Báo cáo khoa học: 5-Bromodeoxyuridine induces transcription of repressed genes with disruption of nucleosome positioning pptx

... arrayed beads of nucleosomes. Nucleosomes restrict the acces- sibility of transcription factors to promoters and regu- latory sequences of genes. Thus, an alteration of nucleosome positioning is ... mechanism of 5-bromodeoxyuridine K. Miki et al. 4548 FEBS Journal 277 (2010) 4539–4548 ª 2010 The Authors Journal compilation ª 2010 FEBS 5-Bromodeoxyuridine induces transc...

Ngày tải lên: 15/03/2014, 23:20

... factors as the mutation of the CRE site within the COX-2 promoter or the expression of a dominant negative mutant of CREB reduces inducibility of the COX-2 promoter [9,12–14]. The transcription factors ... stimulation of human monocytic THP1 cells leads to an induction of ESE-1 mRNA expression within 1 h of exposure, reaching a peak at 4 h and leveling off after 24 h [12]. To...

Ngày tải lên: 19/02/2014, 17:20

... member of the cap’n’collar family of basic leucine transcription factors and a well-established regulator of HO-1 [31]. In view of the beneficial effects of upregulation of HO-1 in models of organ ... University of Edinburgh, UK Cadaveric kidney transplantation is associated with substantial free radical injury as a consequence of cold storage and reperfusion of the org...

Ngày tải lên: 07/03/2014, 12:20

... transfected with 0.5 lg of pFlag–STA- T5A_FL, pFlag–STAT5A_DE18, or vector. Simultaneously, 0.5 lg of a firefly luciferase reporter plasmid with the mouse b-casein promoter (pCasein-luc) and 0.25 lgofa Renilla ... Functional characterization of the intermediate isoform of the human prolactin receptor. J Biol Chem 274, 35461– 35468. 16 Fang F, Antico G, Zheng J & Clevenger CV (20...

Ngày tải lên: 23/03/2014, 04:20

... phosphorylated and total levels of AKT. (B) Reduced phosphorylation of AKT with 1 l M wortmannin after 1.5 h of treatment was confirmed by immunoblot. (C) Cells were pretreated with or without 1 lM wortmannin ... reintro- duction of Fas expression abolished Fas resistance. Notably, the reduced amount of Fas in the resistant clones was a result of impaired FAS transcription. R...

Ngày tải lên: 23/03/2014, 06:20

... 150 ng of expression vector (pPacSp1 or pPacUSp3) was mixed with 50 ng of pDNaseII()249/+72)-Luc, and the total amount of DNA adjusted to 200 ng with pPac0. The DNA was mixed with 0.6 lLofFuGENE6in100lL ... incubating 5 lg of nuclear extract and 600 fmol of 32 P-labeled double- stranded oligonucleotide, with or without competitor, for 30 min at room temperature in a final vol...

Ngày tải lên: 23/03/2014, 17:21

... a heterodimer of Ets-1 and Elk-1 to the probe, and the N-terminus of Ets-1 and the C-terminus of Elk-1 were involved in the formation of the heterodimer. Preincu- bation of nuclear proteins with antibodies ... promoter activity of the gene. Furthermore, with the help of binding assays and site-directed mutagenesis, we established that Ets-1 ⁄ Elk-1 play a key role in the reg...

Ngày tải lên: 29/03/2014, 09:20

... available online: Fig. S1. Comparison of open reading frames of PrP and the splice variant of PrP (PrPSV) within exon 2 of PRNP in T98G cells. Fig. S2. Immunoblot analysis of recombinant human PrP and ... Division of Microbiology, National Institute of Health Sciences, Tokyo, Japan 2 Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Tokyo,...

Ngày tải lên: 30/03/2014, 04:20

... diffraction. As shown with dotted lines, the chemical shifts of the sig- nals of Ala116 and Ala118 are not affected by addition of PS, indicating that conformations of Ala116 and Ala118 within the C-terminal ... these conformational changes of the PH domain and induces the formation of a structure similar to that of the PH domain bound to IP 3 . The proposed structures of...

Ngày tải lên: 30/03/2014, 10:20

... terminally ill: the ‘risk of unacceptable badness’ David Crippen Associate Professor, Director, Neurovascular ICU, Department of Critical Care Medicine, University of Pittsburgh Medical Center, ... maintaining some of the vital functions of the body, but the same technology does not necessarily allow us to heal underlying disease processes [2]. An unintended side effect of modern...

Ngày tải lên: 25/10/2012, 10:45