Báo cáo khoa học: Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting pdf

... -9,-11 and -19) and ADAM12 cleave IGF-bindingFig. 1. Pivotal roles of MMPs in cancer progression. Cancer progression involves different stages, including tumor growth and the multistepprocesses of ... [52,53]. Pharmacological targeting of matrix metalloproteinases On the basis of the pivotal roles that MMPs and ADAMs play in several steps of cancer progression, the pharmaceutical industry has invested ... ectodomain shedding and nonpro-teolytic functions, such as binding to adhesion mole-cules, integrins and interacting with phosphorylationsites for serine ⁄ threonine and tyrosine kinases,...

Báo cáo khoa học: Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus pptx

... exam-ine the roles of the conserved arginines, Arg322 (P2) and Arg323 (P1) of AAA-1, and Ar g747 (P1) of AAA- 2, and determined that the arginines of TClpB are notinvolved in nucleotide binding ... these three arginines in TClpB.By combining an arginine mutation with the WalkerAmutation in the other AAA+ module, we tested the roles of the arginines in ATP binding and hydrolysis in each module. ... arginines are not involved in nucleotide bindingTo examine the roles of conserved arginines, we gener-ated three mutants of TClpB: R322A [1R ⁄ A(P2)],R323A [1R ⁄ A(P1)], and R747A (2R⁄ A). In...

Báo cáo khoa học: Demethylation of radiolabelled dextromethorphan in rat microsomes and intact hepatocytes Kinetics and sensitivity to cytochrome P450 2D inhibitors pot

... hepatic protein binding of quinine and quinidine. Accumulation of quinine and quinidine (5 lM) in rat hepatocytes is expressed as the steady state ratio (C/M) betweencell associated and extracellular ... concentration.Determination of drug binding to hepatic proteinsFor the determination of the liver tissue binding of [3H]quinine and [3H]quinidine, rat liver was homogenized in 0.1Mpotassium ... most inhibitors,including quinine and quinidine, displayed 2–3-foldlower IC50 in hepatocytes than in microsomes. The cell-associated concentrations of quinine and quinidine werefound to be...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... domain at their C-terminus, and a lessconserved proline-rich and glutamine-rich transactiva-tion domain at their N-terminus [8–10]. Most isoformsalso have a PY-motif (XPPXY) in the N-terminal ... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen-tiation stages during chondrogenesis and the involvement of transcription factors(henatoxylin and eosin-stained section of ... REVIEW ARTICLE Roles of AP-2 transcription factors in the regulation of cartilage and skeletal developmentAnn-Kathrin Wenke and Anja K. BosserhoffInstitute of Pathology, University of Regensburg,...

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the ... would enhance or diminish the impact of HSF2 deﬁciency.Pending questions for the roles of HSF1 and HSF2 in oogenesis and in early embryosThe role of HSF2 in oogenesis and in pre-implantationdevelopment ... HSF4-bindingregions and of the transcriptional status of geneslocated around the HSF4-binding regions. This resultis thus suggestive of a structural effect of HSF4 onchromatin. In the absence of...

Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc

... within the L1loop with alanine, a short side chain amino acid, signiﬁcantly decreased theDNA-binding ability of human Rad51, without affecting the protein fold-ing or the salt-induced, DNA-independent ... signiﬁcantdefects in ssDNA binding and dsDNA bindingABFig. 3. The DNA binding activities of the Rad51 mutants. (A) The ssDNA binding experiments. The /X174 circular ssDNA (40 lM) was incu-bated ... changes of tryptophan probes inserted in theL1 and L2 loops of Rad51 upon poly(dT) binding. The ﬂuorescenceintensities of 1 lM modiﬁed Rad51, in which a tryptophan probewas inserted within the...

Báo cáo khoa học: Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions pptx

... application of SH2 domain binding peptides, which results in blocking of the binding of the SH2 domain to thesubstrate and thereby preventing interaction of the substrate with the kinase domain. For ... demonstrated that intra-domain interactions betweenSrc family kinases (SFKs), stabilized by binding of the phosphorylatedC-terminus to the SH2 domain and ⁄ or binding of the SH2 kinase linker tothe ... studies of inactive and active Src, the SH2 and SH3 domains are believed toform a ‘regulatory apparatus’. Binding of the phos-phorylated C-terminus to the SH2 domain and ⁄ orbinding of the SH2-kinase...

Báo cáo khoa học: Roles of 1-Cys peroxiredoxin in haem detoxiﬁcation in the human malaria parasite Plasmodium falciparum potx

... that only a trace of the pro-tein was left in the unbound fraction. Pre-incubation of the recombinant protein with FP abolished most of thisbinding, suggesting that binding of rPf1-Cys-Prx to ... the original activity (–). HGindicates inactivation reactions containing FP and GSH. HGP and HGaP indicate inactivation reactions containing HG + rPf1-Cys-Prx and HG + autoclaved rPf1-Cys-Prx, ... manner and reducing Fe-derived ROS, pro-tecting the proteins in the cytosol from ROS and, ﬁnally, interfering with formation of the membrane-associated FP. Further studies of such functions of...

Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf

... processing of one or several nuclear proteins, whereas the other isindependent of the processing capacity of the protein-ase and involves the HPX domain. A DNA-bindingdomain was found in the ... disease: roles of matrix metalloproteinases in cancer progression and their pharmacological targeting. FEBS J 278, 16–27.16 Tchougounova E, Lundequist A, Fajardo I,Winberg JO, Abrink M & ... illustrated in Fig. 2B (step 1).Interactions with small integrin-binding ligandN-linked glycoprotein (SIBLING)Individual members of the SIBLING family are knownto bind strongly to both pro- and active...

Báo cáo khoa học: Roles of base excision repair subpathways in correcting oxidized abasic sites in DNA pptx

... physical and chemical agents, includingUV and c-irradiation, heterocyclic N-oxides of thetirapazamine family, organometallic oxidants and the anticancer antibiotics (such as neocarzinostatin) of the ... path-ways. In this model, the removal of 5¢-dRp, whichappears to be the late-limiting step in short-patch BER[64], may be critical in determining the mode of BER.DNA–protein crosslink formation in ... thereby preventing the formation of deleteriousDPC adducts in DNA.Concluding remarks In spite of numerous efforts in deﬁning the biological and biochemical mechanisms involved in BER, the cel-lular...

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