Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx

... S. Savithri 272 FEBS Journal 278 (2011) 25 7–2 72 ª 2010 The Authors Journal compilation ª 2010 FEBS Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for ... similarity with MPs of other genera, but showed 32% identity with the MP of South-ern bean mosaic virus within the Sobemovirus genus. With a view tounderstanding the mechanism of cell-to-cell movement ... similarity with MPs of viruses from other gen-era. Within sobemoviruses, the sequence of the SeMVMP was closest to that of the SBMV-Ark MP (32%sequence identity), and the identity with MPs of othersobemoviruses...

Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... electrophoresis. The quantity of actin in the pellet was plotted against the totalquantity of actin in the sample.ResultsHeat denaturation of actinBefore starting the investigation of the HSP25–actin ... Accumulation of denatured actin canbe dangerous for the cell as it tends to form high molecularmass aggregates.Let us analyze the interaction of HSP25 with the N-, D1- and D2-forms of actin. ... of muscle cells and of the cytoskeleton system of nonmusclecells. It is therefore a very abundant protein, and itsconcentration in smooth muscle is close to 80 0–9 00 lM[1].Actin has a rather...

Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP.On the other hand, 2-oxoglutarate did not inﬂuence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... analysis of GlnK–TnrA complex formation and ATPeffect on dissociation of the GlnK–TnrA complex. The analyte GlnKwas injected in a volume of 30 lL at a ﬂow rate of 15 lLÆmin)1. The graph shows the ... with ATP and Mg2+or Mn2+. To resolve the inhibitoryeffect of ATP on the GlnK–TnrA interaction in the absence of divalent cations more clearly, ATP wastitrated to the binding assays in the...

Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... one viral protein is responsible for the actual membranefusion step. However, the nature of the interaction of viral fusion proteins with membranes and the mechan-ism by which these proteins ... presence of cholesterol in the lipidbilayer had a minor effect, as there was a shift in kmax of 3 nm for E2( 7–2 6) and 6 nm for W-E2(27 9–2 98).Peptide conformationIn buffer, the CD spectra for the ... showed the characteristics of a random-coil conformation, asindicated by the presence of a negative band atFig. 1. Fluorescence emission spectra of the E2( 7–2 6) (black bro-ken line) and W-E2(27 9–2 98)...

Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... mol ⁄mol). The results are mean ± SD, n ¼ 1–4 . The degree of permea-bilization is expressed as the percentage of the maximal valueobtained at the end of the assay by the addition of 2 mM TritonX-100. ... (TEM) and atomic forcemicroscopy [15].In this study, we measured the interaction of steﬁn B with various combinations of phospholipid monolayers and bilayers. Interaction of steﬁn B in the preﬁbrillaraggregated ... after the end of injection.Discussion The main hypothesis for pathology in AD and otherneurodegenerative diseases is the modiﬁed ‘amyloidTable 1. Critical pressures for the insertion of steﬁns...

Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

... quantify the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a ﬁxed aliquot of the protein was incubated with 1 ng of the ... On the left the western blot with the anti-eEF1A antibody of the protein recovered from the gelband is shown.To completely elucidate the relationship between the structure of the GTs and their ... shown). The cytotoxicity and the formation of the CRC with nuc-lear proteins seem related to the presence of oligomersmigrating according to their length, as demonstratedby electrophoresis for...

Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc

... on the tongue nor the bindingafﬁnity of GA to the receptor has been determined, the need for c-CD in the mm range would correlate with the binding afﬁnity between GA and the receptor. The thermodynamic ... obtained are in the range of those of other interactions with c-CD [15]. The interaction of GA with c-CD is accompanied byfavorable DS values together with favorable DH values(Tables 1 and 2). Because ... should be due to the hydrophobic property of GA, which is also the maindriving force for the interaction with c-CD as describedabove. For the durability of antisweet effect of GA ona human...

Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt

... correlation time of the PSBD–E3 complex. Interaction of PSBD with E3intTo overcome these problems connected with the highmolecular mass of the PSBD–E3 complex, the inter-action of PSBD with a solubilized ... crystallography for the E3–PSBD complex [28].This paper describes the interaction of a15N-labelledPSBD (residues 11 9–1 71) of the B. stearothermophilusE2p polypeptide chain with the dimer of a protein domain ... investigated, as the rigidity or otherwise of the proposed E3-bindingsite is crucial to a proper understanding of protein protein interactions within the multienzyme complex.Further, an improved...

Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf

... C,D, lanes 2 and 3 in both panels). On the other hand, me thyl green did not affect the f ormation of the complexes (lanes 4 and 5 ). The insensitivity of nRLjunRP and rRLjunRP to the major groove ... outin the presence of 1 ng of rad iolab elled Jun-25 and 100 lg(lanes1, 3and5 )and1 50lg (lanes 2, 4 and 6) o f nuclearextracts. Lanes 1 and 2 r e present EM SA performed with nRNE-d, lanes3 and ... 2004 The 5¢ and 3¢ deletion mutants of Jun-25 (Jun-25A a ndJun-25B, respectively) were used i n EMSA to establishwhether the interaction of rRLjunRP at the 3¢ end of the )148 to )124 region of...

Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule ﬂuorescence microscopy pdf

... between the A, B and C chains constituting the six ABCheterotrimeric triple helices of C1q. The cationic sequence 1 4–2 6 of the A chain, as well as the sequence responsible for the kink of the colla-gen-like ... nm. Protein labelingProteins C1q, C1r and the CLR fragments of C1q werelabeled with ﬂuorescent beads. The concentration of beadswas adjusted according to the type and the concentration of protein ... CLR or 2.5 lL of labeled C1, and according to case with 1–2 lLof45 lMfucoidan. Then, the treated DNA wascombed as described below. For the study of the interaction between C1r and C1q bound...

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