Báo cáo khoa học: Thermosynechoccus elongatus DpsA binds Zn(II) at a unique three histidine-containing ferroxidase center and utilizes O2 as iron oxidant with very high efﬁciency, unlike the typical Dps proteins ppt

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): a unique metalloprotein ppt

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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modiﬁcations and in enhanced protective activity in sepsis doc

... binding as detected at A 405 nm. The results represent the average of at least three independent measurements, with the standard deviation indi-cated by error bars (gray bar, native Z2 antibody; ... by the Animal Care Commissionof the Institute of Microbiology, in accordance with National and European Regulations. The number of ani-mals used was kept at the minimum that still ensured statis-tical ... analysisStatistical analyses were performed using graphpad prism,version 4.00 (GraphPad Software, San Diego, CA, USA).Statistical analyses of the ELISA data and the areas under the curve of the immunoblot...

Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx

... intermediate. We also propose that the seven-fold higher efﬁciency of RNA cleavage by RNaseSa than by RNase Sa2 can be at least partly explainedby the Val43 (RNase Sa) to Arg45 (RNase Sa2)substitution. ... nucleotide.Discussion The goal of the present work was to better understand the catalytic mechanism of RNase Sa2 and to accountfor the differences in catalytic activity between RNasesSa2 and Sa. On the basis of the ... to the oxygen and facilitating O2 attack on the phosphorus atom. The cyclophosphate intermediate isformed, and the 5¢-strand of RNA is leaving from the active site. The ﬁgure was drawn with ISIS...

Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

... agreement with thoseagainst thermal denaturation.Stabilization of 4·-RNase HI with Arg97ﬁGly and Asp136ﬁHis mutationsTo examine whether the Arg97 ﬁ Gly and Asp136 ﬁHis mutations stabilize ... intoSo-RNase HI or 4·-RNase HI. The stabilities of the 4·-RNase HI, 5·-RNase HI and 6·-RNase HI proteins against thermal denatur-ation were analysed as described for R97G-RNase HI and D136H-RNase ... ﬁ Lys, Asp39 ﬁ Gly, Met76 ﬁ Val and Lys90 ﬁ Asn mutations; 5·-RNase HI, 4·-RNaseHI derivative with additional Arg97 ﬁ Gly mutation; 6·-RNase HI, 5·-RNase HI derivative with additional Asp136...

Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc

... Saitou1, Takehisa Atsumi1, Takashi Sato1, Ken Ando1, Shuntaro Hara2, KiyomiKikugawa1 and Makio Hayakawa11 School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan2 ... mutagenesis byPCR as follows. The pBluescript SK vector (Stratagene, LaJolla, CA, USA) encompassing an HIF- 1a cDNA in whichPro564 and Asn808 were replaced with alanines was used as a template. ... denatured at 90 °C, cooled for annealing, and used to knock downHIF- 1a. Similarly, the siRNA against HIF- 2a was preparedusing the sense (5¢-CAGCAUCUUUGAUAGCAGUdTdT-3¢) and antisense (5¢-ACUGCUAUCAAAGAUGCUGdTdT-3¢)...

Tài liệu Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage pdf

... the combinedeffects of ALLN and TRAIL in canine chondrocytes,western blot analysis was performed. It was shownthat cotreatment of cells with ALLN and TRAILincreased caspase-8 activation and ... demonstrated that ALLN pretreatment with TRAIL increased the activation of caspase-8under normoxic conditions, but that caspase-8activation was inhibited under hypoxic conditions. Inaddition, the ... inhibitionenhanced TRAIL-induced cell death via the caspasepathway, the key regulator of the TRAIL-induced celldeath pathway in canine chondrocytes. Furthermore,cotreatment of cells with both ALLN and...

Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... via two engineeredrestriction sites (NdeI and EcoRI) introduced by PCR with the primers 5¢-GCTATTGTGGTAGAATACATATGAGACAC-3¢, sense, and 5¢-GGAGTTGTAAAAATTCGAATTCTAAAGAGC-3¢, antisense (the ... culture and homogenate preparationSulfolobus solfataricus was isolated from an acidic hotspring in Agnano (Naples, Italy) and grown at 87 °Caspreviously reported [51]. For the homogenate preparation,10 ... centrifu-gation and lysed as described by Sambrook et al. [52]. The cell debris was removed by centrifugation at 20 000 gfor 60 min at 4 °C and the supernatant was used as a cell extract.Preparation...

Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf

... receptorprotein-tyrosine phosphatases (RPTPs) have two intra-cellular PTP domains. The membrane proximaldomain (D1) contains most catalytic activity, whereas the membrane distal domain (D2) has a regulatoryfunction ... to theirectodomains. To investigate the role of the cytoplasmic domain in dimer-ization we generated RPTPa ectodomain (EDa) ⁄ RPTP chimeras and foundthat – similarly to native RPTPa – oxidation ... oxidationinduced conformational changes in the cytoplasmicdomain of all four RPTPs tested, altering rotationalcoupling within RPTP dimers. These conformationalchanges may regulate the catalytic...

Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

... Colavita AR, FitzGerald GA & Violi F(1997) Platelet activation by superoxide anion and hydroxyl radicals intrinsically generated by platelets thathad undergone anoxia and then reoxygenated. ... cyto-plasm to the plasma membrane, where it associates with 5-LOX-activating protein (FLAP) and metaboli-zes arachidonic acid to release ROS [28,29]. The phos-phatidylinositol-3-kinase (PI3K) ⁄ Akt ... time-dependent.Administration of apocynin (NADPH oxidase inhibitor) attenuated the febrile response to the supernatants in rabbits and decreased the transloca-tion of NADPH oxidase p47phoxsubunit and NF-jB activity...

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... determined at about 10-fold lower protein concentration than the measurements of inactivation, show the sametrends with respect to the effects of NaCl, CaCl2 and EDTA as the inactivation constants. As ... plot of the data from (A) in the range 0–100 mM Ca2+. (C) Modiﬁed Scatchardplot of the data from (B). DA corresponds to the change in relative activity. The PLDa2 activity was measured against ... again, and the decrease was steeper with cal-cium acetate than with calcium chloride. Interestingly, the activity data in the range 0.01–100 mm CaCl2could be ﬁtted in similar way as the near-UV...

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