Báo cáo khoa học: Insulin like growth factor-1-induced phosphorylation and altered distribution of tuberous sclerosis complex (TSC)1⁄TSC2 in C2C12 myotubes pptx

... either activate or inhibit protein synthesis and cell growth [2]. The most well defined signaling mechanism regulating mTOR activity in skeletal muscle is the insulin like growth factor-1 (IGF-1) ⁄ insulin- dependent ... con- centration of wortmannin (10 lm) was required to inhibit insulin- induced activation of mTOR signaling in C2C12 myotubes (Fig. S1A) so we cannot r...

Ngày tải lên: 15/03/2014, 11:20

... is induced by postmitotic expression of insulin- like growth factor I. Mol Cell Biol 19, 3115– 3124. 24 James PL, Stewart CE & Rotwein P (1996) Insulin- like growth factor binding protein-5 ... Role of insulin- like growth factors in embryonic and postnatal growth. Cell 75, 73–82. 29 Louvi A, Accili D & Efstratiadis A (1997) A growth- promoting interaction o...

Ngày tải lên: 19/02/2014, 06:20

... among the resistance factor, kinetics of uptake, and kinetics of the P-glycoprotein-mediated efflux of doxorubicin, daun- orubicin, 8-(S)-fluoroidarubicin, and idarubicin in multi- drug-resistant K562 ... K562 cell line and its Pgp over-expressing subline (K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR), the 2008 cell line and its sublines ove...

Ngày tải lên: 18/02/2014, 13:20

... : 2000 in blocking solution for 30 min. Cells were rinsed in NaCl ⁄ P i several times and incubated in blocking serum containing anti-mouse IgG (Vectastain) for a fur- ther 30 min. Staining was ... composition, indicating that the peptide is of the correct sequence and free of contaminating pro- teins. Five cycles of N-terminal sequencing confirmed the expected residues includi...

Ngày tải lên: 18/02/2014, 13:20

... another is of fundamental importance for understanding telomere biology and the interactions of small molecules and proteins with telomeric DNA. The folding and unfolding of quadruplexes may be of pro- found ... Coupling of local folding to site-specific binding of proteins to DNA. Science 263, 777–784. 50 Lane AN & Jenkins TC (2000) Thermodynamics of nucleic acids and...

Ngày tải lên: 06/03/2014, 09:22

... [20]. In addition to the model of the ligand-free protein, the authors docked several GCPII inhibitors into the GCPII-binding pocket and proposed/ analyzed the amino acid residues involved in the ... the ligand– protein interactions. All of the residues identified are situated within the segment spanning Arg212 to Arg538, i.e. the putative catalytic domain (domain E) and the D doma...

Ngày tải lên: 07/03/2014, 15:20

... in ammation induces a state of ‘leptin resistance’ in obese animals and humans resulting in development of hyperleptinemia. Similarly, hyperleptinemia is a consequence of the development of insulin resistance in ... ANGPTL2 and ANGPTL6 ⁄ AGF is induced in obese conditions. ANGPTL2 induces chronic adipose tissue in ammation and systemic insulin resistance through th...

Ngày tải lên: 14/02/2014, 22:20

... by an N-terminal C2 domain responsible for guiding intracellular localization to internal membranes, four WW domains involved in substrate recognition and a C-terminal catalytic domain [1]. Itch ... produced (Fig. 1D, lane 2). In the presence of lactacystin, a sig- nificant increase in the amount of tBid–GFP present in the extract, both in control conditions and in the pres...

Ngày tải lên: 16/02/2014, 09:20

... proteinase K. DPP IV levels following stimulation with insulin and bpV(phen) In order to examine changes in DPP IV levels follow- ing insulin stimulation, rats were injected with the PTP inhibitor ... dependent of insulin with maximal association coincident with maximal tyrosine phosphorylation of DPP IV in vivo (Fig. 2). Insulin- dependent DPP IV phosphorylation is als...

Ngày tải lên: 19/02/2014, 07:20

... C-terminal cytosolic domain of SR-BI which is a critical domain involved in selective CE-uptake [29,53] is lacking in SR-BII and the lack of SR-BII protein in all three choriocarcinoma cell lines investigated ... choriocarcinoma cells (24 h in DMEM containing 10% FBS, 1% glutamine and 1% penicillin/streptomycin) and CHO cells (HAM’s F12 medium containing 5% (v/v) FBS, 2 m M...

Ngày tải lên: 20/02/2014, 23:20