Báo cáo khoa học: Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 ppt

... ª 2009 FEBS 6083MINIREVIEW Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 Nadja Bitomsky and Thomas G. HofmannGerman Cancer Research ... PMLFig. 1. Regulation of DNA damage- induced cell death by p53 and HIPK2. Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated protein kinases ATM and ATR. ATR and ATM ... between p73 and HIPK2, as previously described for HIPK2 and p53, also remains to be clariﬁed.Post-translational modiﬁcations of p73 by acetyla-tion through p300 and by phosphorylation by the DNA damage- activated,...

Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING ﬁnger protein Unkempt doc

... activa-tion of the G protein p21 Rho by deamidation of glutamine. Nature 387, 72 9–7 33.24 Lanning CC, Ruiz-Velasco R & Williams CL (2003) Novelmechanism of the co -regulation of nuclear transport of SmgGDS ... ubiquitina-tion is of the degradative type and could regulate the level of BAF60b inSWI ⁄ SNF complexes. Our observations support the new idea of a directconnection between Rac signalling and chromatin ... between these transcription factors and SWI ⁄ SNF complexes, thereby allowing the recruit-ment of SWI ⁄ SNF to target genes [ 5–9 ].Biochemical puriﬁcation and analysis of SWI ⁄ SNFcomplexes have revealed...

Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 pot

... least inthe absence of an activating adaptor, generation of active human caspase-2 requires separation of its large and small subunits. In the context of autoactivation,removal of the prodomain ... approach deﬁnes speciﬁcities of members of the caspase family and granzyme B. Functional rela-tionships established for key mediators of apoptosis. J Biol Chem 272, 1790 7–1 7911.6 Bergeron L, Perez ... c release and apoptosis from the nucleus. J Biol Chem 277, 1514 7– 15161.10 Guo Y, Srinivasula SM, Druilhe A, Fernandes-AlnemriT & Alnemri ES (2002) Caspase-2 induces apoptosis by releasing...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... 3 450 – –+ + +–+ – – – ++ +++ + –+ Fig. 9. GRASP55 is important for MT1-MMP–furin complex formation and activation of pro-MT1-MMP. (A) Lysates of bbHT1080 cells trans-fected with pCDNA3.1 ... then lysed and the extract wasA605040IB: MT1-MMPMT1-MMPpCDNA3.1 Zeo+kDa +– –+ +– –+ 605040IB: GRASP555040IB: β-actin12IgGanti MT1-MMPB605040IB: GRASP551 – – – ++++2kDaGRASP55FMT1/MYCIP: ... MYC50 +– – – – – WB: MYCWB: FLAGInput lysates123456060Fig. 5. The LLY motif in the MT1-MMP ICDis crucial for the interaction with GRASP55.(A) Interaction between GAL4-GRASP55 and VP16-MT1...

Tài liệu Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage pdf

... TRAIL-induced apoptosis by CCCP. OncolRep 18, 7 1–7 6.37 Park SY, Billiar TR & Seol DW (2002) Hypoxia inhibi-tion of apoptosis induced by tumor necrosis factor-related apoptosis- inducing ligand (TRAIL). ... chon-drocyte apoptosis and may suppress the progression of arthritis.Materials and methodsInduction of OABeagle dogs (n = 20) with a mean ± SD age of 1.4 ± 0.4 years and a mean ± SD weight of 10.2 ... a wide range of responses, depending on cell type and levels of ROSwithin the cell [23,24].The aims of this study were to investigate the major signalling pathways and effects of hypoxic conditionsin...

Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

... plants and a variety of enzyme reactions includingcertain dehydration and isomerisation reactions [ 1–3 ].In view of the vital role of these coenzymes, it appearslikely that biosynthesis of the ... units of guanylate kinase and 2 units of pyruvate kinase in a totalvolume of 0.5 mL, and were incubated for 30 min at 37 °Cin order to generate the reductase substrate 3 (the addition of guanylate ... undergo hydrolytic cleavage of the position 2 amino group by adeaminase (in plants and most eubacteria) or reduction of the ribose sidechain by a reductase (in fungi and archaea). We compare 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone...

Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... [2,3]. The lig- and for the tandem GAF domains of PDE2, PDE5,PDE6, and PDE11 is cGMP and that for PDE10 iscAMP. Usually ligand-binding enhances catalyticactivity [ 4–6 ]. Two ACs of the cyanobacterium ... structures of the tandem GAFdomains of PDE2 and cyaB2 do not reveal how cNMP speciﬁcity is enco-ded, we generated chimeras between the tandem GAF domains of cyaB1 and PDE2. Both bind the ligand in ... cyano-bacterial amino acids I250–I283. For construct II (Table 1),the region between BssHII and SacI (I250–G267 of cyaB1)was replaced by N411–N426 of rPDE2A. By transfer intothe pQE30 expression...

Tài liệu Báo cáo khoa học:Insulin-like growth factor 1 signaling regulates cytosolic sialidase Neu2 expression during myoblast differentiation and hypertrophy doc

... activation of theRas–Raf–Mek–Erk pathway and cell differentiation ⁄hypertrophy through activation of the PI3 kinase–AKT–mTOR pathway [9].Accordingly, IGF-1 treatment (5 ngÆmL)1) of C2C12myoblasts ... nmol of the substrate 4-methylumbelliferyl N-acetylneuraminicacid (Sigma-Aldrich), 100 lg of BSA and aliquots of cytoso-lic fractions (5 0–1 00 lg of proteins) in a ﬁnal volume of 0.2 mL of 50 ... terminated by addi-tion of 0.8 mL of 0.25 m glycine buffer (pH 10.4), and theamount of 4-methylumbelliferone released was determinedﬂuorometrically with an excitation wavelength of 365 nm and an...

Tài liệu Báo cáo khoa học: "An ERP-based Brain-Computer Interface for text entry using Rapid Serial Visual Presentation and Language Modeling" ppt

... be a randomly-ordered set of all theletters (and the space and backspace symbols). Theletters are randomly ordered for each sequence be-cause the magnitude of the ERP, hence the quality of the ... manufac-tured by g.tec (Austria), has 24 bits of precision and has 16 channels. We use a Butterworth bandpass ﬁl-ter of 0.5 to 60 Hz, a 60 Hz notch ﬁlter, a samplingrate of 256 Hz, and we buffer ... ˆπcare the estimates of the class means and priors, respectively, x is the data vector to beclassiﬁed, and fN(x; µ, Σ) is the pdf of a multivari-ate normal distribution.The set of visual stimuli...

Tài liệu Báo cáo khoa học: The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC doc

... theEscherichia colimembraneindependent of the Sec translocase and YidCSandra J. Facey and Andreas KuhnInstitute of Microbiology and Molecular Biology, University of Hohenheim, Stuttgart, GermanyKdpD ... OmpA (upper panel) and KdpD (lower panel) and analysed by SDS/PAGE and visualized by phosphorimaging. Thepositions of the molecular weight standards (SeeBlueTMPre-StainedStandard, from Invitrogen) ... periplasmicloop of KdpD and a T7-epitope of the T7 major capsid protein into thesecond periplasmic loop of KdpD. (B) lists the amino acid sequences of each of the two extra-membrane loops before and after...

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