Báo cáo khoa học: Frontal afﬁnity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for afﬁnity to agalactosylated N-glycans potx

... 2010 FEBS Frontal afﬁnity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for afﬁnity to agalactosylated N-glycans Rikio Yabe, Hiroaki Tateno and Jun HirabayashiResearch ... with agalactosylated cells and internalization of an agalactosylated glycoprotein.Identiﬁcation of agalactosylated glycoproteinligands for DC-SIGN in human serumIn order to identify agalactosylated ... determinant for thebinding of these lectins to agalactosylated N-glycans. By contrast, none of the lectins showed signiﬁcant afﬁnity to highly branched agalactosylated N-glycans. All of the lectins...

Báo cáo khoa học: Redox reaction between amino-(3,4-dihydroxyphenyl)methyl phosphonic acid and dopaquinone is responsible for the apparent inhibitory effect on tyrosinase doc

... Spectroscopic and elect-rochemical analysis of the oxidation of a mixture of Dopa and the inhibitor demonstrated that the phosphonic com-pound reduced dopaquinone back to Dopa, thus diminish-ing and ... potent inhibitor of tyrosinase [13]. With a Ki of 50 and 97 lM for tyrosine and Dopa as substrates, respectively,it was comparable with mimosine and tropolone. Themechanism of action of this compound ... again to 0 °C and 0.05 mol of PCl3was added. The mixture was heated and reﬂuxed for 2 h to complete the reaction. Acetic acid was removed byrotary evaporation and the oily residue was reﬂuxed for 10...

Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt

... 33 kDa and 28 kDa,respectively, and two additional protein bands at apparentmasses of 42 and 190 kDa [9]. For sequence analysis, theBAP31 immunoprecipitation protocol was scaled up and the ... components of the cytoskeleton actomyosin complex. A speciﬁc inter-action of myosin with BAP31 is supported by the presencein the cytosolic coiled-coil region of BAP31 (from Leu122 to Ala236) of four ... coverage (33% for myosin and 69% for actin) that lead us to conclude that the SDS/PAGE bands analysed, to the extent of the availablesequence information, contained only the proteins of interest....

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... + empty vector, DAT ⁄ P25a and DAT ⁄ a-syn to obtain equal concentrations of vector DNA wereincubated in the absence and presence of 0.5 mM DA for 24 h and subjected to analysis of viability ... instability of DA at the neutral pH of the cytosol causes the forma-tion of toxic aminochromes, and these species are notformed when DA is protonated at the acidic pH of thestorage vesicles. Factors ... used for analysis of DA and serotonin uptake, respectively.3[H]-DA was used to determine Vmaxcurves for DAT and NET, as3[H]-DA can also be used by NET, and 3[H]-serotonin was used for determination...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... a pair of acidic lobes, one of which iscomposed of residues from the SET-I region and theother of residues from the SET-C and post-SETregions. Lysine 4 of histone H3 is inserted into a chan-nel, ... Taspase 1 enzymeinto MLL-N and MLL-C fragments, whichreassociate through FYRN and FYRC motifs to form a stable complex. This matureprotein then assembles with a number of proteins to form MLL1 complexes ... [24,25] and the C-terminal SET domain in the presence and absence of histone peptides and the cofactor product,S-adenosyl-homocysteine (AdoHcy) [26] (Fig. 1B).These structures provide clues as to...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... used inhibitor in these studies, and it is usu-ally considered to be a speciﬁc inhibitor of PDI activ-ity [6–8]. However, in vitro evidence for the action of bacitracin as an inhibitor of PDI is ... speciﬁcity of action of an inhibitor for a protein-folding catalyst cannot be determined in vivo.Furthermore, in vitro evidence for the speciﬁcity of bacitracin for PDI isscarce, and the mechanism of ... thio-b-d-galactoside. Lysis of bacteria was performedby freeze–thawing the samples twice. PDI, PDI a domain,BiP and DsbA were puriﬁed by immobilized metal afﬁnity chromatography and anion exchange chromatography, ...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... binding of Zn, leading to the for- mation of SDS-soluble monomeric zinc-bound Ab and soluble copper-bound GIF [38]. Therefore, the metalswap led to simultaneous modiﬁcation of the ﬁnalform of Ab(1–40) ... hallmarks of AD isthe formation of b-amyloid (Ab) plaques, composedprimarily of Ab(1–40) and Ab(1–42) peptides, whichassociate to form abnormal extracellular deposits of ﬁbrils and amorphous ... MT isoforms, GIF bindsboth monovalent and divalent metal ions with high afﬁnity. They have the ability to inhibit heavy metaltoxicity [i.e. Cd(II), Hg(II)] and regulate the transport and storage...

Tài liệu Báo cáo khoa học: Steﬁn A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc

... theexception of E95, a stretch of four occluding loop resi-dues from V112 to S115 in the ﬁrst molecule of cathepsin B; G75 and Q76 in the molecule A of steﬁnA; and M1 and E78 in the molecule B of steﬁn ... aver-age distances between CA atoms of the active site cys-teine and histidine residues in cathepsins B and H and the center of CA atoms of steﬁns in the structures of both complexes. The average ... weakening of the embedded occludingloop in the active site cleft is not mandatory for theformation of the crystals of the complex, even thoughit is associated in a drop of Kifrom 0.93 to 0.35...

Tài liệu Báo cáo khoa học: The miRNA-192 ⁄194 cluster regulates the Period gene family and the circadian clock doc

... role for a cluster of miRNAs in the control of core components of thecircadian clock. Using a forward genetic screen to dis-cover miRNAs with regulatory capacities towards the3¢UTRs of all ... levels and decreased CCG expression [7]. This mode of repressionleads to the cycling of BMAL1 mRNA levels in ananti-phase fashion to that of the CCGs. When theother negative regulators of BMAL1, ... negative regulators of CLOCK ⁄ BMAL1, the family of Period genes (Per1,Per2 and Per3), the Cryptochromes (Cry1 and Cry2) and Rev-Erb a [3,5,6]. Rev-Erba binds the BMAL1promoter directly to inhibit...

Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... engine (MALDI-TOF data).dScores obtained using MS-Fit (MALDI-TOF data). For this latter analysis the top nonhomologous protein shows a score of 94.8.Diversity of cellulosomes and their synergies ... composition of the fractions was found to differ, particularly F1 and F6 which corresponded to the extremities of the peak (Fig. 2). In each fraction,Cel48F and Cel9E were found to be abundant. ... anti-CelM and anti-CelG serum.C, control sample corresponding to the unfractionated mixture of cellulosomes; F1–F6, fractions separated by anion-exchange chromatography. Diversity of cellulosomes and...

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