Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf

... and C-terminal domain, including the highly conserved GSH -binding site (G -site) and the more divergent cosubstrate -binding domain or hydrophobic binding site (H -site) [7,18]. The G -site includes both a-helices and ... Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases...

Ngày tải lên: 15/03/2014, 09:20

... GATA-transcription factor binding site in the c-T2 promoter, although other processes might also be involved. In multicellular organisms, GATA -binding factors play critical roles in development, including cell-fate ... participates in the assembly of the mitotic spindle of E. focardii and that both isotypes are involved in the nucleation of other microtubule st...

Ngày tải lên: 07/03/2014, 04:20

... homologues in the context of DNA binding and chromatin regulation. These homologues include members of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated CpG islands ... inhi- bitor of growth protein 3 (ING3) PHD, which is another domain involved in chromatin binding and clearly more divergent (e-value 0.019) from DIDO and the other sequenc...

Ngày tải lên: 07/03/2014, 21:20

... fitting procedure to determine total and nonspecific binding at the same time. In saturation binding experiments the experimentally determined nonspecific binding was used to estimate nonspecific binding ... [28]. The N-terminal sequence of the H1 receptor indeed contains two N-linked glycosylation consensus sites, but in prelim- inary studies no binding to concanavalin...

Ngày tải lên: 30/03/2014, 14:20

... promoting an increase in proopiomelanocortin and cocaine and amphetamine regulated transcript. Taken together, these findings provide a compelling argument for the role for malo- nyl-CoA in regulating ... needs to be explored further. Other areas of the brain have also been shown to regulate feeding via AMPK [35,36]. Of interest is the extensive use of fructose as a sweetener...

Ngày tải lên: 14/02/2014, 22:20

... following exposure to calcium iono- phore, indicating that they were correctly targeted into secretory granules even in the absence of serglycin. In the absence of serglycin, the fates of the serglycin-dependent ... on serglycin. Together, these findings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by missorting in...

Ngày tải lên: 07/03/2014, 11:20

... increase in the amount of ROS [21]; (b) loss of cytochrome c impairs the flow of electrons in the respiratory chain inducing over- reduction of the complexes, favouring the generation of ROS [16,17,22]; ... element binding protein, iso- citrate dehydrogenase is an RNA -binding protein, while kgd2p is a mitochondrial DNA binding protein [81–84]. Mitochondria from d...

Ngày tải lên: 07/03/2014, 12:20

... concentrations. The d 2 -crystallin in lens was recruited during evolution from argininosuccinate lyase, an enzyme involved in arginine biosynthesis derived from the urea cycle. These two proteins shared ... stability for crystallins is essential in maintaining lens transparency [4]. Determining the mechanism of folding and assembly of these proteins is important for underst...

Ngày tải lên: 08/03/2014, 08:20

... chain is brefeldin A-insensitive and is inhibited by the proteasome inhibitor clasto-lactacystin b-lactone, resulting in the accumulation of ricin A chains. These stabilized ricin A chains are partly deglycosylated ... Three high-lysine mutations control the level of ATP -binding HSP70-like proteins in the maize endosperm. Plant Cell 3, 507–515. 69 Zhang F & Boston RS (19...

Ngày tải lên: 16/03/2014, 11:20

... detec- ted in the perinuclear region of the CHO cells with a punctate distribution in the cytoplasm of the cell (Fig. 2A). The perinuclear staining of the wt ZIP1-Myc protein was strikingly coincident ... the di-leucine signal of ZIP1 and substitutions of the di-alanine residues for the di-leucine residues in the ZIP1 sorting signal inhibited the inte...

Ngày tải lên: 16/03/2014, 11:20