Báo cáo khoa học: Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast docx
Báo cáo khoa học: Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast docx
... Journal compilation ª 2011 FEBS 1315 Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast Toma´sˇPluskal1, Takeshi ... second and third divisions, the doubling time wasquite uniform for 111 and 4.4 mm glucose, and the stochastic nature of cell division became apparent in 2.2 mm glucose, and prominent in 1.7 and ... CDP-choline and CDP-ethanol-amine (precursors for phosphatidylcholine and phos-phatidylethanolamine, respectively), increased sharply(Fig. 4B).Increase in ergothioneine and trehalose in low glucose Two...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... for PS, PtdIns(3)P, PtdIns(3,4)P2, and PtdIns(3,4,5)P3.Involvement of the CPY-binding sites of IC in itsmembrane bindingAs IC–CPY has no affinity for phospholipid membranes,to obtain ... two binding sites, Met1 and Phe7 in the N-terminal inhibitory reactive site, and His18 in the secondary CPY-binding site arelabeled. (B) The basic (Arg, His, and Lys), acidic (Asp and Glu) and ... additional information on the involvement ofthe CPY-binding sites of IC in its phospholipid recogni-tion, we determined the membrane-binding parameters for the mutant forms of IC, d1–7IC and d1–18IC,...
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Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... bases atthe t erminal. The ÔCCCGGÕ at the 3¢ terminal of the plus-strand 3¢ UTR was important for binding th e NS5Bprotein. Similarly, the binding experiment was p erformedcontaining the RNA competitors ... virus is involved in the binding ofRdRp [34,35]. In addition to NS5B protein, other viralproteins are a ble to b ind the 3¢ UTR, e.g. the H C V NS3protein can bind the 3¢ UTR in vitro and displayed ... single-strandregion is necessary for binding of HCV NS5B protein and initiating RNA s ynthesis fuels the possibility that internalinitiation rather than initiation from the very 3¢ terminal isassociated...
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Báo cáo khoa học: Specific cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot
... thechanges in the thermal unfolding of actin induced byinteraction of G-actin with actin-binding proteins [25–27], G–F transformation of actin, and stabilization ofF-actin by phalloidin and Pianalogs ... effects of actin-bindingproteins on actin structure.Actin-binding proteins profilin and cofilin, whenbound to G-actin between subdomains 1 and 3, haveantagonistic effects on the conformation of ... filament strands and by inducing con-formational changes in actin subunits resulting in thestate of the nucleotide-binding cleft being similar tothat in ATP-actin filaments without phalloidin [32]....
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Báo cáo khoa học: Specific degradation of H. pylori urease by a catalytic antibody light chain pptx
... became very faint. Some newbands between bands 1 and 3 became clear, and sev-eral bands around bands 4 and 5 became stronger.The a-subunit band changed little during this 8 hincubation. Conversely, ... very faint. Some new bands between bands 1 and 2 became clearer and severalbands around bands 4 and 5 also became darker. The band strength of the b-subunit decreased by 65% after 8 h of incubation, ... by HpU-9-L, again indicating thedigestion by HpU-9-L was specific. In summary, we succeeded in the pre-paration of a catalytic antibody light chain capable of specifically digestingthe b-subunit...
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Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf
... structuraldomain constituting the Ca2+binding motif is found in a large number of Ca2+-binding proteins, and isalso known as the EF hand [16]. Proteins containingthe EF hand are divided into two ... classes accordingto their functions: signaling and buffering ⁄ transportproteins. The former undergoing Ca2+-dependentconformational changes, constitute the largest family,including well-known ... To understand the structural origins of the spectral variation, weperformed spectroscopic and structure modeling investigations. For the889 nm form of LH1–RC, bacteriochlorophyll a (BChl a) in...
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Báo cáo khoa học: Specific TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress pptx
... suspensions were obtained in 0.2% methyleneblue, incubated for 1 min, and viable (unstained) cells and dead (stained cells) were counted in Neubauer hemocyto-meter chambers.Bioinformatics and statistical ... signaturemotif and a leucine-zipper domain. The N- and C-ter-mini are least conserved in all proteins. In particular,N-termini are highly heterogeneous, accounting for variability in total protein lengths ... functionally distinctisoforms from a single gene by use of different combi-nations of splice junctions. For example, alternativesplicing within the DNA-binding domain of Pax-6alters DNA-binding specificity...
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Tài liệu Báo cáo khoa học: "An Unsupervised Model for Joint Phrase Alignment and Extraction" ppt
... 5-gram model. For GIZA ++, we use the standard training reg-imen up to Model 4, and combine alignmentswith grow-diag-final -and. For the proposedmodels, we train for 100 iterations, and use the ... removed for TMtraining. For both tasks, we perform weight tuning and testing on specified development and test sets.We compare the accuracy of our proposed methodof joint phrase alignment and extraction ... number of words in each corpus for TM and LM training, tuning, and testing.7.1 Experimental SetupThe data for French, German, and Spanish are fromthe 2010 Workshop on Statistical Machine Transla-tion...
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Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf
... be used to study the involvement of these proteinases in protein degradation and the processing of invariant chain. An assay combi-ning a new monospecific CatE antibody and the substrate, MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-d-Arg-NH2[where ... util-ized a specific inhibitor of CatE, the Ascaris pepsininhibitor, which inhibits pepsins and CatE [33], butdoes not affect other types of aspartic proteinasesincluding CatD [31,34]. This inhibitor ... Katunuma N (2000) Lysosomal cathepsin B plays animportant role in antigen processing, while cathepsin Dis involved in degradation of the invariant chain inoval-bumin-immunized mice. Immunology...
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Báo cáo khoa học: "A Discriminative Model for Joint Morphological Disambiguation and Dependency Parsing" ppt
... resulting in sig-nificantly different training and testing material.Gold Pipeline Parser When given perfect mor-phological information, the Latin parser performs at65.28% accuracy in head selection. ... tense”.Cohen and Smith (2007) separately train a dis-criminative conditional random field (CRF) for seg-mentation and tagging, and a generative probabilis-tic context-free grammar (PCFG) for parsing. ... implemented in the same graphical model framework, and trainedwith the same machine-learning algorithm. Roughlyspeaking, they divide up the variables and factors ofthe joint model and train them...
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