Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... (labelled +2).(B) Cut-away view of L3 binding in the active site pocket showing the Phe-Phe clamp and the F229A mutation. Carbohydrate binding subsites are labelled )1, +1 and +2. Glycosidic bond cleavageoccurs ... how the substrate is initially recog-nized by the particular enzyme and then drawn into the active site. Clearly, a detailed understanding of the protein carbohydrate interactions that determinespecificity ... maps showingordered carbohydrate in the main sugar -binding sites, Fig. 2. Structural consequences of muta-tions at the Phe-Phe clamp of the +1 sub- site. Disposition of F144 (lower) and F258(upper)...
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Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx
... molecule binds at three subsites in the polysaccharide binding site of the TSbH domain of HylP2. The positions of ligandsare indicated at the substrate binding groove. (B) The view from the top ... molecules, indicating the strong nature of the binding of ascorbic acid to proteins. Althoughascorbic acid is a small molecule, it blocks the mostattractive binding subsites in the protein, leading ... examination of theseregions indicates that PheC175 protrudes into the sub-strate binding area between subsites 1 and 2. Thus, ithampers the attachment of ligand at this subsite. Simi-larly, the...
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Báo cáo khoa học: Autoregulatory binding sites in the zebrafish six3a promoter region define a new recognition sequence for Six3 proteins potx
... DNA- binding properties of murine Six3, in vitro binding site selection demonstrated high-affinity binding tosequences containing the TAAT core motif, and clus-ters of these tetranucleotide sites ... binding mode of the HD and in uence the nucleotide contacts made by the recognition helix [6].Notably, these different binding modes seemed todepend on the binding site sequence, and the binding affinities ... region showing the positions of sites contain-ing ATTA (and TAAT) core sequences.Fig. S2. Sites within the six3a promoter containingoppositely oriented TAAT cores that showed weak binding as compared...
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Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-affinity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx
... depicts the binding isotherm; open circles represent the integrated binding heat of the data in the top panel, and the fullline is the fit of a one -site binding model to the integrated binding data. ... 2011)doi:10.1111/j.1742-4658.2011.08043.xStarch -binding domains are noncatalytic carbohydrate- binding modulesthat mediate binding to granular starch. The starch -binding domains from the carbohydrate- binding module family ... &Gomez-Casati DF (2010) The starch -binding capacity of the noncatalytic SBD2 region and the interactionbetween the N- and C-terminal domains are involved in the modulation of the activity of starch...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... sequencelacking the PIN domain was obtained in two steps. First, the fragment of 423 bp situated in front of the PIN domainwas amplified by PCR using forward primer 5¢-CGGGATCCCGGAGTCTGAGCTATGAGTG-3¢ ... RNaseproperties of MCPIP are exerted by the PIN domain, asshown by experiments with mutated MCPIP (either lack-ing the PIN domain, or with two conservative aminoacid mutations within PIN domain). Interaction ... processes, such as actin. Further stud-ies characterizing proteins interacting with MCPIP willclarify the dynamics and features of this protein. Role of MCPIP in regulation of the endogenousIL-1b...
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Báo cáo khoa học: Short hydrogen bonds in proteins Sathyapriya Rajagopal and Saraswathi Vishveshwara pot
... amber. The classification based on sequence separationhelps in assessing the in uence of SHBs at the locallevel or at the level of sequentially separated spatialinteractions in the protein structure. ... [33,34]. The role of SSHBs in protein–ligand complexes hasbeen studied and the donor and acceptor furcation hasbeen analysed in detail [10], from the point of view of recognition of the ligand by the ... reported in several proteins and their implication in catalysishas been discussed in detail, we noticed that a system-atic analysis in terms of the nature of the donors-acceptors and their possible...
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Báo cáo khoa học: Insulin like growth factor-1-induced phosphorylation and altered distribution of tuberous sclerosis complex (TSC)1⁄TSC2 in C2C12 myotubes pptx
... evidence indicating that the phosphorylation of TSC2 at the S939 site comprises the primary recognition motif of 14-3-3 protein binding. Having established the importance of the S939 site in TSC2 ... collected at 10, 30 and 60 min after the initiation of IGF-1 stimulation. (A) Changes in phosphorylation states of Akt (T308 and S473 sites) and S6K1 (T389 and T421 ⁄ S424 sites) were determined. ... S473 sites, S6K1 at T389 and T421 ⁄ S424 sites, IRS-1 at S636 ⁄ 639 sites, PRAS40 at the T246 site and GSK3-a ⁄ b at S21 ⁄ 9 sites) . Each of the PI3K ⁄ Akt ⁄ mTOR targets was phosphory-lated...
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Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
... 2B).Because oxidative stress can induce uncoupling pro-tein 2 (UCP2) expression at the expense of ATP synthe-sis [28], we then determined the level of UCP2 protein and ATP production in RINm5F cells. ... (mitochondria) stain-ing; (C) anti-His (exogenous PHB)-FITC stain-ing; (D) merge. The arrows indicate staining of His-tagged PHB in (C) or both PHB and Mitofluor in (D).Effects of prohibitin in b-cells ... 5A). These data indicate thatexogenous PHB enters the cells.PHB is expressed in b-cells and increased byethanolTo determine the expression of PHB in b-cells we firstanalyzed the PHB protein...
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Tài liệu Báo cáo khoa học: "Japanese Dependency Parsing Using Co-occurrence Information and a Combination of Case Elements" pdf
... trainingdata)1.RerankingCandidate 1Candidate 2Candidate 3Candidate 4: Case element: VerbCandidateCandidateFigure 2: Selection of possible parses for rerankingMany methods for reranking the parsing of ... that simply adding it as a feature doesnot improve the accuracy.5.5.3 Changing the amount of training dataChanging the size of the training data set, weinvestigated whether the degree of ... training corpus by calculating the weightsfrom the frequencies of the features in the trainingdata.3 Japanese dependency analysis takingaccount of co-occurrence information and a combination...
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Báo cáo Y học: Conformationally constrained human calcitonin (hCt) analogues reveal a critical role of sequence 17–21 for the oligomerization state and bioactivity of hCt ppt
... demonstrate acrucial role of the conformational and topological features of the r esidues in sequence 17–21 and in particular of residues18 and 19 for human Ct receptor binding and in vivobioactivity ... would offer direct informationabout the role of the topographical features of the sidechains of the residues i n region 17–21 for receptor binding and in vivo bioactivity.Residues Lys18 and ... 18 and 19 of hCt and theirchirality are strongly associated with receptor binding affinity (c) inversement of the chirality of residues 18 and 19 strongly reduces the binding affinity of hCt and...
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