Báo cáo khoa học: Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans potx

... 2010 FEBS Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans Rikio Yabe, Hiroaki Tateno and Jun Hirabayashi Research ... with agalactosylated cells and internalization of an agalactosylated glycoprotein. Identification of agalactosylated glycoprotein li...

Ngày tải lên: 15/03/2014, 23:20

... Spectroscopic and elect- rochemical analysis of the oxidation of a mixture of Dopa and the inhibitor demonstrated that the phosphonic com- pound reduced dopaquinone back to Dopa, thus diminish- ing and ... potent inhibitor of tyrosinase [13]. With a K i of 50 and 97 l M for tyrosine and Dopa as substrates, respectively, it was comparable with mimosine and tropolone...

Ngày tải lên: 31/03/2014, 09:20

... 33 kDa and 28 kDa, respectively, and two additional protein bands at apparent masses of 42 and 190 kDa [9]. For sequence analysis, the BAP31 immunoprecipitation protocol was scaled up and the ... components of the cytoskeleton actomyosin complex. A specific inter- action of myosin with BAP31 is supported by the presence in the cytosolic coiled-coil region of BAP31 (from Leu1...

Ngày tải lên: 20/02/2014, 23:20

... + empty vector, DAT ⁄ P25a and DAT ⁄ a-syn to obtain equal concentrations of vector DNA were incubated in the absence and presence of 0.5 m M DA for 24 h and subjected to analysis of viability ... instability of DA at the neutral pH of the cytosol causes the forma- tion of toxic aminochromes, and these species are not formed when DA is protonated at the acidic pH...

Ngày tải lên: 14/02/2014, 21:20

... a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues from the SET-C and post-SET regions. Lysine 4 of histone H3 is inserted into a chan- nel, ... Taspase 1 enzyme into MLL-N and MLL-C fragments, which reassociate through FYRN and FYRC motifs to form a stable complex. This mature protein then assembles with a number...

Ngày tải lên: 16/02/2014, 14:20

... used inhibitor in these studies, and it is usu- ally considered to be a specific inhibitor of PDI activ- ity [6–8]. However, in vitro evidence for the action of bacitracin as an inhibitor of PDI is ... specificity of action of an inhibitor for a protein-folding catalyst cannot be determined in vivo. Furthermore, in vitro evidence for the specificity of bacitracin for...

Ngày tải lên: 16/02/2014, 14:20

... binding of Zn, leading to the for- mation of SDS-soluble monomeric zinc-bound Ab and soluble copper-bound GIF [38]. Therefore, the metal swap led to simultaneous modification of the final form of Ab(1–40) ... hallmarks of AD is the formation of b-amyloid (Ab) plaques, composed primarily of Ab(1–40) and Ab(1–42) peptides, which associate to form abnormal extracellular d...

Ngày tải lên: 16/02/2014, 15:20

... the exception of E95, a stretch of four occluding loop resi- dues from V112 to S115 in the first molecule of cathepsin B; G75 and Q76 in the molecule A of stefin A; and M1 and E78 in the molecule B of stefin ... aver- age distances between CA atoms of the active site cys- teine and histidine residues in cathepsins B and H and the center of CA atoms of stefins in the...

Ngày tải lên: 18/02/2014, 04:20

... role for a cluster of miRNAs in the control of core components of the circadian clock. Using a forward genetic screen to dis- cover miRNAs with regulatory capacities towards the 3¢UTRs of all ... levels and decreased CCG expression [7]. This mode of repression leads to the cycling of BMAL1 mRNA levels in an anti-phase fashion to that of the CCGs. When the other negativ...

Ngày tải lên: 18/02/2014, 06:20

... engine (MALDI- TOF data). d Scores obtained using MS-Fit (MALDI-TOF data). For this latter analysis the top nonhomologous protein shows a score of 94.8. Diversity of cellulosomes and their synergies ... composition of the fractions was found to differ, particularly F1 and F6 which corresponded to the extremities of the peak (Fig. 2). In each fraction, Cel48F and Cel9E we...

Ngày tải lên: 18/02/2014, 08:20