Báo cáo khoa học: Misfolded endoplasmic reticulum retained subunits cause degradation of wild-type subunits of arylsulfatase A heteromers pot
Báo cáo khoa học: Misfolded endoplasmic reticulum retained subunits cause degradation of wild-type subunits of arylsulfatase A heteromers pot
... C-terminus of wtASA (wtASA-HA) or ASAs carry- ing various amino acid substitutions (D335V-ASA-HA, T274M-ASA- HA, P136L-ASA-HA, G86D-ASA-HA and D255H-ASA-HA). (A) BHK cells were transiently transfected ... yielded plasmids designated D335V-ASA-HA, T274M-ASA- HA, P136L-ASA-HA, G86D-ASA-HA and D255H- ASA-HA. All of the respective missense mutations A B C Fig. 2. Secretion and stability of...
Ngày tải lên: 15/03/2014, 23:20
Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... Venkataraman V, Sait HBR, Kasinathan C & Ramasubbu N (2008) Probing the role of aromatic residues at the secondary saccha- ride-binding sites of human salivary alpha-amylase in substrate ... water-unextractable arabinoxylan (WU-AX) (A) and oat spelt xylan (OSX) (B) and of A. niger xylanase mutants to water-unextractable arabinoxylan (C) and oat spelt xylan (D). S. Cuyvers et al. Se...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf
... CGA TGG ATA CAG A- 3¢; reverse, 5¢-AGG ACA GTA GAA TTA GGT CAC T-3¢). Knockdown of Hsp10 5a and Hsp105b The double-stranded RNA targeting Hsp105 (Dharmacon; 5¢-GCA AAU CAC UCA UGC AAA CUU-3¢) was ... Buchberger A & Bukau B (1997) Interaction of Hsp70 chaperones with substrates. Nat Struct Biol 4, 342–349. 7 Hatayama T, Yasuda K & Nishiyama E (1994) Charac- terization of high...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf
... described in Garg et al. [13], whereas the data of WhiB3 and WhiB4 were taken from Alam & Agrawal [14] and Alam et al. [15] respectively. Md. S. Alam et al. Molecular properties of M. tuberculosis ... proteins of Mycobacterium tuberculosis H37Rv Md. Suhail Alam, Saurabh K. Garg* and Pushpa Agrawal Institute of Microbial Technology, CSIR, Chandigarh, India Mycobacterium tuberculosi...
Ngày tải lên: 18/02/2014, 12:20
Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... and functional properties of RNase and participates in degradation of IL-1b mRNA Danuta Mizgalska 1 , Paulina We˛ grzyn 1 , Krzysztof Murzyn 2 , Aneta Kasza 1 , Aleksander Koj 1 , Jacek Jura 3 , Barbara ... 5¢-TCCAGGCTGCACTGCTCACTC-3¢ (reverse). Each sample was normalized to reference genes: elongation factor 2 (EF-2), 5¢-GACATCACCAAGGGTGTGCA-3¢ (forward) and 5¢-TTCAGCACACTGGCATAGAGGC-3...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf
... s-DAPK-1Dtail was almost as active as full- length DAPK-1 (Fig. 4C). These data indicate that the ‘tail’ of s-DAPK-1 has a negative regulatory function with regard to s-DAPK-1 activity, and that its ... glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA quantification in colon carcinoma and rectal carcinoma as compared to normal colonic tissue. Colon carcinoma cells, rectal carcinoma ce...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Oxidized elafin and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf
... obtain cDNAs encoding M25L–elafin and M63L–trap- pin. For this purpose, forward primers 5¢-CGACTCGA GAAAAGAGCGCAAGAGCCAGTCAA-3¢ and 5¢-CGAC TCGAGAAAAGAGCTGTCACGGGAGTTCCT-3¢ were used for amplification ... inhibitor) has anti-microbial activity against Gram-positive and Gram- negative respiratory pathogens. FEBS Lett 452, 309–313. 13 McMichael JW, Maxwell AI, Hayashi K, Taylor K, Wallace WA, Gov...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf
... that of sbwAFP, has a m uch tighter coil path. Another effect of the tighter coils is that TmAFP has o ne and a h alf extra coils along the T XT face (Fig. 8A) . This gives TmAFP one and a half additional ... essentially identical ice-binding face that is considerably better at Fig. 8. A comparison of s bwAFP and TmAFP structures. (A) A n overlap of smoothed Ca traces obtain...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: The lipid ⁄ protein interface as xenobiotic target site Kinetic analysis of tadpole narcosis ppt
... the mechanism-related model was, at least for m ¼ 40, of comparable quality. Overall, statistical quality was determined by the quality of the data sets used. Discussion Animal narcosis Narcosis is assessed ... Shirahama K, Kamaya H, Ueda I & Totoki T (1991) Effect of inhala- tion anesthetics on swimming activity of Artermia salina. J Anesth 5, 287–293. 39 Horvath LI, Knowles PF,...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo khoa học: FGF-2, IL-1b and TGF-b regulate fibroblast expression of S100A8 doc
... Tedla, Z. Yang, W. X. Yan and H. Cai for technical help ⁄ advice. Funding from the National Health and Medical Research Council of Australia is acknowledged; FR held an Australian Postgraduate Award. References 1 ... and intranuclear S100C of normal human fibroblasts. J Cell Biol 149, 1193–1206. 11 Sakaguchi M, Tsuji T, Inoue Y, Miyazaki M, Namba M, Yamada H & Tanaka T (2001) Loss of...
Ngày tải lên: 19/02/2014, 18:20