Báo cáo khoa học: Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism doc

... al. Photoregulation of DNA transcription FEBS Journal 277 (2010) 1551–1561 ª 2010 The Authors Journal compilation ª 2010 FEBS 1561 Photoregulation of DNA transcription by using photoresponsive T7 ... [ 32 P]ATP[aP]) and the photoregulatory efficiency of transcription (a) was Fig. 1. Sequences of photoresponsive T7 promoters and the structures of Azo a...

Ngày tải lên: 15/03/2014, 10:20

... sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence path of order pÕ equal those of the arithmetic product of its Ó ... absolute magnitude of a combined autoinfluence path is the product of all the rates divided by the product of all the concentrations of its species. Therewith the c...

Ngày tải lên: 19/02/2014, 16:20

... structure of the reverse-joined ribozyme unit for twin ribozyme design consists of two domains joined by a single-stranded linker of eight adenosine residues and binds the 3¢-terminal part of its substrate ... construction of twin ribozymes. Even though HPTWRJ is not as efficient as its tandem- configured relative [16], its successful construction and the demonstration of...

Ngày tải lên: 20/02/2014, 01:20

... k þ off ð7Þ where k off(2) and k off(3) indicate values of k off occurring at K 2 < [ibuprofen] < K 3 , and at K 2 < K 3 < [ibuprofen], respectively, and k þ off is the k off value obtained ... Values of K 2 and K 3 were determined from data analysis, according to Eqn (7) [20]: k off ¼ðk offð2Þ Âð½ibuprofen=ðK 2 ½ibuprofenÞ þðk offð3Þ Âð½ibuprofen=ðK 3 ½ibuprofen...

Ngày tải lên: 06/03/2014, 01:20

... Call2 and CalliCu, Callinectes sapidus, 1, 2 and Cu isoforms (Uni- prot accession numbers Q548Y3, Q548Y2 and Q9U620, respectively); Scy1 and Scy2, Scylla serrata isoforms 1 and 2 (Uniprot P02805 and P02806); ... (Institute of Biochemistry of the University of Zurich, Switzerland) and also used as a template for the construction of regions coding for the deletion mutant C...

Ngày tải lên: 07/03/2014, 02:20

... levels of Neu5Ac [26] as well as glycine [20]. From the results (data not shown) of sugar (of LPS) and methylation (of LPS-OH) analysis, ESI-MS (of LPS-OH and OS), 1 H NMR (of LPS-OH) and ESI-MS n (of permethylated ... found) and Hep4-glycoforms (13 found), all of which can be represented by structures I and II (Hep3-glycoforms) and structure III (Hep4-glycoforms) in...

Ngày tải lên: 08/03/2014, 08:20

... effect of two variables, the reaction time and the presence of chloride ions, on the stoichiometry, stereochemistry and degree of folding of the Hg(II)-MT species formed by either the binding of Hg(II) ... understand the reaction pathways followed by Zn-MT and apo-MT species in the presence of Hg(II), the e ffect of each of the previous variables was analyzed separat...

Ngày tải lên: 16/03/2014, 18:20

... is a proof of concept and is limited to a small number of attributes and attribute-values. Part of our ongoing work involves investigation of scaling. For example, increasing the number of attribute- values ... dialogue using simple user goals. S=System, U=User. ‘—’ indicates the user barged in. fied in terms of the domain objects) and thus the role of the DM is one of...

Ngày tải lên: 08/03/2014, 21:20

... was scored by using its synonym amazed. Similarly, in-humane was scored using the reverse polarity of 26 its antonym humane, present in DAL. These scores are Z-Normalized using the mean and standard ... features including POS and word n-gram features (Section 5.3), and III. the combination of DAL scores and syntactic fea- tures to allow both n-gram analysis and polar...

Ngày tải lên: 31/03/2014, 20:20

... of repressed genes by disruption of nucleosome position- ing around their promoters. These data will facilitate the understanding of the role of BrdU and AT-tracts in the induction of particular genes. Results Construction ... necessary for recognition by the repressor [28] and thus its binding to the repressor may be disturbed by substitution of 5-bromouracil. In the...

Ngày tải lên: 15/03/2014, 23:20