Báo cáo khoa học: Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein doc

... 5¢-ATC CGG GGT CTC CCA- TG GCA ATG AGG GCT GCG GGT G-3¢; HCV-1b Core+1/S sense, 5¢-ATC CGG GGT CTC CCATG GCA ATG AGG GCC TGG GGT G-3¢; HCV-1a Core+1/S antisense, 5¢-AT CCG GGT CTC GGTACC TTA TCA CGC ... reading frame protein; DLS, dynamic light scattering; HCC, hepatocellular carcinoma; HCV, hepatitis C virus; HSQC, heteronuclear single quantum coherence; IDP, intrinsically disordered...

Ngày tải lên: 15/03/2014, 09:20

... also converged to indicate an abundance of intrinsic disorder in SV-IV, but few amino acids in the C- terminal region. In particular, hydrophobic clusters, which are typical of secondary structure ... recognition accuracy can be obtained by an SVM that incorporates only amino acid composition, and very good recogni- tion accuracy was retained using reduced sets of amino aci...

Ngày tải lên: 23/03/2014, 07:20

... the expression of activating transcription factor 4 (ATF4) and of CCAAT⁄ enhancer-binding protein homologous protein (CHOP) proteins downstream of eIF2a were observed (Fig. 4A), indicating the initiation of ... of a variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proin- flammatory cytokines, inhibitio...

Ngày tải lên: 14/02/2014, 22:20

... was also accompanied by a decrease in the Trp fluorescence intensity. Plotting the percentage of initial fluorescence as a function of the lipid concentration (Fig. 2) enabled calculation of K d values. ... wavelength of 290 nm, at a slit width of 4 nm. The fluorescence spectra were instrument corrected for light scattering, by subtract- ing the corresponding spectra of...

Ngày tải lên: 07/03/2014, 17:20

... be binding sites for the Orc1 ⁄ Cdc6 proteins. The oriC1 origin is located upstream of the cdc6-1 gene, encoding the Sulfolobus ortholog of the Pyro- coccus Orc⁄ Cdc6 and Halobacterium Orc7 proteins. Moreover, ... exam- ple, the positioning of nucleosomes at an origin can be in uenced by the binding of ORC [29]. Additionally, the acetylation status of chromatin can...

Ngày tải lên: 07/03/2014, 21:20

... provides the residues for binding the coenzyme. The C- terminal domain has a lar- gely a-helical structure, and serves mainly in substrate binding. The active site is located in the interdomain cleft, ... and so the choice of the constant level of Fru was not critical for determination of D K m (1.23 ± 0.15). The KIE data in Table 4 are instrumen- tal in delinea...

Ngày tải lên: 14/03/2014, 23:20

... [24]. Interestingly, phos- phorylation of CYP1A1 ⁄ 1A2 occurred at 24 h after Sham GdCl 3 CLP GdCl 3 + CLP CYP1A1 β-actin β-actin β-actin β-actin CYP2B1 CYP2E1 CYP1A2 Fig. 4. Effects of KCs on ... metabolic activation of numerous procarcino- gens, including aflatoxin B1 [21]. In the present study, CYP1A1 and 1A2 activities were significantly decreased, with a concomitant decrease in...

Ngày tải lên: 14/02/2014, 18:20

... (2001) Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phos- phorylation of Cdc24p. J Biol Chem 276, ... is consistent with the genetic interactions reported previously. The dele- tion of either ERG4 or NCP1 in the cla4D background is lethal, indicating that CLA4 and these genes,...

Ngày tải lên: 18/02/2014, 13:20

... nuclear extracts from SP1 cells. The nucleo- tide sequences of putative CERK-PPRE, including the sense 5¢-CTCTCCAGGCCACAGGCCAGAGCGG-3¢ and anti-sense 5¢-GAGAGGTCCGGTGTCCGGTCTCGCC-3¢ sequences, ... to sphingosine and then recycled into intracellular Cer itself, and that accumu- lation of Cer contributes to the induction of apoptosis [20]. However, the signaling mechanism involved...

Ngày tải lên: 18/02/2014, 18:20

... tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans Parvinder ... activator of the mauF promoter were amplified from genomic DNA using forward (5¢ -TTGGT AAGCTTGGGCATTTCTGAT CGGGTCGC-3¢) and reverse (5¢-AAAC CATATGACGCC TCCTCTCGCT-3¢)...

Ngày tải lên: 19/02/2014, 07:20