Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc

... formation and identify a region, located within the C-terminal domain of Ure2p that interacts with Ssa1p. Because the C-termi- nal domain of Ure2p is tightly involved in the assembly of the prion into ... His-Tagged Ssa1p (B). (A) A mixture of untreated Ure2p and Ssa1p (lane 1); Ure2p alone (lanes 2, 5, 8 and 11), Ssa1p alone (lanes 3, 6,...

Ngày tải lên: 15/03/2014, 00:20

... cell intoxication, diphtheria toxin is cleaved by furin into two fragments: the A chain corresponding to the catalytic domain (C domain) ; and the B chain corresponding to the translocation domain ... (N) state. Thus, the MS approach used in this work offers a valuable alternative. The data enabled us to identify the core of the pro- tein in the N- an...

Ngày tải lên: 16/02/2014, 09:20

... rates of respir- ation (A) , RCR (B) and the ratio of the rates of state 3 respiration before and after the addition of NADH and cytochrome c (C) are presented. The rate of state 3 respiration of ... mitochondrial damage. Our data demonstrate that hypoxia ⁄ reoxygenation and extramitochondrial Ca 2+ cause functional damage of isolated rat liver mitochon- dria. Es...

Ngày tải lên: 30/03/2014, 11:20

... an extra effector in which the activation domain of viral protein 16 was fused to the yeast GAL4 DNA binding domain (DBD) and then coexpressed with the AtERF4 effector was used to test the in ... AtERF family is further divided into various subgroups according to the homology of ERF domains [5,6]. An ERF domain consists of a three-stranded anti- parallel b-...

Ngày tải lên: 18/02/2014, 13:20

... Com- mittee of the University of Balamand and complied with the principles of laboratory animal care. Chemicals and reagents Muramyl peptides (MDP and MB) used in this work were kindly provided by ISTAC-SA ... immune and ⁄ or inflammatory responses [14,15]. Infected perito- neal macrophages of UCP2 ) ⁄ ) mice are resistant to infection by the intracellular parasit...

Ngày tải lên: 05/03/2014, 23:20

... extra- cellular ligands [4,5]. Besides the extracellular domain, the majority of the RPTPs present another interesting feature: tandem catalytic domains. The membrane- proximal catalytic domain (D1) and the ... January 2010) doi:10.1111/j.1742-4658.2010.07584.x Receptor protein-tyrosine phosphatase a (RPTPa) is a transmembrane pro- tein with tandem cytoplasmic phospha...

Ngày tải lên: 15/03/2014, 10:20

... s a ch a các mặt bích hở 80mm ÷ 90mm là thay đoạn ống bị hụt bằng đoạn ống dài hơn (khi nối ống có lót ống bên trong). Giàn giáo phục vụ s a ch a lắp dựng ở r a sàn thao tác. Theo đề xuất c a ... phải v a đảm bảo mức chuyển vị nằm trong phạm vi tính to n thiết kế đ a ra là 100mm, v a đảm bảo an to n tuyệt đối cho công trình. Ban đầu nhà thầu đề xuất biện pháp chế tạo 32 cơ cấu vi...

Ngày tải lên: 23/03/2014, 06:20

... it [1,2]. The core histone particle comprises a tetramer of histones H3 and H4 and dimers of histones H 2A and H2B [2]. Each of these histones has a structured core globular domain and an unstructured ... methyltransferases and others may work efficiently in treating diseases caused by epigenetic misregulations. In fact, a combi- natorial therapy targeting histone...

Ngày tải lên: 16/02/2014, 14:20

... agree reasonably with DpK a ¼ 1.2 predicted on the basis of pK a values of phosphate and the cofactor 5¢-phosphate in a model compound. These data are consistent with a pH-depend- ent mechanism ... formation and a ppearance of thermostabilization by phosphate has been examined. The role of the cofactor 5¢-phosphate group in the induction of stability a...

Ngày tải lên: 19/02/2014, 16:20

... recognizes the duplex rather than the component single strands. An additional advantage to modifying the backbone chemistry of the sense strand is that by impairing the incorporation of this strand into ... [13,14]. OAS1 is activated by binding to dsRNA and induces sequence-independent degrada- tion of viral and cellular single-stranded RNA (ssRNA) by activat...

Ngày tải lên: 06/03/2014, 01:23