Báo cáo khoa học: Membrane anchoring of diacylglycerol lactones substituted with rigid hydrophobic acyl domains correlates with biological activities ppt

... constrained analogues of diacylglycerol (DAG). 27. Modulation of membrane translocation of protein kinase C (PKC) isozymes a and d by diacylglycerol Membrane interactions of diacylglycerol derivatives ... al. Membrane interactions of diacylglycerol derivatives FEBS Journal 277 (2010) 233–243 ª 2009 The Authors Journal compilation ª 2009 FEBS 243 Membrane anchoring...

Ngày tải lên: 15/03/2014, 09:20

... formation of a distinct peptide of 27 kDa after trypsin treatment of cells indicates the existence of one large extension protruding at the external side of the plasma membrane. From the size of the ... trans- membrane domains of proteins [17,33,34]. [ 125 I]TID parti- tions efficiently into membrane lipid bilayers and photolabeling of proteins with [ 125 I]TID occur p...

Ngày tải lên: 21/02/2014, 00:20

... that parts of the hepatic activities of diacylglycerol acyltransferase and acyl choles- terol acyltransferase are expressed in the lumen of the endoplasmic reticulum (ER). However the ER membrane ... mitochondrial one. Enzymes within the lumen of the endoplasmic reticulum (ER) also require fatty acyl- CoA thioesters as substrate. A latent form of diacylglycerol acyltransf...

Ngày tải lên: 16/03/2014, 16:20

... immuno- blotting of the pelleted membrane fraction with anti-FtsY serum. Despite the predicted absence of any membrane- spanning domains, the inability of either urea or sodium carbonate to solubilize membrane- bound ... presence of significant levels of FtsY associated with the IMVs (Fig. 6C). Finally, the presence of 5 m M GTP, GDP or GTPcS had no discernable effect on...

Ngày tải lên: 16/03/2014, 16:20

... was not stimulated with EGF. That EGFr has a homogeneous distribution (a scarce association with microdomains) in membranes from CHO-K1 cells is consistent with the behaviour of the EGFr in both ... plane was mainly adjusted through the top of the cell, allowing the visualization of larger plasma membrane areas and the identification of small membrane domains (Fig. 5E–H). T...

Ngày tải lên: 16/03/2014, 16:20

... form capable of integrating into the membrane without ATP, probably because of conformational changes induced by the loss of its cofactor. The overall findings suggest that a pathway exists for the membrane ... most of the required functions, such as binding of Tat substrates, recognition of the folded state, formation of a translocation pore, usage of the DpH for transloca...

Ngày tải lên: 23/03/2014, 21:20

... available: Fig. S1. Stereoview of the catalytic cleft of Hyb-24DN. Fig. S2. Stereoviews of surface structure of Ald-bound and unbound Hyb-24DNY. Fig. S3. Stereoview of the catalytic cleft of Hyb- 24DNY and ... Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Japan 2 Department of Life Science, Graduate School of Life Sci...

Ngày tải lên: 07/03/2014, 00:20

... acetonitrile with a flow rate of 1 mLÆmin )1 . The amounts of CD 6 to CD 24 were quantified by comparison with standard curves of authentic CD 6 to CD 24 samples. Derivatization of the CGTases by acylation ... respectively. With a ratio of 1 : 3, the remaining activity of both enzymes was not significantly different from a ratio of 1 : 5, but the derivatization degree was sig...

Ngày tải lên: 07/03/2014, 10:20

... onstructed mutant protein of P450nor of Fusarium oxysporum in which thenegativechargeofeachoftheseresidueswascancelled by replacing it with a neutral residue. Some of the mutant proteins were ... NADPH of 12.7 s )1 and 0.64 m M , Fig. 3. Spectral changes during reduction with NADPH of the Fe 3+ – NO complex of the wild-type (A) and D88A mutant (B) of P450nor. Each spectrum w...

Ngày tải lên: 07/03/2014, 15:20

... Properties of the enzymatic steps/enzymes of the a-oxidation pathway. The table gives an overview of the present knowledge of some of the properties of the enzymes involved in the initial degradation of ... a-Oxidation of 3-methyl -substituted fatty acids in rat liver. Production of formic acid instead of CO 2 , cofactor requirements, subcellular localization and formatio...

Ngày tải lên: 08/03/2014, 02:20