Báo cáo khoa học: Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei pptx
... Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei Cornelia Welte, Christian Kra ¨ tzer and Uwe Deppenmeier Institute of Microbiology and Biotechnology, ... direct involvement of Ech hydrogenase in energy conservation: (a) ATP synthesis was observed in the Ms. mazei vesicular system that was dependent on the oxidation of Fd r...
Ngày tải lên: 15/03/2014, 23:20
... a notable 30- to 40-fold increase in cGMP levels during a 30-min incubation (Fig. 2); indeed a doubling of cGMP levels could be observed within 2 min of application of hormone (results not shown). ... YO. To investigate the initial stages of signal transduction, i.e. receptor binding, we determined receptor number (B max ) and affinity (K d ) of binding sites in membrane preparat...
Ngày tải lên: 21/02/2014, 00:20
... result of an imbalance between energy intake and expenditure. To state simply, chronic reduc- tion of energy expenditure versus intake causes an increased storage of the excess energy in the form of intracellular ... escalating prevalence of obesity is one of the most pressing health concerns of the modern era, yet existing medicines to combat this global pandemic are di...
Ngày tải lên: 06/03/2014, 01:20
Báo cáo khoa học: Exosites mediate the anti-inflammatory effects of a multifunctional serpin from the saliva of the tick Ixodes ricinus potx
... pain (stimulating scratch- ing) and hemostasis (repairing the wound and involv- ing coagulation), as well as innate, adaptive immune Keywords in ammatory; receptor binding domain; sepsis; serpin; ... 3235 reactions. In response, ticks have evolved a battery of countermeasures [3–7], mainly involving salivary pro- teins, several of which are induced during the feeding process and inject...
Ngày tải lên: 07/03/2014, 01:20
Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
... autoradiograms (Figs 2 and 3). In addition, an increased number of eosinophils were observed in lung interstitium of sensitized mice, con- firming the eosinophilic response following Der p 2 challenge ... followed in vivo both at the whole-body level and at the molecular level, through the application of a newly developed technique for specific labelling of recombinant proteins by...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: "PARSING VS. TEXT PROCESSING IN THE ANALYSIS OF DICTIONARY DEFINITIONS" pot
... success in parsing definitions with a minimum of incorrect or improbable parses, its success in identifying relational triples, and its speed. Input to the parser was a set of 8,832 definition ... most of the duplicate relational triples. Finding relational triples by text processing As the performance statistics above show, parsing is painfully slow. For the simple busines...
Ngày tải lên: 08/03/2014, 18:20
Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
... –Ca 2+ , 15 min in incubation medium; Ca 2+ , 15 min in incubation medium plus 3.5 l M Ca 2+ ;H⁄ R, 10 min hypoxia followed by 5 min reoxy- genation; CSA, 2 l M cyclosporin A. A volume of 2 mL of the incu- bation ... sug- gests that the mitochondrial injury is caused by open- ing of the mPTP. There is evidence that ADP inhibits opening of the mPTP by occupying binding sites locate...
Ngày tải lên: 16/03/2014, 22:20
Báo cáo khoa học: Novel suppression mechanism operating in early phase of adipogenesis by positive feedback loop for enhancement of cyclooxygenase-2 expression through prostaglandin F2a receptor mediated activation of MEK⁄ ERK-CREB cascade doc
... the molecular mechanism of this Fluprostenol-mediated increase in PGF 2a produc- tion in adipocytes, at first we investigated the expres- sion of genes involved in the biosynthesis of PGF 2a and ... MEK inhibi- tor, PD98059; a PLC inhibitor, U73122; a PKC inhibitor, bisindolylmaleimide I; and a general COX inhibitor, indomethacin, or a selective COX-2 inhibi- tor, NS-398, in the...
Ngày tải lên: 28/03/2014, 22:20
Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... curves. (C) Bars indicate the effects of preincubation on initial rates (5 min) of thrombin generation (n = 3–5). (D) Human platelets in fibrin-depleted human plasma were preincubated with inhibitors ... examined using two structurally distinct inhibitors, wortmannin and LY294002 [21]. In the presence of ADP ⁄ P2Y 12 activity, wortmannin or LY294002 suppressed the thrombin-induced Ca...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... mechanism of GAPDH could be essential for the binding of CP12. The presence of this region at the interface of GAPDH and CP12 could also explain the kinetic changes o bserved for the binding of ... made up of two f unctional domains, one corresponding to the cofactor-binding domain, or Rossman fold (residues 1–147 and 313–334 in spinach GAPDH (accession code in PDB:1JN0), the...
Ngày tải lên: 19/02/2014, 16:20