Báo cáo khoa học: Kinetic and crystallographic analyses of t

Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf

... 2010 The Authors Journal compilation ª 2010 FEBS 2695 Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active ... 2010) doi:10.1111/j.1742-4658.2010.07685.x The hyperthermostable chitinase from the hyperthermophilic archaeon Pyrococcus furiosus...

Ngày tải lên : 15/03/2014, 11:20

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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... sulfonamide [R–S(O 2 )–NH– R¢] [23], sulfamate [R–O–S(O 2 )–NH–R¢] [24], sulfamide [R–NH–S(O 2 )–NH–R¢] [25,26], and N -acylsulfamate [R–O–S(O 2 )–NH–C(O)–R¢] [27]. Of these functional groups, only the ... [46]. The similarity in the K m values is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initial binding of both substrate...

Ngày tải lên : 14/02/2014, 22:20

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Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... sequence and the mass of the fragments, and from the kinetics of the process (Figs S7–S9 and Table S5) we concluded that the main sites of proteolytic cleav- age are at the C-terminus of the predicted ... minor, implying rotations of domains of just a few degrees (e.g. 7° interdomain rotations upon tRNA binding to the Tt-GluRS and local limited rearrangem...

Ngày tải lên : 07/03/2014, 03:20

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Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt

... tested N-heterocycles. In order to obtain more information about the binding properties of the active site of P450 mor and the permitted access of molecules to it, the binding of different azole compounds to the ... conditions. However, from the slope of the initial linear range of the kinetic plot, the constants V max ⁄ K m of 1.1 electronsÆmin )...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt

... concentrations ( 0–4 m M ). (B) A Lineweaver–Burk plot of the kinetic data. (C) A Hill plot of the kinetic data. Hill coeﬃcients are indicated as n H values. The n H value of 0.45 is given in the lower ... concentration of 1 m M into the reaction mixture. The results are shown in Table 1. The enzyme exhibited maximum activity in the presence of ATP, and...

Ngày tải lên : 08/03/2014, 22:20

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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... ade- nylosuccinate synthetase, one of whose substrates, IMP, binds to the GTP-binding site and inhibits the reaction [3 7–3 9]. The binding of pantoate to the ATP-binding site also reveals the origin of the negative cooperativity ... of pantoate bound in the ATP-binding pocket. Pantoate binding to the ATP-binding site induced large changes in structure, m...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... with ATMA and unaffected with o-NAC. The actual binding site of serotonin cannot be inferred from these results. Although serotonin can bind to the active site binding locus (W82), binding to PAS ... (a) the peripheral anionic site is involved in the non- linear activation and inhibition of the wild-type enzyme; and (b) in the presence of charged substrat...

Ngày tải lên : 18/02/2014, 17:20

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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and triﬂuoromethionine, a promising lead compound against amoebiasis ppt

... decrease in the k cat for Cys. This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination and c-elimination of the C–S bond, but not the b-elimination of the ... vector and the XbaI site in the EhMGL1 gene, the product of the nes- ted PCR was replaced with the corresponding region in pGEX–EhMGL1 [13] to pr...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx

... demonstrated the ‘sensitivity’ of the proximal region in determining the electronic and structural properties of the haem pocket. In the present study we attempt to obtain further insights into the extent ... an insight into the role of key catalytic residues [ 6–9 ]. A signiﬁcant outcome of many of these studies [6,1 0–1 2] has been the discovery of...

Ngày tải lên : 07/03/2014, 21:20

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Tài liệu Báo cáo khoa học: Applications and trends in systems biology in biochemistry docx

... acid, arginine catabolism to NO and polyamines NG, aorta endothelial cells Low afﬁnity transporter and arginase share the control of the ﬂuxes through the pathways involving arginine as a precurser of ... Eng 30, 20 2–2 16. 87 Choline, glycine betaine biosynthesis Nicotiana tabacum Import of choline into the chloroplast is a major but not the only important constraint on...

Ngày tải lên : 14/02/2014, 14:20

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