Báo cáo khoa học: Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast docx

... Journal compilation ª 2011 FEBS 1315 Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast Toma ´ s ˇ Pluskal 1 , Takeshi ... second and third divisions, the doubling time was quite uniform for 111 and 4.4 mm glucose, and the stochastic nature of cell division became app...

Ngày tải lên: 14/03/2014, 23:20

... for PS, PtdIns(3)P, PtdIns(3,4)P 2 , and PtdIns(3,4,5)P 3 . Involvement of the CPY-binding sites of I C in its membrane binding As I C –CPY has no affinity for phospholipid membranes, to obtain ... two binding sites, Met1 and Phe7 in the N-terminal inhibitory reactive site, and His18 in the secondary CPY-binding site are labeled. (B) The basic (Arg, His, and Lys), acidic (Asp...

Ngày tải lên: 19/02/2014, 05:20

... bases at the t erminal. The ÔCCCGGÕ at the 3¢ terminal of the plus- strand 3¢ UTR was important for binding th e NS5B protein. Similarly, the binding experiment was p erformed containing the RNA competitors ... virus is involved in the binding of RdRp [34,35]. In addition to NS5B protein, other viral proteins are a ble to b ind the 3¢ UTR, e.g. the H C V NS3 protein can bind the 3¢ UTR...

Ngày tải lên: 19/02/2014, 16:20

... the changes in the thermal unfolding of actin induced by interaction of G-actin with actin-binding proteins [25– 27], G–F transformation of actin, and stabilization of F-actin by phalloidin and P i analogs ... effects of actin-binding proteins on actin structure. Actin-binding proteins profilin and cofilin, when bound to G-actin between subdomains 1 and 3, have antagonistic effects on...

Ngày tải lên: 06/03/2014, 22:21

... became very faint. Some new bands between bands 1 and 3 became clear, and sev- eral bands around bands 4 and 5 became stronger. The a-subunit band changed little during this 8 h incubation. Conversely, ... very faint. Some new bands between bands 1 and 2 became clearer and several bands around bands 4 and 5 also became darker. The band strength of the b-subunit decreased by 65% af...

Ngày tải lên: 07/03/2014, 21:20

... structural domain constituting the Ca 2+ binding motif is found in a large number of Ca 2+ -binding proteins, and is also known as the EF hand [16]. Proteins containing the EF hand are divided into two ... classes according to their functions: signaling and buffering ⁄ transport proteins. The former undergoing Ca 2+ -dependent conformational changes, constitute the largest family, inclu...

Ngày tải lên: 30/03/2014, 02:20

... suspensions were obtained in 0.2% methylene blue, incubated for 1 min, and viable (unstained) cells and dead (stained cells) were counted in Neubauer hemocyto- meter chambers. Bioinformatics and statistical ... signature motif and a leucine-zipper domain. The N- and C-ter- mini are least conserved in all proteins. In particular, N-termini are highly heterogeneous, accounti...

Ngày tải lên: 30/03/2014, 10:20

... 5-gram model. For GIZA ++, we use the standard training reg- imen up to Model 4, and combine alignments with grow-diag-final -and. For the proposed models, we train for 100 iterations, and use the ... removed for TM training. For both tasks, we perform weight tuning and testing on specified development and test sets. We compare the accuracy of our proposed method of joint phr...

Ngày tải lên: 20/02/2014, 04:20

... be used to study the involvement of these proteinases in protein degradation and the processing of invariant chain. An assay combi- ning a new monospecific CatE antibody and the substrate, MOCAc-Gly- Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-d-Arg-NH 2 [where ... util- ized a specific inhibitor of CatE, the Ascaris pepsin inhibitor, which inhibits pepsins and CatE [33], but does not af...

Ngày tải lên: 07/03/2014, 09:20

... resulting in sig- nificantly different training and testing material. Gold Pipeline Parser When given perfect mor- phological information, the Latin parser performs at 65.28% accuracy in head selection. ... tense”. Cohen and Smith (2007) separately train a dis- criminative conditional random field (CRF) for seg- mentation and tagging, and a generative probabilis- tic context-free...

Ngày tải lên: 17/03/2014, 00:20