Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt

... two a-helices on one side and three a-helices on the other side. Five equivalent subunits form a pentamer of the NH N H N N O O O OH OH OH OH O P O OH OH NH N H N N H O O O OH OH OH HO NH N H N N O O O OH OH OH HO O P ... to the absence of the aliphatic chain bearing the phosphate moiety. Whereas the inhibitor TS13 is composed of the purinetrione system and th...

Ngày tải lên: 07/03/2014, 11:20

... collection and refinement statistics. R symm = R h R i |I (h) ) I i (h) | ⁄ R h R i I i (h) , where I i (h) and I (h) are the ith and the mean measurements of the intensity of reflection h, respectively. ... [46]. The similarity in the K m values is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initial binding of...

Ngày tải lên: 14/02/2014, 22:20

... about three times higher than that of HYD Bp , and five times higher than that of HYD Pf (Table 1). As the activity of HYD Pf at the whole-cell level was lower than that of HYD Bp , even though it seems ... temperature of HYD Js of 50 °C for hydrolyzing d,l-p-HPH, which is the same as that for HYD Bp [19]. The optimal pH for the hydrolytic activity of HYD Js was 7.6,...

Ngày tải lên: 18/02/2014, 08:20

... and rainbow (right). The N-terminus and C-terminus are labeled in both monomers. On the left, the b-strands of the jellyroll domain are labeled, with the BIDG sheets in yellow and the CHEF sheet ... in the binding of the fiber protein in serotypes such as hAd2, which contain a long hypervariable region. The long hypervariable loop could directly interact with the...

Ngày tải lên: 19/02/2014, 06:20

... concentrations of the substrate, rEhPGDH showed an approximately eightfold higher affinity to NADH than NADPH, and specific activity was about threefoldhigherwithNADHthanwithNADPHinthe reverse direction ... space of the active site cleft and are thought to be responsible for the binding to 3-PGA, which is highly negatively charged with the phosphate and carboxyl groups [17]....

Ngày tải lên: 19/02/2014, 13:20

... FLAP for which the novel pattern is located in the third loop. The reason for the similarity and location of these pat- terns could be a result of short evolutionary time rather than gain of new ... identical to the fish sequences of other MAPEG families. The enzymatic properties of the pike MGST1-like enzyme were extensively characterised (Table 1) dem- onstrating th...

Ngày tải lên: 19/02/2014, 17:20

... position 4235 of the genomic clone as the translation start. The characterization of other clones revealed the presence of another cDNA (CaLOX2) sharing high identity, 91.9%, with the CaLOX1. The comparison of ... evaluate the expression of other genes of the lipoxygenase pathway involved in oxylipin biosynthesis (allene oxide synthase, hydroperoxide lyase), will...

Ngày tải lên: 21/02/2014, 00:20

... concentrations of the other substrates (129 ± 28 min )1 ), within the limits imposed by the high value of the K L-Glu that prevents an accurate estimate of this parameter, is now of the same order of magnitude ... sequence and the mass of the fragments, and from the kinetics of the process (Figs S7–S9 and Table S5) we concluded that the main sites of...

Ngày tải lên: 07/03/2014, 03:20

... sequence of the lac1 protein exhibits very low homology with sequences of other basidiomycete laccases (Fig. 7). Although the spectral characteristics of the lac1 protein suggest the absence of a ... Kinetics of PCR amplification with the electrophoretic image shown at the top. The cycle number (28·) that generates half maximal reaction was used to analyse the express...

Ngày tải lên: 07/03/2014, 15:20

... gel in the pH range 3–10 and the proteins were stained with Coomassie Brilliant Blue by the method of Neuhoff et al. [20]. The pI was determined by calculating the linear regression of the marker ... but the sensitivity of these sites to lamjapin was much lower than that of A4324 in the S/R domain. In order to confirm the multiple sites of depurination of lamjap...

Ngày tải lên: 08/03/2014, 10:20