Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

... (labelled +2). (B) Cut-away view of L3 binding in the active site pocket showing the Phe-Phe clamp and the F229A mutation. Carbohydrate binding subsites are labelled )1, +1 and +2. Glycosidic bond cleavage occurs ... how the substrate is initially recog- nized by the particular enzyme and then drawn into the active site. Clearly, a detailed understanding...

Ngày tải lên: 15/03/2014, 23:20

... molecule binds at three subsites in the polysaccharide binding site of the TSbH domain of HylP2. The positions of ligands are indicated at the substrate binding groove. (B) The view from the top ... molecules, indicating the strong nature of the binding of ascorbic acid to proteins. Although ascorbic acid is a small molecule, it blocks the most attrac...

Ngày tải lên: 23/03/2014, 04:21

... DNA- binding properties of murine Six3, in vitro binding site selection demonstrated high-affinity binding to sequences containing the TAAT core motif, and clus- ters of these tetranucleotide sites ... binding mode of the HD and in uence the nucleotide contacts made by the recognition helix [6]. Notably, these different binding modes seemed to depend on the...

Ngày tải lên: 29/03/2014, 08:20

... depicts the binding isotherm; open circles represent the integrated binding heat of the data in the top panel, and the full line is the fit of a one -site binding model to the integrated binding data. ... 2011) doi:10.1111/j.1742-4658.2011.08043.x Starch -binding domains are noncatalytic carbohydrate- binding modules that mediate binding to granular starch....

Ngày tải lên: 06/03/2014, 00:21

... sequence lacking the PIN domain was obtained in two steps. First, the fragment of 423 bp situated in front of the PIN domain was amplified by PCR using forward primer 5¢- CGGGATCCCGGAGTCTGAGCTATGAGTG-3¢ ... RNase properties of MCPIP are exerted by the PIN domain, as shown by experiments with mutated MCPIP (either lack- ing the PIN domain, or with two conservative amino acid...

Ngày tải lên: 18/02/2014, 14:20

... amber. The classification based on sequence separation helps in assessing the in uence of SHBs at the local level or at the level of sequentially separated spatial interactions in the protein structure. ... [33,34]. The role of SSHBs in protein–ligand complexes has been studied and the donor and acceptor furcation has been analysed in detail [10], from t...

Ngày tải lên: 07/03/2014, 17:20

... evidence indicating that the phosphorylation of TSC2 at the S939 site comprises the primary recognition motif of 14-3-3 protein binding. Having established the importance of the S939 site in TSC2 ... collected at 10, 30 and 60 min after the initiation of IGF-1 stimulation. (A) Changes in phosphorylation states of Akt (T308 and S473 sites) and S6K1 (T...

Ngày tải lên: 15/03/2014, 11:20

... 2B). Because oxidative stress can induce uncoupling pro- tein 2 (UCP2) expression at the expense of ATP synthe- sis [28], we then determined the level of UCP2 protein and ATP production in RINm5F cells. ... (mitochondria) stain- ing; (C) anti-His (exogenous PHB)-FITC stain- ing; (D) merge. The arrows indicate staining of His-tagged PHB in (C) or both PHB and Mitofluor in...

Ngày tải lên: 29/03/2014, 08:20

... training data) 1 . Reranking Candidate 1 Candidate 2 Candidate 3 Candidate 4 : Case element : Verb Candidate Candidate Figure 2: Selection of possible parses for reranking Many methods for reranking the parsing of ... that simply adding it as a feature does not improve the accuracy. 5.5.3 Changing the amount of training data Changing the size of the training data set, we inv...

Ngày tải lên: 20/02/2014, 12:20

... demonstrate a crucial role of the conformational and topological features of the r esidues in sequence 17–21 and in particular of residues 18 and 19 for human Ct receptor binding and in vivo bioactivity ... would offer direct information about the role of the topographical features of the side chains of the residues i n region 17–21 for receptor bi...

Ngày tải lên: 31/03/2014, 21:21