ngày tải lên: 16/03/2014, 00:20

... excluded from the regression, because they are very different in the exponential and stationary phases. ATP and energy charge were excluded because for some of the samples from the cultures of overpro- ducing ... characterization of the wild-type In the exponential phase of the ammonium cultures all of the PPP enzyme activities were lower and the NADP...

Ngày tải lên: 07/03/2014, 17:20

... transfer of one electron to the hemes, then to the [Fe-S] cluster, to be finally accumulated at the level of the molybdenum cofactor to be able to undertake the catalytic reaction. The location of the ... site of fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH 2 at the Q P site [27]. Study...

Ngày tải lên: 07/03/2014, 15:20

... second was the ambiguity that some matrix S-initial NPs are actually dependents of the VP head (in these cases, NEGRA annotates the finite verb as the head of S and the non-finite verb as the head of VP). ... dislocations of all sorts are expressed at the S and VP levels in CFG parse trees, such as VP- 1 of Figure 2, but many adverbial and subordinate phrases...

Ngày tải lên: 08/03/2014, 04:22

... mice [28]. The instability of DA at the neutral pH of the cytosol causes the forma- tion of toxic aminochromes, and these species are not formed when DA is protonated at the acidic pH of the storage ... counter. Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the...

Ngày tải lên: 14/02/2014, 21:20

... in blue (drawn from PDB code: 1sb0). Positions of E665 and E555 of the CBP–KIX domain, and residues K291 and R294 of the c-Myb transactivation domain are indicated. (C) The CBP–KIX:cMyb:MLL1 ... 2AGH). The MLL1 TAD is shown in green and the colors for the CBP–KIX:cMyb are as in (B). Upon formation of the ternary complex, residues E665 and E666 of the...

Ngày tải lên: 16/02/2014, 14:20

... neurodegenerative disease. Synthesis and secretion of GIF in the brain Cellular and temporal localization of GIF synthesis Whilst the MT1 and MT2 isoforms are ubiquitously synthesized throughout most ... role of GIF in the pathogenesis of AD, and therefore we will briefly review some of the current thoughts on the role of GIF in AD. There are numer- ous pathol...

Ngày tải lên: 16/02/2014, 15:20

... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spectra were used to calculate the fraction of Pyr-ONC (M23L), ... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance o...

Ngày tải lên: 19/02/2014, 12:20

... deposition in the airways of Der p 2-sen- sitized and nonsensitized mice. The fate of the allergen could be followed in vivo both at the whole-body level and at the molecular level, through the application ... shown that the turnover of airway dendritic cells is influenced by the inflammatory state of the lungs [24] and that these cells play an essential role bot...

Ngày tải lên: 07/03/2014, 21:20

... full-length D02; c and d, DNS ⁄ 28 8–3 06, e and f, g and h, and i and j, N-deletion DNS mutants 29 2–3 06, 29 3–3 06, and 29 6–3 06, respectively; k and l, C-deletion DNS mutant 28 8–3 03. Upper and lower panels ... proteins and HuR, are imported into the nucleus by an mRNA synthesis-dependent process and they shuttle continuously between the nucleus and...

Ngày tải lên: 07/03/2014, 21:20