Báo cáo khoa học: Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei pptx
Báo cáo khoa học: Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei pptx
... Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei Cornelia Welte, Christian Kra¨tzer and Uwe DeppenmeierInstitute of Microbiology and Biotechnology, ... direct involvement of Ech hydrogenase in energy conservation: (a) ATP synthesiswas observed in the Ms. mazei vesicular system thatwas dependent on the oxidation of Fdred(catalysed by Ech hydrogenase) ; ... clearly exclude the involvement of Na+ in energy conservation by Ech hydrogenase. Instead,the data strongly support the model of proton translo-cation by Ech hydrogenase, leading to a direct contri-bution...
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Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt
... a notable 30- to 40-fold increase in cGMP levelsduring a 30-min incubation (Fig. 2); indeed a doubling of cGMP levels could be observed within 2 min of application of hormone (results not shown). ... YO.To investigate the initial stages of signal transduction, i.e.receptor binding, we determined receptor number (Bmax)and affinity (Kd) of binding sites in membrane preparations of YO obtained ... Neuropeptide-induced inhibition of steroidogenesis in crab moulting glands: involvement of cGMP-dependent proteinkinase. Gen. Comp. Endocrinol. 104, 41–51.20. Sedlmeier, D. & Seinche, A. (1998)...
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Báo cáo khoa học: Adipocyte hyperplasia and RMI1 in the treatment of obesity doc
... result of an imbalance between energy intake and expenditure. To state simply, chronic reduc-tion of energy expenditure versus intake causes anincreased storage of the excess energy in the form of intracellular ... escalating prevalence of obesity is one of the most pressing healthconcerns of the modern era, yet existing medicines to combat this globalpandemic are disappointingly limited in terms of safety ... suggestingthat these cyclin-dependent kinase inhibitors areimportant in the regulation of adipocyte number.The S-phase kinase-associated protein (Skp)1–Cullin-F-box protein (SCF) ubiquitin ligase...
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Báo cáo khoa học: Exosites mediate the anti-inflammatory effects of a multifunctional serpin from the saliva of the tick Ixodes ricinus potx
... pain (stimulating scratch-ing) and hemostasis (repairing the wound and involv-ing coagulation), as well as innate, adaptive immuneKeywords in ammatory; receptor binding domain;sepsis; serpin; ... 3235reactions. In response, ticks have evolved a battery of countermeasures [3–7], mainly involving salivary pro-teins, several of which are induced during the feedingprocess and injected at the site of ... goodinteracting domain because of a bad Berzofsky score.The properties of the four remaining selected peptidesare summarized in Table 2. The analysis indicatedthat: (a) domains 62–67 (overlapping...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
... autoradiograms (Figs 2 and 3). In addition, an increased number of eosinophils wereobserved in lung interstitium of sensitized mice, con-firming the eosinophilic response following Der p 2challenge ... followed in vivo both at the whole-body leveland at the molecular level, through the application of a newly developed technique for specific labelling of recombinant proteins by means of incorporating ... aradioactive selenocysteine residue in a C-terminalSel-tag [18].Der p 2 carrying the Sel-tag had an intact coresequence and maintained allergen-specific IgE-bindingepitopes and the use of a Sel-tag enabled...
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Báo cáo khoa học: "PARSING VS. TEXT PROCESSING IN THE ANALYSIS OF DICTIONARY DEFINITIONS" pot
... success in parsing definitions with a minimum of incorrect or improbable parses, its success in identifying relational triples, and its speed. Input to the parser was a set of 8,832 definition ... most of the duplicate relational triples. Finding relational triples by text processing As the performance statistics above show, parsing is painfully slow. For the simple business of finding ... definition grammar. Tagging and head finding were performed on a sample of 2157 noun definition texts, covering the nouns from a through anode. 170 were flagged as ambiguous; of the remaining...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
... –Ca2+,15 min in incubation medium; Ca2+, 15 min in incubation mediumplus 3.5 lM Ca2+;H⁄ R, 10 min hypoxia followed by 5 min reoxy-genation; CSA, 2 lM cyclosporin A. A volume of 2 mL of the incu-bation ... sug-gests that the mitochondrial injury is caused by open-ing of the mPTP. There is evidence that ADP inhibitsopening of the mPTP by occupying binding siteslocated in the inner and outer mitochondrial ... be involved in the patho-genesis of neurodegenerative processes such as tissueinfarction resulting from transient ischemia in stroke[1,2]. Investigations of in vivo ischemia showed thatbrains...
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Báo cáo khoa học: Novel suppression mechanism operating in early phase of adipogenesis by positive feedback loop for enhancement of cyclooxygenase-2 expression through prostaglandin F2a receptor mediated activation of MEK⁄ ERK-CREB cascade doc
... the molecular mechanism of this Fluprostenol-mediated increase in PGF2aproduc-tion in adipocytes, at first we investigated the expres-sion of genes involved in the biosynthesis of PGF2aand ... MEK inhibi-tor, PD98059; a PLC inhibitor, U73122; a PKCinhibitor, bisindolylmaleimide I; and a general COXinhibitor, indomethacin, or a selective COX-2 inhibi-tor, NS-398, in the presence of ... Involvement of MEK signaling in the enhancement of COX-2 expression. (A)Inhibition of Fluprostenol-activated COX-2expression by kinase and COX inhibitors.3T3-L1 cells were cultured in the presenceof...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... curves. (C) Bars indicatethe effects of preincubation on initial rates (5 min) of thrombin generation (n = 3–5). (D) Human platelets in fibrin-depleted human plasmawere preincubated with inhibitors ... examinedusing two structurally distinct inhibitors, wortmanninand LY294002 [21]. In the presence of ADP ⁄ P2Y12activity, wortmannin or LY294002 suppressed thethrombin-induced Ca2+integral ... (1993) ADP- andthapsigargin-evoked Ca2+entry and protein-tyrosinephosphorylation are inhibited by the tyrosine kinaseinhibitors genistein and methyl-2,5-dihydroxycinnamate in fura-2-loaded human...
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... mechanism of GAPDH could be essential for the binding of CP12. Thepresence of this region at the interface of GAPDH andCP12 could also explain the kinetic changes o bserved for thebinding of ... made up of two f unctional domains,one corresponding to the cofactor-binding domain, orRossman fold (residues 1–147 and 313–334 in spinachGAPDH (accession code in PDB:1JN0), the other being thecatalytic ... PRK binding.Keywords: phosphoribulokinase; glyceraldehyde-3-phos-phate dehydrogenase; CP12; site-directed mutagenesis;protein–protein i nteractions.Several lines of evidence point to the involvement...
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