Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx

... S. Savithri 272 FEBS Journal 278 (2011) 25 7–2 72 ª 2010 The Authors Journal compilation ª 2010 FEBS Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for ... similarity with MPs of other genera, but showed 32% identity with the MP of South- ern bean mosaic virus within the Sobemovirus genus. With a...

Ngày tải lên: 15/03/2014, 00:20

... electrophoresis. The quantity of actin in the pellet was plotted against the total quantity of actin in the sample. Results Heat denaturation of actin Before starting the investigation of the HSP25–actin ... Accumulation of denatured actin can be dangerous for the cell as it tends to form high molecular mass aggregates. Let us analyze the interaction of HSP25...

Ngày tải lên: 20/02/2014, 23:20

... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP. On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... analysis of GlnK–TnrA complex formation and ATP effect on dissociation of the GlnK–TnrA complex. The analyte GlnK was injected in a volume of 30 lL at a flow rate...

Ngày tải lên: 06/03/2014, 00:21

... one viral protein is responsible for the actual membrane fusion step. However, the nature of the interaction of viral fusion proteins with membranes and the mechan- ism by which these proteins ... presence of cholesterol in the lipid bilayer had a minor effect, as there was a shift in k max of 3 nm for E2( 7–2 6) and 6 nm for W-E2(27 9–2 98). Peptide...

Ngày tải lên: 07/03/2014, 17:20

... mol ⁄ mol). The results are mean ± SD, n ¼ 1–4 . The degree of permea- bilization is expressed as the percentage of the maximal value obtained at the end of the assay by the addition of 2 m M Triton X-100. ... (TEM) and atomic force microscopy [15]. In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayers a...

Ngày tải lên: 07/03/2014, 17:20

... quantify the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a fixed aliquot of the protein was incubated with 1 ng of the ... On the left the western blot with the anti- eEF1A antibody of the protein recovered from the gel band is shown. To completely elucidate the relation...

Ngày tải lên: 16/03/2014, 13:20

... on the tongue nor the binding affinity of GA to the receptor has been determined, the need for c-CD in the mm range would correlate with the binding affinity between GA and the receptor. The thermodynamic ... obtained are in the range of those of other interactions with c-CD [15]. The interaction of GA with c-CD is accompanied by favorable DS values toget...

Ngày tải lên: 16/03/2014, 14:20

... correlation time of the PSBD–E3 complex. Interaction of PSBD with E3int To overcome these problems connected with the high molecular mass of the PSBD–E3 complex, the inter- action of PSBD with a solubilized ... crystallography for the E3–PSBD complex [28]. This paper describes the interaction of a 15 N-labelled PSBD (residues 11 9–1 71) of the B. stearo...

Ngày tải lên: 16/03/2014, 18:20

... C,D, lanes 2 and 3 in both panels). On the other hand, me thyl green did not affect the f ormation of the complexes (lanes 4 and 5 ). The insensitivity of nRLjunRP and rRLjunRP to the major groove ... outin the presence of 1 ng of rad iolab elled Jun-25 and 100 lg(lanes1, 3and5 )and1 50lg (lanes 2, 4 and 6) o f nuclear extracts. Lanes 1 and 2 r e present EM SA...

Ngày tải lên: 16/03/2014, 18:20

... between the A, B and C chains constituting the six ABC heterotrimeric triple helices of C1q. The cationic sequence 1 4–2 6 of the A chain, as well as the sequence responsible for the kink of the colla- gen-like ... nm. Protein labeling Proteins C1q, C1r and the CLR fragments of C1q were labeled with fluorescent beads. The concentration of beads was adjus...

Ngày tải lên: 16/03/2014, 23:20