Báo cáo khoa học: Structural origins for selectivity and specificity in an engineered bacterial repressor–inducer pair pdf

... positioning of the ligands and the conformations of the residues lining the binding site (Table 2). In an initial comparison of the ligand positions of tetracycline, dox and atc in TetR, these ligands ... well as for all corresponding single and double mutant variants, are highly valuable for discussing the origins of speci- ficity and selectivity [8,9] (Table 3). When an...

Ngày tải lên: 16/03/2014, 00:20

... 2009 FEBS 3673 and His316. These residues are positioned within the loop linking b-strands C and D (His168 and Glu170) and on b-strand H (His316), indicating that the iron- binding facial triad ... undergoing interactions mainly with the C-terminal helical subdomain. Accordingly, VioC exerts broadened substrate specificity by accepting the analogs l-homoarginine and l-canavanine f...

Ngày tải lên: 16/03/2014, 02:20

... papain and actinidin, and on amino acid sequence information for cathepsins B and H, and stem bromelain. J Mol Biol 182, 317–329. 11 Carter CE, Marriage H & Goodenough PW (2000) Mutagenesis and ... of papain and ervatamin-A for residue 157 (papain numbering, 155 for ervatamin-A) and residue 67, respectively. Table 1. Kinetic constants for peptidyl p-nitroanilides an...

Ngày tải lên: 18/02/2014, 16:20

... and Asp245, to coenzyme binding and specificity in P. asaccharolyticus GDH. Five mutants were created: in E243K and E243R, the P7 glutamate was replaced by positively charged lysine and arginine, ... coenzyme binding was tighter and therefore measurable and, in particular, gave measurable K d values for binding of NADPH to both E243K and E243R, whereas the binding of NAD...

Ngày tải lên: 30/03/2014, 03:20

... domain. Stabilizing interactions could form between His230 of PP2A-C and Asp119 and Asn94 of Anp32a. A salt bridge could form between Glu226 of PP2A-C and Lys67, Lys68 and Lys91. Testing the interaction ... subnuclear structures. The interaction was mapped onto the LRR and AXH domain of Anp32 and Atx1 respectively, and was shown to be stronger for expanded Atx1 [16,18]. The te...

Ngày tải lên: 18/02/2014, 17:20

... maltodextrin-binding protein ( TvuCMBP). The bacterial sugar-binding protein is a member of the bacterial ATP-binding cassette transport system, and the mechanism of the maltodextrin transport in Escherichia ... proteins As the sugar-binding site is formed by N- and C-domains, residues involved in sugar binding are grouped into two parts, the N-domain part and the Table 1. Data...

Ngày tải lên: 19/02/2014, 00:20

... lgofc 1 purified in detergent, the slower migrating band reappeared (lanes 4 and 8). Upon incubation of 2 lg of pure c 11 with 2 and 10 lgofc 1 purified in chloroform ⁄ methanol, the slower migrating c ring ... c subunit, and in accordance with the binding change mechanism [3], this arrangement suggests a catalytic mechanism in which the c subunit rotates within the a 3 b 3 cylind...

Ngày tải lên: 07/03/2014, 21:20

... G28 or G37 and G38 in aptamer no. 18, as well as G25 and G26 or G39 and G40 in aptamer no. 20, in Co 2+ binding. Interestingly, a similar metal ion binding mode to the tandem guanines has been ... determined j values were very similar: 4.0 and 3.6 for G27 and G28 in aptamer no. 18 and 3.7 and 3.5 for G25 and G26 in aptamer Fig. 3. Iodine cleavage analysis of phos...

Ngày tải lên: 16/03/2014, 06:20

... Enzyme–MeGlcA 2 Xyl 3 interactions (for clarity, Ser258, forming a hydrogen bond to MeGlcA, is not shown). Ligands and amino acids involved in ligand binding are in ball -and- stick represen- tations (atoms: ... a) 8 -barrel domain is in red, the C-terminal b 9 -barrel domain is in blue, the con- necting region is in orange, the catalytic amino acids Glu165 and Glu253 are in...

Ngày tải lên: 22/03/2014, 16:20

... (2852 and 2923 cm )1 ) assigned to the symmetric and antisymmetric methylene stretching modes, and weaker bands (2875 and 2956 cm )1 ) assigned to the symmetric and antisymmetric methyl stretching ... of the mem- branes using an Alpha Innotech image analyser and the Alpha Innotech fluorchem 8800 (San Leandro, CA, USA) software. To differentiate between chemotaxis and chemo- ki...

Ngày tải lên: 23/03/2014, 07:20